##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512145_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2039772 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.160108580762948 31.0 30.0 34.0 26.0 34.0 2 30.363017043081285 31.0 30.0 34.0 26.0 34.0 3 30.443426029968055 31.0 30.0 34.0 26.0 34.0 4 34.044564294440754 37.0 35.0 37.0 30.0 37.0 5 33.57274195351245 35.0 35.0 37.0 28.0 37.0 6 33.50632913874688 37.0 35.0 37.0 28.0 37.0 7 33.29693318665027 36.0 35.0 37.0 27.0 37.0 8 33.17921659871789 36.0 35.0 37.0 27.0 37.0 9 34.56996468232724 38.0 35.0 39.0 27.0 39.0 10 34.338038270944004 38.0 34.0 39.0 27.0 39.0 11 34.33094973359768 38.0 34.0 39.0 27.0 39.0 12 34.115063350217575 37.0 34.0 39.0 26.0 39.0 13 34.07744885212661 37.0 34.0 39.0 26.0 39.0 14 34.960558827163034 38.0 34.0 40.0 25.0 41.0 15 34.87479384950867 38.0 34.0 40.0 25.0 41.0 16 34.747801224842775 38.0 34.0 40.0 25.0 41.0 17 34.677745355853496 38.0 34.0 40.0 25.0 41.0 18 34.59045324673542 38.0 34.0 40.0 24.0 41.0 19 34.61138009542243 39.0 34.0 40.0 24.0 41.0 20 34.47236700964618 38.0 34.0 40.0 23.0 41.0 21 34.34508611746803 38.0 34.0 40.0 22.0 41.0 22 34.198166265641454 38.0 34.0 40.0 19.0 41.0 23 34.252533616502234 38.0 34.0 40.0 19.0 41.0 24 34.09687945515479 38.0 34.0 40.0 18.0 41.0 25 33.62778732132807 38.0 33.0 40.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2328541857825357 1101 2 0.18866799889639907 1101 3 0.2778557035234783 1101 4 0.30150595378722755 1101 5 0.39032806534056164 1101 6 0.17561070052617112 1101 7 0.2886227093363374 1101 8 0.18758281544535294 1101 9 0.23261157925822573 1101 10 0.2768533854632125 1101 11 0.35523755433550264 1101 12 0.23946795204602722 1101 13 0.3057873195313121 1101 14 0.24543662329637073 1101 15 0.18133529889507116 1101 16 0.23607311624259353 1101 17 0.2210195518267284 1101 18 0.2772700454913206 1101 19 0.20629312459311677 1101 20 0.17731103445864704 1101 21 0.13578587514190588 1101 22 0.009081736857446288 1101 23 0.13120013494887672 1101 24 -0.20044171878294748 1101 25 -0.1707130400139718 1102 1 0.30024820916668205 1102 2 0.28232916390122753 1102 3 0.34145092863466786 1102 4 0.3496723444535803 1102 5 0.17951902587168433 1102 6 0.201876841541484 1102 7 0.44858223484927606 1102 8 0.38459500782173706 1102 9 0.4444119684937178 1102 10 0.6134895503429618 1102 11 0.6018650139485686 1102 12 0.7352541526974221 1102 13 0.7567626596120576 1102 14 0.835252712030055 1102 15 0.6106773008062305 1102 16 0.7089199385700979 1102 17 0.6297888572565142 1102 18 0.5744733534926638 1102 19 0.5677550590634723 1102 20 0.8202214952874485 1102 21 0.9138255891688942 1102 22 1.0508780236109274 1102 23 1.0194682871809775 1102 24 1.024353782495325 1102 25 0.8875321983552453 1103 1 0.12681909521810297 1103 2 0.12959815694534527 1103 3 0.12256541973152935 1103 4 0.14206543894893997 1103 5 0.1859556382217633 1103 6 0.1389745532696054 1103 7 0.14016542447939173 1103 8 0.2470632800892787 1103 9 0.20415698409116345 1103 10 0.3341934700500033 1103 11 0.10364079818061356 1103 12 0.3093420409383043 1103 13 0.12770586939291917 1103 14 0.23899125613886696 1103 15 0.12854074077057476 1103 16 0.3322831750894437 1103 17 0.18105021626912787 1103 18 0.039450780785294626 1103 19 0.025155613208909244 1103 20 0.20965010546744622 1103 21 0.4993558055956271 1103 22 0.16555222097196065 1103 23 0.0551370399662332 1103 24 0.12435959520966833 1103 25 0.0747597273703775 1104 1 0.42047099341898075 1104 2 0.40197594715720086 1104 3 0.3888647919368182 1104 4 0.42598301615935696 1104 5 0.4403308399993975 1104 6 0.4605065579238641 1104 7 0.4016344413761672 1104 8 0.2923639559792548 1104 9 0.47725312633954076 1104 10 0.41863356256711626 1104 11 0.47861062029439694 1104 12 