FastQCFastQC Report
Thu 26 May 2016
SRR1512144_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512144_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1245609
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT42150.3383886917965429No Hit
TATCAACGCAGAGTACTTTTTTTTT25280.20295293306326462No Hit
GGTATCAACGCAGAGTACTTTTTTT24210.19436275749452678No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCGA250.00617134718.9246966
GGTTAAC500.001156487713.7655961
GGTATCA8450.013.4979651
GTTCTAT1403.8380676E-1011.93954851
ATAACAC802.9841089E-511.8279343
CCTATTG658.293141E-411.6459663
ACCGAGC752.1510643E-411.3548168
TACCGAT752.1510643E-411.3548167
ATTGGAC951.4206438E-510.9564023
ACCGATC700.001541255910.8141128
CCGATCT803.910409E-410.6451419
TAGGACT1252.3635948E-710.5978294
GTCCTAT951.1605372E-410.3500731
GTATATA2300.010.2600721
TCTATAC1201.6018603E-610.2508763
GTACTAC1354.0162013E-710.1967381
CGATCTT750.002735574710.0931710
TGGACTG2002.7284841E-119.9354655
GTATCAA16950.09.9195821
CATAGAC2300.09.8737543
Produced by FastQC (version 0.11.5)