FastQCFastQC Report
Thu 26 May 2016
SRR1512143_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512143_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81570
Sequences flagged as poor quality0
Sequence length25
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA995512.204241755547383No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT78579.632217727105553No Hit
GTATCAACGCAGAGTACTTTTTTTT31343.8420988108373173No Hit
TATCAACGCAGAGTACTTTTTTTTT21782.6700993012136816No Hit
GGTATCAACGCAGAGTACTTTTTTT21152.5928650239058477No Hit
GGTATCAACGCAGAAAAAAAAAAAA20072.460463405663847No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14091.7273507416942502No Hit
GGTATCAACGCAGAGAAAAAAAAAA12531.5361039597891382No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11181.3706019369866371No Hit
GTATCAACGCAGAGAAAAAAAAAAA11091.359568468799804No Hit
GAGTACTTTTTTTTTTTTTTTTTTT9361.147480691430673No Hit
GTATCAACGCAGAAAAAAAAAAAAA9041.108250582321932No Hit
ANAAAAAAAAAAAAAAAAAAAAAAA7580.9292632095133014No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA7050.8642883413019492No Hit
TATCAACGCAGAGAAAAAAAAAAAA6960.8532548731151158No Hit
TNTTTTTTTTTTTTTTTTTTTTTTT6110.7490498957950227No Hit
GTACATAAGCAGTGGTATCAACGCA4890.5994851048179478No Hit
GCAGAGTACTTTTTTTTTTTTTTTT4560.5590290547995587No Hit
TATCAACGCAGAAAAAAAAAAAAAA4470.5479955866127252No Hit
ATCAACGCAGAGTACTTTTTTTTTT3740.45850190020841No Hit
GTGGTATCAACGCAGAGTACTTTTT3360.4119161456417801No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT3200.3923010910874096No Hit
AGAAAAAAAAAAAAAAAAAAAAAAA3140.38494544562952066No Hit
GAGAAAAAAAAAAAAAAAAAAAAAA2870.3518450410690205No Hit
GTGGTATCAACGCAGAAAAAAAAAA2840.348167218340076No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA2680.32855216378570556No Hit
ACGCAGAGAAAAAAAAAAAAAAAAA2460.3015814637734461No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT2380.2917739364962609No Hit
TATTTTTTTTTTTTTTTTTTTTTTT2070.2537697682971681No Hit
ACGCAGAAAAAAAAAAAAAAAAAAA2010.24641412283927916No Hit
GCTTATGTACTCTGCGTTGATACCA2000.245188181929631No Hit
GNATCAACGCAGAGTACTTTTTTTT1990.24396224101998285No Hit
TCTTTTTTTTTTTTTTTTTTTTTTT1850.22679906828490867No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT1820.2231212455559642No Hit
GTGGTATCAACGCAGAGAAAAAAAA1800.2206693637366679No Hit
ATAAAAAAAAAAAAAAAAAAAAAAA1790.21944342282701973No Hit
GTGGTATCAACGCAGAGTACATGGG1750.2145396591884271No Hit
GNTATCAACGCAGAAAAAAAAAAAA1560.19124678190511218No Hit
TNTCAACGCAGAGTACTTTTTTTTT1500.18389113644722324No Hit
AACGCAGAGTACTTTTTTTTTTTTT1490.18266519553757507No Hit
GNTATCAACGCAGAGTACTTTTTTT1400.1716317273507417No Hit
CTTATGTACTCTGCGTTGATACCAC1400.1716317273507417No Hit
GAATCAACGCAGAGTACTTTTTTTT1290.15814637734461198No Hit
GTACATGGGAAAAAAAAAAAAAAAA1240.1520166727963712No Hit
CAACGCAGAGTACTTTTTTTTTTTT1220.1495647909770749No Hit
TCAACGCAGAGTACTTTTTTTTTTT1210.14833885006742675No Hit
GTATCAACGCAGAGTAAAAAAAAAA1170.14343508642883412No Hit
GGTATCAACGCAGAGTACATGGGGG1130.1385313227902415No Hit
GNTATCAACGCAGAGAAAAAAAAAA1130.1385313227902415No Hit
GNACTTTTTTTTTTTTTTTTTTTTT1110.13607944097094518No Hit
GAGTAAAAAAAAAAAAAAAAAAAAA1100.13485350006129704No Hit
GTACATGGGGAAAAAAAAAAAAAAA1090.1336275591516489No Hit
GCATCAACGCAGAGTACTTTTTTTT1090.1336275591516489No Hit
CCCATGTACTCTGCGTTGATACCAC1060.12994973642270444No Hit
TATGTACTCTGCGTTGATACCACTG1020.1250459727841118No Hit
GAGTACATAAGCAGTGGTATCAACG1010.12382003187446364No Hit
GGATCAACGCAGAGTACTTTTTTTT1000.1225940909648155No Hit
GNATCAACGCAGAGAAAAAAAAAAA960.11769032732622288No Hit
GGTATCAACGCAGAGTAAAAAAAAA950.11646438641657471No Hit
GTATCAACGCAGAGTACATGGGGAA950.11646438641657471No Hit
TATCAACGCAGAGTAAAAAAAAAAA940.11523844550692657No Hit
ATCAACGCAGAGAAAAAAAAAAAAA930.11401250459727841No Hit
GCGTTGATACCACTGCTTATGTACT930.11401250459727841No Hit
GGTATCAACGCAGAGTACATGGGGA900.11033468186833395No Hit
ANGCAGAGTACTTTTTTTTTTTTTT880.10788280004903764No Hit
GAGTACATGGGAAAAAAAAAAAAAA860.10543091822974134No Hit
GTATCAACGCAGAGTACATGGGAAA850.10420497732009316No Hit
GNATCAACGCAGAAAAAAAAAAAAA840.10297903641044502No Hit
GGTATCAACGCAGAGTACATGGGAA840.10297903641044502No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCAAC250.004365680320.0631921
GGTATCA9650.012.1626621
ATGGGGA1701.7378079E-78.8857795
TGGGGAA1000.00300883628.4970266
TACATAA1209.6051703E-48.3596642
GTATCAA16300.08.2468341
CGCAGAA5350.07.9411479
GCAGAAA5350.07.94114710
CAGAAAA5400.07.86761711
GGGGAAA1100.0070976067.7245697
CATAAGC1356.505469E-47.69278144
ACATAAG1250.00261777487.5529123
GTACATA1350.00306587397.4308121
TGGTATC3302.5520421E-96.9917192
GTGGTAT3404.7566573E-96.786081
AGAGAAA3754.2018655E-106.545857412
ATCAACG22550.06.5313433
TATCAAC20650.06.50960732
ATAAGCA1600.00392854266.49078375
CATGGGG3351.240187E-86.48197754
Produced by FastQC (version 0.11.5)