0.6664441875209377 1104 13 0.6499809364470011 1104 14 0.7802485686549048 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2315 2 -0.8381471439456227 2315 3 -0.8845318457818045 2315 4 -0.8255270472563723 2315 5 -0.938179982169089 2315 6 -1.0977039126823627 2315 7 -1.063200129389969 2315 8 -1.097370680527277 2315 9 -1.324451533638559 2315 10 -1.3078164130387222 2315 11 -1.1628790438296832 2315 12 -1.307459853320367 2315 13 -1.1476200050517775 2315 14 -1.3487473530774423 2315 15 -1.43803682612549 2315 16 -1.6855368556297492 2315 17 -1.5995867153406707 2315 18 -1.5052958869353716 2315 19 -1.4613858940172761 2315 20 -1.5726245859388115 2315 21 -1.360759699327076 2315 22 -1.4676762179099825 2315 23 -1.5381711474999236 2315 24 -1.4468984672012297 2315 25 -1.7068480945354807 2316 1 -1.5759295783721434 2316 2 -1.7925620327784237 2316 3 -1.6448734688571385 2316 4 -1.6695646652591591 2316 5 -1.6599527121577111 2316 6 -1.8159509914908014 2316 7 -1.7999153604964988 2316 8 -1.9581639127058956 2316 9 -2.0827561421607186 2316 10 -2.2222118332856695 2316 11 -2.169996049477831 2316 12 -2.3479596575926145 2316 13 -2.330488414974802 2316 14 -2.5697257962327242 2316 15 -2.418899946189633 2316 16 -2.6805249067745507 2316 17 -2.4601824123919585 2316 18 -2.6148049813123038 2316 19 -2.4530471049012235 2316 20 -2.502124532887123 2316 21 -2.5250666115043217 2316 22 -2.637225997293651 2316 23 -2.615596412136398 2316 24 -2.564263888687819 2316 25 -2.794130128130913 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 48144.0 3 1.0 4 803.0 5 7267.0 6 8983.0 7 7088.0 8 5768.0 9 5253.0 10 4835.0 11 4695.0 12 4455.0 13 4274.0 14 4188.0 15 4104.0 16 4375.0 17 4542.0 18 4849.0 19 5488.0 20 6692.0 21 8333.0 22 11110.0 23 13842.0 24 16457.0 25 19632.0 26 22350.0 27 25568.0 28 29774.0 29 35989.0 30 46012.0 31 57801.0 32 73082.0 33 94337.0 34 120758.0 35 158099.0 36 226634.0 37 374242.0 38 569948.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.660876962049244 20.915102819601245 14.416797691469323 24.007222526880184 2 15.044524584120186 22.373824133285485 39.87107382589819 22.71057745669614 3 18.23365552620587 26.4308952177008 31.28094708624297 24.054502169850355 4 12.982676495216133 18.95947194098164 38.63500430440265 29.422847259399582 5 12.867473756341715 38.482834261138194 36.08976971898034 12.559922263539747 6 30.14219236267583 38.87037374765415 18.509666766677842 12.477767122992176 7 26.525758761273323 32.73900220220691 23.2542656728301 17.480973363689667 8 24.593384429007557 37.144133239005875 21.284079103673427 16.97840322831314 9 26.07232573052282 15.673467426751616 20.872038639612665 37.3821682031129 10 14.461812398640634 30.113071460927987 34.86723025906817 20.557885881363212 11 33.422853142410034 23.29118156342964 25.021767138680207 18.26419815548012 12 22.200226299802132 25.7802832865634 32.331456652998476 19.688033760635992 13 28.111524229178553 22.61836126782797 26.661901428198835 22.608213074794634 14 20.70814777337859 22.573797463638094 28.633347256458073 28.08470750652524 15 22.631352915914132 30.705343538395468 24.49092349537105 22.172380050319347 16 21.790033395889346 29.037951300439463 27.763544160817972 21.408471142853223 17 21.308705090568946 28.779196890632875 28.35611038880816 21.555987629990017 18 21.014015291905174 28.497008489184083 29.800095304769357 20.688880914141382 19 22.708355701062228 27.649944675107818 28.49959970957496 21.142099914255 20 22.645325065742643 27.969939777582987 28.858568506676235 20.52616664999814 21 22.654639832294983 27.349674375371364 28.274385568583156 21.721300223750497 22 22.30847369215775 28.029113057733902 28.620649758894622 21.041763491213725 23 22.17375275275864 27.773496253502845 28.455729365831083 21.597021627907434 