##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512143_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 81570 Sequences flagged as poor quality 0 Sequence length 25 %GC 27 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.060966041436803 31.0 31.0 34.0 28.0 34.0 2 31.157092068162314 33.0 31.0 34.0 28.0 34.0 3 31.04819173715827 33.0 31.0 34.0 27.0 34.0 4 34.80993012136815 37.0 35.0 37.0 32.0 37.0 5 34.54472232438396 37.0 35.0 37.0 32.0 37.0 6 34.60004903763639 37.0 35.0 37.0 31.0 37.0 7 34.48569326958441 37.0 35.0 37.0 31.0 37.0 8 34.46465612357484 37.0 35.0 37.0 31.0 37.0 9 35.84572759592987 39.0 37.0 39.0 30.0 39.0 10 35.82606350373912 39.0 37.0 39.0 30.0 39.0 11 35.74900085815864 39.0 37.0 39.0 30.0 39.0 12 35.63177638837808 39.0 37.0 39.0 30.0 39.0 13 35.454382738751995 39.0 35.0 39.0 30.0 39.0 14 36.39122226308692 40.0 36.0 41.0 28.0 41.0 15 36.228588942013 40.0 36.0 41.0 27.0 41.0 16 36.224506558783865 40.0 36.0 41.0 28.0 41.0 17 35.97454946671571 39.0 36.0 40.0 30.0 41.0 18 35.62697070001226 38.0 35.0 40.0 30.0 41.0 19 35.51135221282334 38.0 35.0 41.0 30.0 41.0 20 35.49390707367905 38.0 35.0 41.0 30.0 41.0 21 35.41442932450656 38.0 35.0 41.0 30.0 41.0 22 35.2442564668383 38.0 35.0 41.0 30.0 41.0 23 34.94191491970087 37.0 35.0 41.0 28.0 41.0 24 34.78530096849332 37.0 35.0 41.0 27.0 41.0 25 34.68942012994974 36.0 35.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.8214807994820497 1101 2 -0.7288244764260874 1101 3 -0.3505633476079453 1101 4 -0.7950161863182714 1101 5 -0.6478272567319436 1101 6 -0.3394959156068964 1101 7 -0.9044470753359235 1101 8 -1.292157228955375 1101 9 -1.2526181943938752 1101 10 -1.0812361906355008 1101 11 -1.591467557972571 1101 12 -1.7321034300931117 1101 13 -0.8857955621976643 1101 14 -0.9340595565746099 1101 15 -1.9024420669417736 1101 16 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-1.5652707800374728 2314 20 -1.5114604136656524 2314 21 -1.2669173370529165 2314 22 -0.8164600681989214 2314 23 -0.5657168192033382 2314 24 -0.9578393716210414 2314 25 -1.344033762877629 2315 1 0.20404003385128533 2315 2 0.25294635690724476 2315 3 0.36454081905872115 2315 4 0.48050464701506 2315 5 0.4688394099347235 2315 6 0.1605040843931036 2315 7 0.33461542466407934 2315 8 0.48961360437795776 2315 9 0.8390484722727933 2315 10 0.5067846426978306 2315 11 0.5621782753607647 2315 12 0.8095632365735526 2315 13 1.1178502711356657 2315 14 0.34250294342538723 2315 15 0.13037043305822493 2315 16 0.899527188730481 2315 17 0.5465619887651698 2315 18 0.7275083770212731 2315 19 0.3554792199625254 2315 20 0.8389770863343458 2315 21 0.2821451629470815 2315 22 0.16447743180108176 2315 23 -0.01984181920333583 2315 24 0.29553562837895697 2315 25 0.38971623712237147 2316 1 0.015906587905337943 2316 2 -0.4090046566062675 2316 3 -0.4663291133737104 2316 4 -0.004926096228182075 2316 5 -0.3825119414166309 2316 6 -0.5084148345258157 2316 7 -0.2990416699305172 2316 8 -0.48990497670312294 2316 9 -0.34676233853802074 2316 10 -0.27763258703189564 2316 11 -0.15678287328788088 2316 12 0.15753620954652803 2316 13 -0.6011108775129799 2316 14 -0.35027577279083033 2316 15 -0.9078474723471786 2316 16 -0.6845099734316804 2316 17 -0.4063518625861846 2316 18 -0.15319263649224268 2316 19 0.1367292199625254 2316 20 0.05540445119920889 2316 21 -0.0677281478637326 2316 22 0.06164803990918699 2316 23 -0.29982492731144106 2316 24 -0.3003258581075272 2316 25 -0.6905202493641127 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1314.0 3 0.0 4 23.0 5 220.0 6 254.0 7 190.0 8 168.0 9 132.0 10 116.0 11 127.0 12 119.0 13 124.0 14 93.0 15 98.0 16 127.0 17 114.0 18 125.0 19 165.0 20 178.0 21 206.0 22 282.0 23 343.0 24 371.0 25 497.0 26 550.0 27 672.0 28 839.0 29 977.0 30 1303.0 31 1619.0 32 2120.0 33 2975.0 34 3894.0 35 5730.0 36 16985.0 37 16841.0 38 21679.