##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512143_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 81570 Sequences flagged as poor quality 0 Sequence length 25 %GC 27 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.45222508275101 31.0 28.0 33.0 16.0 34.0 2 25.22492337869315 31.0 18.0 33.0 2.0 34.0 3 26.911511585141596 31.0 25.0 33.0 16.0 34.0 4 31.575763148216257 35.0 32.0 37.0 19.0 37.0 5 32.16379796493809 35.0 33.0 37.0 19.0 37.0 6 32.634473458379304 35.0 33.0 37.0 25.0 37.0 7 32.786772097584894 37.0 35.0 37.0 25.0 37.0 8 32.8515753340689 37.0 35.0 37.0 25.0 37.0 9 34.1696702218953 39.0 35.0 39.0 25.0 39.0 10 34.173470638715216 39.0 35.0 39.0 25.0 39.0 11 34.107637611867105 39.0 35.0 39.0 25.0 39.0 12 34.02797597155817 39.0 35.0 39.0 23.0 39.0 13 33.862535245801155 39.0 35.0 39.0 23.0 39.0 14 34.787434105676105 39.0 34.0 41.0 22.0 41.0 15 34.62431040823832 39.0 34.0 41.0 19.0 41.0 16 34.69710677945323 39.0 34.0 41.0 20.0 41.0 17 34.52565894323894 38.0 35.0 40.0 23.0 41.0 18 34.159568468799804 37.0 35.0 40.0 23.0 41.0 19 33.94885374524948 37.0 34.0 40.0 23.0 41.0 20 33.854505332842955 36.0 34.0 40.0 23.0 41.0 21 33.77651097217114 36.0 34.0 40.0 22.0 41.0 22 33.60386171386539 36.0 34.0 40.0 21.0 41.0 23 33.35723918107147 35.0 33.0 40.0 18.0 41.0 24 33.22898124310408 35.0 33.0 40.0 18.0 41.0 25 33.08841485840382 35.0 33.0 40.0 17.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -1.1290359478120742 1101 2 2.0240261497524052 1101 3 -0.0522476053495744 1101 4 -0.2832918806293243 1101 5 -0.7483026414067702 1101 6 -1.4181905588055237 1101 7 -0.9945005685123043 1101 8 -0.7510759323579421 1101 9 -2.1175966712709666 1101 10 -1.465805238764311 1101 11 -1.068632255570499 1101 12 -1.7272547213617813 1101 13 -1.4986504574942785 1101 14 -1.5860914753289066 1101 15 -1.5487637382961026 1101 16 -2.0402531187235198 1101 17 -1.6890163022352525 1101 18 -1.9782763434937323 1101 19 -2.090222491439821 1101 20 -2.015155463915004 1101 21 -2.2238189715380123 1101 22 -2.2896282552268588 1101 23 -1.516080923112714 1101 24 -1.3038012343769765 1101 25 -1.6706521691816043 1102 1 1.3812477401311902 1102 2 4.701331114291413 1102 3 3.2445609052887256 1102 4 2.236211665469966 1102 5 1.9885767912173407 1102 6 1.5520222071519214 1102 7 1.8058540414167723 1102 8 2.1989240676420607 1102 9 1.8324033287290362 1102 10 1.9997975981151228 1102 11 2.6593819288266616 1102 12 2.2539509523970835 1102 13 2.6073779113000484 1102 14 2.9085893757349197 1102 15 2.597335552484033 1102 16 2.6547823422693924 1102 17 2.1280049743604934 1102 18 2.453638550123287 1102 19 2.801621480191386 1102 20 2.8110856708367677 1102 21 2.6137696809442588 1102 22 2.4546979859078917 1102 23 1.7856921265326733 1102 24 1.7323689783889833 1102 25 2.423319462024068 1103 1 0.16004451195804137 1103 2 2.0700031612466603 1103 3 1.7460282567193914 1103 4 0.627627659600563 1103 5 -0.44025666439527456 1103 6 -0.5837078001848326 1103 7 -0.5008224075927643 1103 8 -0.9045242082200069 1103 9 -0.6848380505813054 1103 10 -1.3043109858907442 1103 11 -0.7232299567199263 1103 12 -1.1473696638905224 1103 13 -1.0371562046207146 1103 14 -1.5774707856737322 1103 15 -1.4602579911696694 1103 16 -0.8793335784936289 1103 17 -0.9545335436145592 1103 18 -1.1817246193558049 1103 19 -0.7310270891409729 1103 20 -0.589868107593162 1103 21 -0.9284166726874403 1103 22 -0.7419271058015724 1103 23 -1.2304487392046681 1103 24 -1.7900081309286975 1103 25 -1.5183533186068914 1104 1 0.2876307188545937 1104 2 2.940692816419073 1104 3 1.9810857279837606 1104 4 0.5833747860373393 1104 5 -0.0649693080734366 1104 6 -0.23485722547219012 1104 7 -0.16116723517897213 1104 8 0.11559073430872502 1104 9 -9.300046042994836E-4 1104 10 -0.4491385720976382 1104 11 -0.8852989222371619 1104 12 -1.2272547213617813 1104 13 -0.4319837908276085 1104 14 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24 -0.012020412459165186 1108 25 0.5839140408640588 1109 1 -0.03736928114540561 1109 2 2.2031928164190724 1109 3 0.9477523946504256 1109 4 0.6250414527040107 1109 5 0.18086402525989342 1109 6 -0.5056905588055258 1109 7 -0.3111672351789707 1109 8 -0.45107593235793786 1109 9 -0.7675966712709652 1109 10 -0.524138572097641 1109 11 -0.5727989222371619 1109 12 -0.2439213880284541 1109 13 -0.6819837908276085 1109 14 -0.748591475328908 1109 15 -1.465430404962774 1109 16 -1.3694197853901855 1109 17 -0.37651630223525245 1109 18 -0.31577634349373085 1109 19 0.030610841893512486 1109 20 -0.49848879724833495 1109 21 -0.12798563820467734 1109 22 0.20203841143980839 1109 23 -0.4869142564460489 1109 24 0.05869876562302778 1109 25 -0.099818835848275 1110 1 0.3047038895862997 1110 2 2.4589854993459035 1110 3 0.858728004406526 1110 4 0.5195536478259584 1110 5 0.9281201228208715 1110 6 0.23546797777984096 1110 7 0.5546864233576159 1110 8 1.2842899213005978 1110 9 0.5275252799485486 1110 10 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0.1092210246710934 2315 15 0.27519459503722743 2315 16 -0.3553572853901841 2315 17 0.4562961977647504 2315 18 0.4373486565062663 2315 19 -0.29595165810648894 2315 20 -0.18442629724833637 2315 21 -0.36079813820467876 2315 22 -0.15733658856019161 2315 23 -0.08847675644604891 2315 24 -0.25848873437697506 2315 25 -0.5560688358482722 2316 1 -0.5461530649291895 2316 2 -0.802550426824169 2316 3 0.09504969194772528 2316 4 -0.5377963851338308 2316 5 -0.600554893659023 2316 6 -0.6249473155622809 2316 7 -0.14900507301680932 2316 8 -0.6186434999255042 2316 9 -0.2621912658655603 2316 10 -0.5207601937192621 2316 11 -0.8690827060209472 2316 12 -1.746624090731153 2316 13 -1.3279297367735552 2316 14 -1.4712266104640435 2316 15 -1.8654304049627726 2316 16 -1.4298927583631595 2316 17 -1.5768541400730882 2316 18 -1.875573640791032 2316 19 -1.567699968917303 2316 20 -1.5038942026537399 2316 21 -2.2769721246911665 2316 22 -2.2540426696412723 2316 23 -1.9886034456352384 2316 24 -2.528125558701298 2316 25 -2.5805620790915142 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4264.0 3 0.0 4 33.0 5 147.0 6 154.0 7 166.0 8 125.0 9 133.0 10 130.0 11 125.0 12 123.0 13 108.0 14 125.0 15 133.0 16 173.0 17 129.0 18 194.0 19 228.0 20 328.0 21 379.0 22 461.0 23 557.0 24 646.0 25 713.0 26 812.0 27 889.0 28 1191.0 29 1372.0 30 1724.0 31 2208.0 32 2844.0 33 3991.0 34 5802.0 35 8219.0 36 16183.0 37 13157.0 38 13604.