FastQCFastQC Report
Thu 26 May 2016
SRR1512143_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512143_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81570
Sequences flagged as poor quality0
Sequence length25
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT1021412.521760451146255No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA935911.473580973397082No Hit
GTATCAACGCAGAGTACTTTTTTTT44465.450533284295697No Hit
GGTATCAACGCAGAGTACTTTTTTT31693.8850067426750035No Hit
TATCAACGCAGAGTACTTTTTTTTT31593.8727473335785216No Hit
GGTATCAACGCAGAAAAAAAAAAAA19832.431040823832291No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18952.323158023783254No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17352.1270074782395487No Hit
GGTATCAACGCAGAGAAAAAAAAAA13811.693024396224102No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11971.4674512688488415No Hit
GTATCAACGCAGAGAAAAAAAAAAA11551.4159617506436188No Hit
GTATCAACGCAGAAAAAAAAAAAAA9121.1180581095991173No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA7080.8679661640308938No Hit
TATCAACGCAGAGAAAAAAAAAAAA6890.8446732867475788No Hit
GCAGAGTACTTTTTTTTTTTTTTTT6600.8091210003677823No Hit
GTACATAAGCAGTGGTATCAACGCA5390.6607821503003555No Hit
ATCAACGCAGAGTACTTTTTTTTTT5150.6313595684687998No Hit
GTGGTATCAACGCAGAGTACTTTTT4670.5725144048056884No Hit
TATCAACGCAGAAAAAAAAAAAAAA4260.5222508275101141No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT3720.45605001838911363No Hit
GTGGTATCAACGCAGAAAAAAAAAA3210.39352703199705774No Hit
ACGCAGAGAAAAAAAAAAAAAAAAA2830.34694127743042785No Hit
GCTTATGTACTCTGCGTTGATACCA2680.32855216378570556No Hit
GAGAAAAAAAAAAAAAAAAAAAAAA2610.3199705774181685No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT2400.2942258183155572No Hit
CAACGCAGAGTACTTTTTTTTTTTT2070.2537697682971681No Hit
GTGGTATCAACGCAGAGAAAAAAAA2060.2525438273875199No Hit
ACGCAGAAAAAAAAAAAAAAAAAAA2050.2513178864778718No Hit
AACGCAGAGTACTTTTTTTTTTTTT2000.245188181929631No Hit
GTGGTATCAACGCAGAGTACATGGG1870.229250950104205No Hit
TCAACGCAGAGTACTTTTTTTTTTT1710.2096358955498345No Hit
CTTATGTACTCTGCGTTGATACCAC1650.20228025009194558No Hit
GGTATCAACGCAGAGTACATGGGGG1450.17776143189898247No Hit
GTATCAACGCAGAGTAAAAAAAAAA1360.16672796371214907No Hit
TATGTACTCTGCGTTGATACCACTG1250.15324261370601935No Hit
GAGTACATAAGCAGTGGTATCAACG1160.14220914551918598No Hit
GTACTGGTTCACTATCGGTCAGTCA1140.13975726369988967No Hit
GTACATGGGAAAAAAAAAAAAAAAA1130.1385313227902415No Hit
TATCAACGCAGAGTAAAAAAAAAAA1130.1385313227902415No Hit
GGTATCAACGCAGAGTACATGGGGA1080.13240161824200075No Hit
GTACATGGGGAAAAAAAAAAAAAAA1070.13117567733235258No Hit
CATAAGCAGTGGTATCAACGCAGAG1060.12994973642270444No Hit
ATCAACGCAGAGAAAAAAAAAAAAA1050.12872379551305627No Hit
GAGTAAAAAAAAAAAAAAAAAAAAA990.12136815005516734No Hit
GTATCAACGCAGAGTACATGGGAAA980.1201422091455192No Hit
CCCATGTACTCTGCGTTGATACCAC880.10788280004903764No Hit
GCGTTGATACCACTGCTTATGTACT880.10788280004903764No Hit
TTTTTTTTCTTTTTTTTTTTTTTTT850.10420497732009316No Hit
ACGCAGAGTACATAAGCAGTGGTAT830.10175309550079685No Hit
GTACATGGGGGGTCTTGAGGGGTTT830.10175309550079685No Hit
GGTATCAACGCAGAGTACATGGGAA830.10175309550079685No Hit
GGTATCAACGCAGAGTAAAAAAAAA830.10175309550079685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11900.012.8870551
GCAGAAA4250.011.84208810
CGCAGAA4300.011.7043919
CAGAAAA4300.011.70439111
GTACATA1303.519508E-710.2579241
TACATAA1252.4341862E-69.887982
ACATAAG1401.0288413E-58.8285543
CATAAGC1401.0288413E-58.8285544
ATAAGCA1451.6021319E-58.524125
TAAGCAG1451.6271752E-58.5136686
GTATCAA20750.08.0792191
AAGCAGT2001.5407E-57.12201177
GCAGTGG2052.1783439E-56.9483039
AGAGAAA4450.06.82859511
AGTGGTA2103.0507254E-56.782867411
AGCAGTG2103.0507254E-56.78286748
CAGTGGT2103.0507254E-56.782867410
CAGAGAA4500.06.752721310
TATCAAC25050.06.68004232
GCAGAGA4550.06.67851549
Produced by FastQC (version 0.11.5)