##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512144_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1245609 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.724484970805445 31.0 30.0 34.0 25.0 34.0 2 27.28472177063589 31.0 27.0 34.0 10.0 34.0 3 28.929474658580663 31.0 28.0 34.0 16.0 34.0 4 33.25131963561599 35.0 33.0 37.0 28.0 37.0 5 33.632933769746366 35.0 35.0 37.0 28.0 37.0 6 33.89599786128713 37.0 35.0 37.0 30.0 37.0 7 33.96374945909993 37.0 35.0 37.0 30.0 37.0 8 34.01461052384818 37.0 35.0 37.0 30.0 37.0 9 35.65397086886816 39.0 37.0 39.0 30.0 39.0 10 35.53577888406394 39.0 37.0 39.0 30.0 39.0 11 35.56487549463756 39.0 37.0 39.0 30.0 39.0 12 35.47506801893692 39.0 37.0 39.0 30.0 39.0 13 35.460267226714 39.0 37.0 39.0 30.0 39.0 14 36.64473522590155 40.0 37.0 41.0 30.0 41.0 15 36.57069995480123 40.0 37.0 41.0 30.0 41.0 16 36.54111442675832 40.0 37.0 41.0 30.0 41.0 17 36.48505911566149 40.0 37.0 41.0 30.0 41.0 18 36.448099684571964 40.0 37.0 41.0 30.0 41.0 19 36.46484249872954 40.0 37.0 41.0 30.0 41.0 20 36.395950896308555 40.0 37.0 41.0 30.0 41.0 21 36.345121141546024 40.0 37.0 41.0 30.0 41.0 22 36.25340536235689 40.0 37.0 41.0 30.0 41.0 23 36.15203807936519 40.0 37.0 41.0 29.0 41.0 24 36.05492092622966 40.0 37.0 41.0 29.0 41.0 25 35.92838121754098 40.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3094267211460746 1101 2 2.8090036181662725 1101 3 1.1302589953395525 1101 4 0.27791053109195474 1101 5 0.12736947898140016 1101 6 -0.33729527339401244 1101 7 -0.4631606393840073 1101 8 -0.4265914358416225 1101 9 -0.5064074807498216 1101 10 -0.8231309571907488 1101 11 -0.634094114198632 1101 12 -0.7056592856247619 1101 13 -0.6993401123628473 1101 14 -0.7272768151443074 1101 15 -0.6740186317453407 1101 16 -0.6052589834620505 1101 17 -0.7686364586923915 1101 18 -0.982571488869695 1101 19 -1.0424342723355622 1101 20 -0.7904428957369518 1101 21 -0.8062923553815438 1101 22 -0.8961671393190827 1101 23 -0.9880846014361495 1101 24 -1.0530092750435571 1101 25 -1.1898188432354573 1102 1 1.2148424343421098 1102 2 3.819148545702504 1102 3 2.3591573281280454 1102 4 1.5659567335546356 1102 5 1.1226402646412055 1102 6 0.9448666531615046 1102 7 0.7424512163422605 1102 8 0.7445647154060566 1102 9 0.7365586301796796 1102 10 0.7245098840427744 1102 11 0.9089809648032201 1102 12 0.7140748322786905 1102 13 0.7904495792575119 1102 14 0.7197211144623168 1102 15 0.7641855744222497 1102 16 0.9115547901022936 1102 17 0.4784377485648861 1102 18 0.595820142382351 1102 19 0.8138913242646382 1102 20 0.8175335671594155 1102 21 0.7936204701605689 1102 22 0.8046283276091089 1102 23 0.686395076018357 1102 24 0.6282591133186557 1102 25 0.7531363708868 1103 1 0.7917896458881124 1103 2 2.816309107315181 1103 3 1.6255715006681868 1103 4 0.9295359498517115 1103 5 0.5665025283481597 1103 6 0.5227147757033279 1103 7 0.2790692143925284 1103 8 0.18674169287208287 1103 9 0.37807337083160775 1103 10 0.2576295684998158 1103 11 0.379122040293737 1103 12 0.276874155474907 1103 13 0.3519591654435388 1103 14 0.42182211958316884 1103 15 0.27202260365703523 1103 16 0.3625907256938774 1103 17 0.4152552020346221 1103 18 0.48193894161590123 1103 19 0.5681745733710102 1103 20 0.66190119263144 1103 21 0.5738318032623297 1103 22 0.5079350295353109 1103 23 0.4722964912554062 1103 24 0.43117362762230016 1103 25 0.3444600601397383 1104 1 1.3342952860381914 1104 2 3.9365063539647593 1104 3 2.420505994790968 1104 4 1.7610220667906376 1104 5 1.2800318766061451 1104 6 0.95371962951576 1104 7 0.9277432365346385 1104 8 0.8039755651592699 1104 9 0.9217226230595585 1104 10 0.9075457036141046 1104 11 0.8202557911521424 1104 12 