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.09832046095378 25.3487801887949 22.00073556454579 8.55216378570553 2 16.38748502058042 31.976501849632676 38.31084249465951 13.325170635127389 3 15.045972784111806 39.29753585877161 36.63969596665441 9.01679539046218 4 7.8975113399534145 32.66887336030403 36.17138653916881 23.26222876057374 5 5.326713252421234 43.76731641534878 36.52690940296678 14.37906092926321 6 14.739487556699768 44.7198725021454 23.289199460585998 17.251440480568835 7 11.74696579624862 50.058845163663115 25.73985533897266 12.454333701115607 8 18.590167953904622 39.07686649503494 24.572759592987616 17.760205958072824 9 21.477258796126026 25.888194189040085 24.802010543091825 27.832536471742063 10 18.010297903641046 36.84442809856565 26.99154100772343 18.15373299006988 11 18.43202157656001 36.87998038494544 24.667157043030528 20.020840995464017 12 21.098443055044747 42.67500306485228 28.243226676474194 7.983327203628784 13 25.045972784111807 36.01201422091455 29.410322422459238 9.531690572514405 14 17.709942380777246 41.418413632462915 31.731028564423198 9.140615422336644 15 18.519063381145028 40.19124678190511 33.03052592865024 8.25916390829962 16 9.219075640554125 46.34301826651955 32.72894446487679 11.708961628049527 17 9.106289076866494 45.03984307956356 32.3930366556332 13.460831187936742 18 8.037268603653304 40.94152261860978 40.139757263699885 10.881451514037023 19 9.128356013240161 42.415103592006865 40.64852274120388 7.808017653549099 20 9.992644354542112 40.61542233664338 41.385313227902415 8.0066200809121 21 10.44501655020228 41.49809979159004 40.574966286624985 7.48191737158269 22 9.76094152261861 41.17567733235258 40.74292019124678 8.320460953782028 23 9.947284540885128 41.04817947774917 40.94029667770013 8.064239303665563 24 8.71031016305014 42.010543091822974 40.432757141105796 8.846389604021086 25 8.897879122226309 42.78533774672061 40.7024641412284 7.614318989824691 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 19739.0 1 19739.0 2 11518.0 3 3297.0 4 3297.0 5 3297.0 6 2896.5 7 2496.0 8 2496.0 9 2496.0 10 1965.0 11 1434.0 12 1434.0 13 1434.0 14 1059.0 15 684.0 16 684.0 17 684.0 18 1411.0 19 2138.0 20 2138.0 21 2138.0 22 3480.5 23 4823.0 24 4823.0 25 4823.0 26 6617.0 27 8411.0 28 8411.0 29 8411.0 30 8091.5 31 7772.0 32 7772.0 33 7772.0 34 6412.0 35 5052.0 36 5052.0 37 5052.0 38 4121.5 39 3191.0 40 3191.0 41 3191.0 42 4088.0 43 4985.0 44 4985.0 45 4985.0 46 4896.0 47 4807.0 48 4807.0 49 4807.0 50 4642.5 51 4478.0 52 4478.0 53 4478.0 54 3835.5 55 3193.0 56 3193.0 57 3193.0 58 2650.0 59 2107.0 60 2107.0 61 2107.0 62 1798.5 63 1490.0 64 1490.0 65 1490.0 66 1137.0 67 784.0 68 784.0 69 784.0 70 601.0 71 418.0 72 418.0 73 418.0 74 306.0 75 194.0 76 194.0 77 194.0 78 123.5 79 53.0 80 53.0 81 53.0 82 33.0 83 13.0 84 13.0 85 13.0 86 10.0 87 7.0 88 7.0 89 7.0 90 5.0 91 3.0 92 3.0 93 3.0 94 1.5 95 0.0 96 0.0 97 0.