24 22.13435886524058 27.660484091197684 28.938787256877013 21.26636978668472 25 22.288178179135137 27.77793302594197 28.72653199296971 21.207356801953186 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 664.0 1 664.0 2 751.0 3 838.0 4 838.0 5 838.0 6 2046.5 7 3255.0 8 3255.0 9 3255.0 10 4644.5 11 6034.0 12 6034.0 13 6034.0 14 9537.0 15 13040.0 16 13040.0 17 13040.0 18 22480.5 19 31921.0 20 31921.0 21 31921.0 22 47959.5 23 63998.0 24 63998.0 25 63998.0 26 89141.0 27 114284.0 28 114284.0 29 114284.0 30 142772.5 31 171261.0 32 171261.0 33 171261.0 34 191247.0 35 211233.0 36 211233.0 37 211233.0 38 224918.5 39 238604.0 40 238604.0 41 238604.0 42 247939.5 43 257275.0 44 257275.0 45 257275.0 46 256835.5 47 256396.0 48 256396.0 49 256396.0 50 244687.5 51 232979.0 52 232979.0 53 232979.0 54 206197.0 55 179415.0 56 179415.0 57 179415.0 58 151014.5 59 122614.0 60 122614.0 61 122614.0 62 97615.0 63 72616.0 64 72616.0 65 72616.0 66 55357.0 67 38098.0 68 38098.0 69 38098.0 70 27205.0 71 16312.0 72 16312.0 73 16312.0 74 11226.0 75 6140.0 76 6140.0 77 6140.0 78 4094.0 79 2048.0 80 2048.0 81 2048.0 82 1307.5 83 567.0 84 567.0 85 567.0 86 353.5 87 140.0 88 140.0 89 140.0 90 86.5 91 33.0 92 33.0 93 33.0 94 19.5 95 6.0 96 6.0 97 6.0 98 3.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40935947743179146 2 0.0 3 0.0 4 0.0 5 0.053388319871044414 6 0.0 7 0.0 8 0.0033827310111130067 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.4707526135273943E-4 20 0.0 21 0.0 22 0.0 23 0.0 24 0.005637885018521678 25 0.0013236773521746548 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2039772.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.88551072996883 #Duplication Level Percentage of deduplicated Percentage of total 1 86.51070227012461 66.51419527646806 2 9.54745595001499 14.681220537775646 3 2.1131207174843745 4.87405096793593 4 0.7521096613033716 2.313053417370144 5 0.34484975218397046 1.3256974660883878 6 0.19155709804955934 0.8836779190494652 7 0.11409680691846733 0.61406738908095 8 0.0805540338163603 0.49547504250640334 9 0.05981975744103594 0.41393453423372106 >10 0.25947474173733415 3.3558506283808462 >50 0.014538645675637714 0.7978145415702749 >100 0.009619926735992993 1.476546025846579 >500 0.0013367699636214886 0.744603887799228 >1k 6.365571255339498E-4 0.9195716082462377 >5k 1.2731142510678995E-4 0.590240757648072 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7037 0.3449895380464091 No Hit TATCAACGCAGAGTACTTTTTTTTT 5023 0.24625301259160337 No Hit GGTATCAACGCAGAGTACTTTTTTT 4649 0.22791763000962856 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2276 0.11158109827961164 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 4.9025087117579805E-5 0.0 0.0 0.0 0.0 9 4.9025087117579805E-5 0.0 0.0 1.4707526135273943E-4 0.0 10 4.9025087117579805E-5 0.0 0.0 1.9610034847031922E-4 0.0 11 4.9025087117579805E-5 0.0 0.0 1.9610034847031922E-4 0.0 12 4.9025087117579805E-5 0.0 0.0 1.9610034847031922E-4 0.0 13 4.9025087117579805E-5 0.0 0.0 1.9610034847031922E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1165 0.0 13.424675 1 TATTCCG 50 0.0014973932 13.302866 5 ATAGGAC 220 0.0 12.957337 3 TAGGACC 405 0.0 12.904016 4 GTCTAGG 115 8.395473E-7 10.7803135 1 TCCAACG 190 9.094947E-12 10.496857 18 AATCCCG 255 0.0 10.428506 19 CCAACGA 120 1.5294863E-6 10.288837 19 TCTACAC 335 0.0 10.211157 3 GTATCAA 2620 0.0 10.191606 1 CCAACGT 75 0.0026542225 10.1305485 19 ACTGTTC 635 0.0 10.020376 8 GATATAC 600 0.0 10.013252 1 AGGACGT 1005 0.0 9.64387 5 GTAGGAC 990 0.0 9.5020485 3 CTAAGAC 200 2.6375346E-10 9.502048 3 CAAGTCG 100 2.7647632E-4 9.496923 6 TATGTCG 90 0.001117382 9.496691 16 AGGACCT 795 0.0 9.4422865 5 TGTAGGA 1110 0.0 9.416444 2 >>END_MODULE