0.8490319038223717 1104 13 0.7196201531904762 1104 14 0.7728687333227597 1104 15 0.6494641180554197 1104 16 0.844689718536145 1104 17 0.8301231607510644 1104 18 0.7370662748906156 1104 19 0.6794149166661043 1104 20 0.7037217785392471 1104 21 0.7781482571410905 1104 22 1.045015329467752 1104 23 0.9492851241408999 1104 24 0.9810473572668386 1104 25 0.7810805779316752 1105 1 1.0482909204221755 1105 2 3.5708453552940504 1105 3 2.032678970070741 1105 4 1.2257819723272405 1105 5 0.8147760094085257 1105 6 0.6721172119910079 1105 7 0.3794558901890426 1105 8 0.3531198871191279 1105 9 0.2669995974627497 1105 10 0.5487935662211925 1105 11 0.34974503714624205 1105 12 0.11255075180724106 1105 13 0.329567644691366 1105 14 0.357579273373112 1105 15 0.5063924429449074 1105 16 0.27196933999052675 1105 17 0.48652553560003753 1105 18 0.2518046983669038 1105 19 0.3953788268047731 1105 20 0.43561932208727683 1105 21 0.46372817184722237 1105 22 0.5995887015168506 1105 23 0.6402052771969764 1105 24 0.5022684610122141 1105 25 0.5652680514395385 1106 1 0.6235111649922658 1106 2 3.1917491648975513 1106 3 1.6484983276857612 1106 4 0.917924889237959 1106 5 0.5896681284182961 1106 6 0.28246063812390787 1106 7 0.1146537815788804 1106 8 0.04040726295139052 1106 9 0.11964133694659296 1106 10 0.1688486721896112 1106 11 0.20082085865549715 1106 12 0.03563608836007148 1106 13 0.01473087072146484 1106 14 -0.06513072100871398 1106 15 0.0025977814103086416 1106 16 -0.018852109499654546 1106 17 0.06680572733534262 1106 18 0.14416158422224612 1106 19 -0.1050604669781805 1106 20 -0.3149078304522561 1106 21 -0.16920571294424747 1106 22 -0.3250045781847888 1106 23 -0.07326878952786586 1106 24 0.016582863871505538 1106 25 -0.17598791040466466 1107 1 1.1026150979402694 1107 2 3.685128133455155 1107 3 1.9097962829294985 1107 4 1.4156494063011422 1107 5 0.8461543731062804 1107 6 0.8162809302992144 1107 7 0.6662712226317495 1107 8 0.3078712707678477 1107 9 0.32903697432902135 1107 10 0.3923362941745694 1107 11 0.35790746355962 1107 12 0.4320627678972073 1107 13 0.3235808866133496 1107 14 0.5506911250400961 1107 15 0.6359371968242655 1107 16 0.542647731581468 1107 17 0.5457295522185035 1107 18 0.37284349925361226 1107 19 0.632476761241108 1107 20 0.5485779080496656 1107 21 0.6165828189984452 1107 22 0.5196116032888014 1107 23 0.8100835748319284 1107 24 0.7228301416499434 1107 25 0.8605922729994973 1108 1 0.7169559859376342 1108 2 2.379819909150495 1108 3 1.4232210306915398 1108 4 0.8709830789456703 1108 5 0.7625490918272817 1108 6 0.766629722251551 1108 7 0.6726033683758885 1108 8 0.6469209998178798 1108 9 0.7211539768139801 1108 10 0.6510980078957118 1108 11 0.6974481958730223 1108 12 0.6704658097223017 1108 13 0.7827886947628073 1108 14 0.6936598984301128 1108 15 0.7180544987014557 1108 16 0.7363964217404444 1108 17 0.8634415967680695 1108 18 0.6061688487940486 1108 19 0.6215758900097086 1108 20 0.5941424121064287 1108 21 0.7296692535363363 1108 22 0.9383385875108274 1108 23 0.9018293309470167 1108 24 1.0065611676671224 1108 25 0.9060639654637939 1109 1 0.676400129053949 1109 2 3.064550054224405 1109 3 1.4730156080320818 1109 4 0.9869212588822052 1109 5 0.5815059561859783 1109 6 0.37732666304368934 1109 7 0.23008181302399322 1109 8 0.17687530184841194 1109 9 0.11646385642819723 1109 10 -0.06071862666392036 1109 11 0.10637706788753576 1109 12 -0.11441146660390444 1109 13 0.03566118308894062 1109 14 0.21703506442035092 1109 15 0.22534560037787799 1109 16 0.008384750990259704 1109 17 0.028912510291050353 1109 18 -0.03676666906432047 1109 19 -0.0425103688041375 1109 20 -0.08240511565212216 1109 21 -0.011336101791876274 1109 22 -0.1447724903502774 