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.882064484491847 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 81570.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.572269216623756 #Duplication Level Percentage of deduplicated Percentage of total 1 85.26874279123415 22.6578398921172 2 7.4878892733564015 3.9794041927179107 3 2.362168396770473 1.883045237219566 4 1.2087658592848904 1.2847860733112664 5 0.5813148788927336 0.7723427730783377 6 0.43367935409457903 0.6914306730415594 7 0.35063437139561704 0.6522005639328184 8 0.20761245674740486 0.4413387274733358 9 0.13379469434832758 0.3199705774181685 >10 1.4625144175317186 7.819051121735933 >50 0.2445213379469435 4.534755424788525 >100 0.1845444059976932 9.916636018143926 >500 0.02768166089965398 5.651587593477994 >1k 0.03690888119953864 17.559151648890523 >5k 0.00922722029988466 21.836459482652938 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAA 9955 12.204241755547383 No Hit TTTTTTTTTTTTTTTTTTTTTTTTT 7857 9.632217727105553 No Hit GTATCAACGCAGAGTACTTTTTTTT 3134 3.8420988108373173 No Hit TATCAACGCAGAGTACTTTTTTTTT 2178 2.6700993012136816 No Hit GGTATCAACGCAGAGTACTTTTTTT 2115 2.5928650239058477 No Hit GGTATCAACGCAGAAAAAAAAAAAA 2007 2.460463405663847 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1409 1.7273507416942502 No Hit GGTATCAACGCAGAGAAAAAAAAAA 1253 1.5361039597891382 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1118 1.3706019369866371 No Hit GTATCAACGCAGAGAAAAAAAAAAA 1109 1.359568468799804 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 936 1.147480691430673 No Hit GTATCAACGCAGAAAAAAAAAAAAA 904 1.108250582321932 No Hit ANAAAAAAAAAAAAAAAAAAAAAAA 758 0.9292632095133014 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 705 0.8642883413019492 No Hit TATCAACGCAGAGAAAAAAAAAAAA 696 0.8532548731151158 No Hit TNTTTTTTTTTTTTTTTTTTTTTTT 611 0.7490498957950227 No Hit GTACATAAGCAGTGGTATCAACGCA 489 0.5994851048179478 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 456 0.5590290547995587 No Hit TATCAACGCAGAAAAAAAAAAAAAA 447 0.5479955866127252 No Hit ATCAACGCAGAGTACTTTTTTTTTT 374 0.45850190020841 No Hit GTGGTATCAACGCAGAGTACTTTTT 336 0.4119161456417801 No Hit CTTTTTTTTTTTTTTTTTTTTTTTT 320 0.3923010910874096 No Hit AGAAAAAAAAAAAAAAAAAAAAAAA 314 0.38494544562952066 No Hit GAGAAAAAAAAAAAAAAAAAAAAAA 287 0.3518450410690205 No Hit GTGGTATCAACGCAGAAAAAAAAAA 284 0.348167218340076 No Hit ACAAAAAAAAAAAAAAAAAAAAAAA 268 0.32855216378570556 No Hit ACGCAGAGAAAAAAAAAAAAAAAAA 246 0.3015814637734461 No Hit TGTTTTTTTTTTTTTTTTTTTTTTT 238 0.2917739364962609 No Hit TATTTTTTTTTTTTTTTTTTTTTTT 207 0.2537697682971681 No Hit