FastQCFastQC Report
Thu 26 May 2016
SRR1512144_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512144_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1245609
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59640.47880193543880945No Hit
TATCAACGCAGAGTACTTTTTTTTT43970.3530000184648634No Hit
GGTATCAACGCAGAGTACTTTTTTT41240.33108302846238263No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19930.16000205521957533No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15820.1270061471938626No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13780.1106286162029979No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCGT602.5735477E-514.2464176
GGTATCA12200.013.9023771
GTATTAT1251.7502316E-811.4343151
CTTAGGC752.0664377E-411.4043
GGACCGT600.00588555411.0805476
TAGGACC1658.0035534E-1110.94323354
CTAATAC700.001486683310.8609533
TCATACT1303.8187864E-710.2343614
TAAGACT1409.6266376E-810.1821444
CTATACC750.00263924510.1368894
TTTAGAC856.5697567E-410.0623533
AACCGTG856.6062424E-410.0562947
ACCGTGT1151.028684E-59.9105518
TCTATAC1252.5559784E-69.8834683
GTAGGAC4450.09.8236713
TAGACTA800.0045013359.5033345
TTCATAC800.0045013359.5033343
GTAAGAC1201.6919324E-59.5033343
TAGTACC1002.7441603E-49.5033344
GTATCAA23600.09.488221
Produced by FastQC (version 0.11.5)