1109 23 0.0886423807409713 1109 24 -0.030638725101624686 1109 25 -0.06352225906098852 1110 1 -0.1469407869332109 1110 2 1.314189443308603 1110 3 0.4973245525356873 1110 4 -0.1904342438889728 1110 5 -0.026937911882285448 1110 6 -0.015920587519531182 1110 7 -0.07842461032775105 1110 8 -0.2966308283760952 1110 9 -0.16791594111430186 1110 10 -0.22789778280539252 1110 11 -0.3304997548967563 1110 12 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-1.7913359517706482 2315 21 -1.8135124062783134 2315 22 -1.8655426751189523 2315 23 -1.771712582508492 2315 24 -1.6975709485617116 2315 25 -1.7786688057716589 2316 1 -1.3477515506202984 2316 2 -1.7811334091847115 2316 3 -1.0343577094659402 2316 4 -1.4207913867461173 2316 5 -1.3676322917497714 2316 6 -1.5589397378159404 2316 7 -1.4240431445599953 2316 8 -1.7701909236916862 2316 9 -1.8397571592940096 2316 10 -1.9498792137015855 2316 11 -2.3001825690313638 2316 12 -2.013462761706272 2316 13 -2.27924027036655 2316 14 -2.501566479522644 2316 15 -2.6885399894875235 2316 16 -2.6282478414766572 2316 17 -2.3850960860215835 2316 18 -2.1291798576176504 2316 19 -2.4225748411489008 2316 20 -2.7864607937428403 2316 21 -2.79322163510259 2316 22 -2.671405506977358 2316 23 -2.7152966533049536 2316 24 -2.8100867513430003 2316 25 -2.9662715238500397 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38731.0 3 0.0 4 329.0 5 1526.0 6 1793.0 7 1669.0 8 1536.0 9 1451.0 10 1348.0 11 1356.0 12 1359.0 13 1370.0 14 1372.0 15 1385.0 16 1530.0 17 1572.0 18 1743.0 19 2356.0 20 2856.0 21 3634.0 22 4452.0 23 5302.0 24 6208.0 25 7126.0 26 8091.0 27 9503.0 28 11279.0 29 14098.0 30 18070.0 31 23618.0 32 32528.0 33 45293.0 34 64250.0 35 95697.0 36 146646.0 37 217378.0 38 467154.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.22777773763677 18.69101780735367 13.539642054609432 25.541562400400124 2 16.151905069758712 21.454818460511774 38.65704573418699 23.73623073554253 3 17.869411669311962 24.843831410980492 30.25829132576916 27.02846559393839 4 12.646424359490016 17.290257215546774 37.90667858051764 32.156639844445564 5 13.586366187142193 37.22275609761972 35.32914421780832 13.861733497429771 6 30.95192793244108 36.82030235812362 18.533825622647235 13.693944086788068 7 27.105456045998384 31.968458802079947 22.909917959809217 18.016167192112455 8 25.137764047758203 34.98693007752037 21.32589064938568 18.54941522533574 9 25.881315886445904 15.62328146312366 20.63601017654818 37.85939247388225 10 15.290592794368054 28.24088457935034 33.842963562402005 22.625559063879596 11 32.92277111035646 23.058359404917596 24.74958032576836 19.269289158957587 12 22.641374620767834 24.684632175907527 31.41547628509428 21.25851691823036 13 27.651614591737854 21.915785772260797 26.561866524728067 23.87073311127328 14 21.483805499001292 21.44101515083397 27.80200512520793 29.27317422495681 15 22.98433938740006 29.04418641804932 24.334843438029107 23.63663075652151 16 22.586060312666334 27.920800186896532 26.70428681873686 22.788852681700277 17 21.733625880994758 27.751244571932283 27.714555691232164 22.800573855840796 18 21.651336815967127 27.34140488708736 28.73204994504696 22.27520835189855 19 22.822421893314406 27.001518941814574 28.11045886172491 22.065600303146105 20 22.90136455559418 26.817848647286024 28.31647542122034 21.964311375899463 21 22.971574547068943 26.382677068004483 27.946650995617407 22.699097389309163 22 22.88180565044505 26.539397447497233 27.96545603287088 22.613340869186842 23 22.570827369000778 26.633772185109333 27.923834235576617 22.871566210313272 24 22.575999152219882 26.498025860504846 28.093233333815025 22.832741653460243 25 22.573536318379205 