ACGCAGAAAAAAAAAAAAAAAAAAA 201 0.24641412283927916 No Hit GCTTATGTACTCTGCGTTGATACCA 200 0.245188181929631 No Hit GNATCAACGCAGAGTACTTTTTTTT 199 0.24396224101998285 No Hit TCTTTTTTTTTTTTTTTTTTTTTTT 185 0.22679906828490867 No Hit ACTTTTTTTTTTTTTTTTTTTTTTT 182 0.2231212455559642 No Hit GTGGTATCAACGCAGAGAAAAAAAA 180 0.2206693637366679 No Hit ATAAAAAAAAAAAAAAAAAAAAAAA 179 0.21944342282701973 No Hit GTGGTATCAACGCAGAGTACATGGG 175 0.2145396591884271 No Hit GNTATCAACGCAGAAAAAAAAAAAA 156 0.19124678190511218 No Hit TNTCAACGCAGAGTACTTTTTTTTT 150 0.18389113644722324 No Hit AACGCAGAGTACTTTTTTTTTTTTT 149 0.18266519553757507 No Hit GNTATCAACGCAGAGTACTTTTTTT 140 0.1716317273507417 No Hit CTTATGTACTCTGCGTTGATACCAC 140 0.1716317273507417 No Hit GAATCAACGCAGAGTACTTTTTTTT 129 0.15814637734461198 No Hit GTACATGGGAAAAAAAAAAAAAAAA 124 0.1520166727963712 No Hit CAACGCAGAGTACTTTTTTTTTTTT 122 0.1495647909770749 No Hit TCAACGCAGAGTACTTTTTTTTTTT 121 0.14833885006742675 No Hit GTATCAACGCAGAGTAAAAAAAAAA 117 0.14343508642883412 No Hit GGTATCAACGCAGAGTACATGGGGG 113 0.1385313227902415 No Hit GNTATCAACGCAGAGAAAAAAAAAA 113 0.1385313227902415 No Hit GNACTTTTTTTTTTTTTTTTTTTTT 111 0.13607944097094518 No Hit GAGTAAAAAAAAAAAAAAAAAAAAA 110 0.13485350006129704 No Hit GTACATGGGGAAAAAAAAAAAAAAA 109 0.1336275591516489 No Hit GCATCAACGCAGAGTACTTTTTTTT 109 0.1336275591516489 No Hit CCCATGTACTCTGCGTTGATACCAC 106 0.12994973642270444 No Hit TATGTACTCTGCGTTGATACCACTG 102 0.1250459727841118 No Hit GAGTACATAAGCAGTGGTATCAACG 101 0.12382003187446364 No Hit GGATCAACGCAGAGTACTTTTTTTT 100 0.1225940909648155 No Hit GNATCAACGCAGAGAAAAAAAAAAA 96 0.11769032732622288 No Hit GGTATCAACGCAGAGTAAAAAAAAA 95 0.11646438641657471 No Hit GTATCAACGCAGAGTACATGGGGAA 95 0.11646438641657471 No Hit TATCAACGCAGAGTAAAAAAAAAAA 94 0.11523844550692657 No Hit ATCAACGCAGAGAAAAAAAAAAAAA 93 0.11401250459727841 No Hit GCGTTGATACCACTGCTTATGTACT 93 0.11401250459727841 No Hit GGTATCAACGCAGAGTACATGGGGA 90 0.11033468186833395 No Hit ANGCAGAGTACTTTTTTTTTTTTTT 88 0.10788280004903764 No Hit GAGTACATGGGAAAAAAAAAAAAAA 86 0.10543091822974134 No Hit GTATCAACGCAGAGTACATGGGAAA 85 0.10420497732009316 No Hit GNATCAACGCAGAAAAAAAAAAAAA 84 0.10297903641044502 No Hit GGTATCAACGCAGAGTACATGGGAA 84 0.10297903641044502 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTCAAC 25 0.0043656803 20.063192 1 GGTATCA 965 0.0 12.162662 1 ATGGGGA 170 1.7378079E-7 8.885779 5 TGGGGAA 100 0.0030088362 8.497026 6 TACATAA 120 9.6051703E-4 8.359664 2 GTATCAA 1630 0.0 8.246834 1 CGCAGAA 535 0.0 7.941147 9 GCAGAAA 535 0.0 7.941147 10 CAGAAAA 540 0.0 7.867617 11 GGGGAAA 110 0.007097606 7.724569 7 CATAAGC 135 6.505469E-4 7.6927814 4 ACATAAG 125 0.0026177748 7.552912 3 GTACATA 135 0.0030658739 7.430812 1 TGGTATC 330 2.5520421E-9 6.991719 2 GTGGTAT 340 4.7566573E-9 6.78608 1 AGAGAAA 375 4.2018655E-10 6.5458574 12 ATCAACG 2255 0.0 6.531343 3 TATCAAC 2065 0.0 6.5096073 2 ATAAGCA 160 0.0039285426 6.4907837 5 CATGGGG 335 1.240187E-8 6.4819775 4 >>END_MODULE