26.460309776181774 28.15040674882728 22.815747156611746 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 620.0 1 620.0 2 598.5 3 577.0 4 577.0 5 577.0 6 1233.0 7 1889.0 8 1889.0 9 1889.0 10 2592.0 11 3295.0 12 3295.0 13 3295.0 14 4901.0 15 6507.0 16 6507.0 17 6507.0 18 11313.0 19 16119.0 20 16119.0 21 16119.0 22 24050.5 23 31982.0 24 31982.0 25 31982.0 26 44387.0 27 56792.0 28 56792.0 29 56792.0 30 71276.5 31 85761.0 32 85761.0 33 85761.0 34 97969.5 35 110178.0 36 110178.0 37 110178.0 38 121703.5 39 133229.0 40 133229.0 41 133229.0 42 142390.0 43 151551.0 44 151551.0 45 151551.0 46 155925.0 47 160299.0 48 160299.0 49 160299.0 50 158024.0 51 155749.0 52 155749.0 53 155749.0 54 141564.5 55 127380.0 56 127380.0 57 127380.0 58 108745.0 59 90110.0 60 90110.0 61 90110.0 62 73930.5 63 57751.0 64 57751.0 65 57751.0 66 45066.0 67 32381.0 68 32381.0 69 32381.0 70 23673.5 71 14966.0 72 14966.0 73 14966.0 74 10343.5 75 5721.0 76 5721.0 77 5721.0 78 3833.5 79 1946.0 80 1946.0 81 1946.0 82 1261.5 83 577.0 84 577.0 85 577.0 86 372.0 87 167.0 88 167.0 89 167.0 90 106.0 91 45.0 92 45.0 93 45.0 94 29.0 95 13.0 96 13.0 97 13.0 98 8.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.7599278746380285 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 8.028201466110152E-5 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 8.028201466110152E-5 15 0.0 16 0.0 17 0.0 18 0.0 19 4.014100733055076E-4 20 1.6056402932220303E-4 21 0.0 22 6.422561172888121E-4 23 8.028201466110152E-4 24 2.4084604398330457E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1245609.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.8086167318441 #Duplication Level Percentage of deduplicated Percentage of total 1 85.86828460028919 65.09555876684232 2 9.522565388074765 14.43785019616968 3 2.299549100046387 5.229769091444206 4 0.8769028575295987 2.6590717055008097 5 0.44170542382780986 1.67425385916696 6 0.2589427835359056 1.1778056539530202 7 0.15668568045544318 0.831468728791042 8 0.11484219367035645 0.696482227568021 9 0.07640194834826616 0.5212733417909873 >10 0.3613353039376008 4.606077568238366 >50 0.014031619768873155 0.7324084449503243 >100 0.007578917305723789 1.1175284546499822 >500 6.404635691504121E-4 0.30313042339607366 >1k 5.337196409586767E-4 0.9173215375381448 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4215 0.3383886917965429 No Hit TATCAACGCAGAGTACTTTTTTTTT 2528 0.20295293306326462 No Hit GGTATCAACGCAGAGTACTTTTTTT 2421 0.19436275749452678 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 8.028201466110152E-5 0.0 0.0 0.0 0.0 6 8.028201466110152E-5 0.0 0.0 0.0 0.0 7 1.6056402932220303E-4 0.0 0.0 0.0 0.0 8 1.6056402932220303E-4 0.0 0.0 0.0 0.0 9 1.6056402932220303E-4 0.0 0.0 0.0 0.0 10 1.6056402932220303E-4 0.0 0.0 0.0 0.0 11 1.6056402932220303E-4 0.0 0.0 0.0 0.0 12 3.2112805864440607E-4 0.0 0.0 0.0 0.0 13 3.2112805864440607E-4 0.0 0.0 0.0 8.028201466110152E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAACCGA 25 0.006171347 18.924696 6 GGTTAAC 50 0.0011564877 13.765596 1 GGTATCA 845 0.0 13.497965 1 GTTCTAT 140 3.8380676E-10 11.9395485 1 ATAACAC 80 2.9841089E-5 11.827934 3 CCTATTG 65 8.293141E-4 11.645966 3 ACCGAGC 75 2.1510643E-4 11.354816 8 TACCGAT 75 2.1510643E-4 11.354816 7 ATTGGAC 95 1.4206438E-5 10.956402 3 ACCGATC 70 0.0015412559 10.814112 8 CCGATCT 80 3.910409E-4 10.645141 9 TAGGACT 125 2.3635948E-7 10.597829 4 GTCCTAT 95 1.1605372E-4 10.350073 1 GTATATA 230 0.0 10.260072 1 TCTATAC 120 1.6018603E-6 10.250876 3 GTACTAC 135 4.0162013E-7 10.196738 1 CGATCTT 75 0.0027355747 10.09317 10 TGGACTG 200 2.7284841E-11 9.935465 5 GTATCAA 1695 0.0 9.919582 1 CATAGAC 230 0.0 9.873754 3 >>END_MODULE