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.02906940937365 23.393484017983617 23.889881135677772 8.687565436964958 2 14.902537697682972 29.925217604511463 41.84994483265906 13.3222998651465 3 15.06313595684688 36.87017285766826 39.040088267745496 9.026602917739366 4 7.811695476278044 29.800171631727352 37.75407625352458 24.634056638470025 5 5.397911989498608 41.69641652251788 38.46380331971587 14.441868168267638 6 15.427240407012382 42.08042172367292 25.08275101140125 17.409586857913446 7 11.829103837195047 47.38384209881084 27.604511462547503 13.18254260144661 8 19.44120928293837 36.11421005529061 26.53336439088379 17.911216270887223 9 21.4306730415594 22.834375383106533 27.12884638960402 28.606105185730048 10 17.79821012627191 34.637734461198974 29.22397940419272 18.340076008336396 11 18.660046585754568 34.06031629275469 26.76964570307711 20.50999141841363 12 21.1303175186956 39.90192472722815 30.951330145886967 8.016427608189284 13 25.532671325242124 32.62719136937599 32.184626700993014 9.65551060438887 14 17.696457030771118 37.81782518082629 35.01042049773201 9.47529729067059 15 18.69805075395366 36.49626088022557 36.542846634792205 8.262841731028564 16 9.159004535981365 42.0571288463896 36.480323648400145 12.303542969228882 17 9.242368517837441 40.75272771852397 36.04021086183646 13.964692901802135 18 7.7859507171754325 35.60500183891136 44.63773446119897 11.971312982714233 19 8.977565281353439 37.430427853377466 45.83915655265417 7.752850312614933 20 9.62853990437661 35.57680519798946 46.66176290302808 8.13289199460586 21 10.41804585019002 36.401863430182665 45.64055412529116 7.539536594336154 22 9.531690572514405 36.25107269829594 45.80605614809366 8.41118058109599 23 9.62853990437661 35.988721343631234 46.32462915287483 8.058109599117323 24 8.589152608866222 36.81958611220087 45.68826010200078 8.903001176932131 25 8.766810920815505 37.77415439689097 46.07387610489279 7.385158577400729 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 19773.0 1 19773.0 2 11019.5 3 2266.0 4 2266.0 5 2266.0 6 2436.5 7 2607.0 8 2607.0 9 2607.0 10 2004.0 11 1401.0 12 1401.0 13 1401.0 14 961.0 15 521.0 16 521.0 17 521.0 18 1319.5 19 2118.0 20 2118.0 21 2118.0 22 3479.0 23 4840.0 24 4840.0 25 4840.0 26 6810.0 27 8780.0 28 8780.0 29 8780.0 30 8450.5 31 8121.0 32 8121.0 33 8121.0 34 6824.5 35 5528.0 36 5528.0 37 5528.0 38 4396.0 39 3264.0 40 3264.0 41 3264.0 42 4188.0 43 5112.0 44 5112.0 45 5112.0 46 4921.0 47 4730.0 48 4730.0 49 4730.0 50 4545.0 51 4360.0 52 4360.0 53 4360.0 54 3735.5 55 3111.0 56 3111.0 57 3111.0 58 2562.0 59 2013.0 60 2013.0 61 2013.0 62 1806.0 63 1599.0 64 1599.0 65 1599.0 66 1171.5 67 744.0 68 744.0 69 744.0 70 592.5 71 441.0 72 441.0 73 441.0 74 307.0 75 173.0 76 173.0 77 173.0 78 109.0 79 45.0 80 45.0 81 45.0 82 31.0 83 17.0 84 17.0 85 17.0 86 11.5 87 6.0 88 6.0 89 6.0 90 3.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.47198725021453963 2 0.0 3 0.0 4 0.0 5 0.06987863184994483 6 0.0 7 0.0 8 0.001225940909648155 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.00245188181929631 25 0.001225940909648155 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 81570.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.273262228760576 #Duplication Level Percentage of deduplicated Percentage of total 1 83.46502317741256 19.425033713375015 2 8.680994521702488 4.0407012382003185 3 2.681205225453013 1.8720117690327327 4 1.1957437842393595 1.1131543459605246 5 0.6373788453434471 0.7416942503371338 6 0.4530130636325327 0.6325855093784479 7 0.37399915718499793 0.609292632095133 8 0.31605562579013907 0.5884516366311144 9 0.20543615676359037 0.4303052592865024 >10 1.5539401601348504 7.160720853254873 >50 0.21070375052675938 3.33578521515263 >100 0.1369574378423936 6.457030771116832 >500 0.03160556257901391 4.931960279514527 >1k 0.04740834386852086 24.66593110212088 >5k 0.005267593763168985 11.473580973397082 >10k+ 0.005267593763168985 12.521760451146255 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTT 10214 12.521760451146255 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 9359 11.473580973397082 No Hit GTATCAACGCAGAGTACTTTTTTTT 4446 5.450533284295697 No Hit GGTATCAACGCAGAGTACTTTTTTT 3169 3.8850067426750035 No Hit TATCAACGCAGAGTACTTTTTTTTT 3159 3.8727473335785216 No Hit GGTATCAACGCAGAAAAAAAAAAAA 1983 2.431040823832291 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1895 2.323158023783254 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1735 2.1270074782395487 No Hit GGTATCAACGCAGAGAAAAAAAAAA 1381 1.693024396224102 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1197 1.4674512688488415 No Hit GTATCAACGCAGAGAAAAAAAAAAA 1155 1.4159617506436188 No Hit GTATCAACGCAGAAAAAAAAAAAAA 912 1.1180581095991173 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 708 0.8679661640308938 No Hit TATCAACGCAGAGAAAAAAAAAAAA 689 0.8446732867475788 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 660 0.8091210003677823 No Hit GTACATAAGCAGTGGTATCAACGCA 539 0.6607821503003555 No Hit ATCAACGCAGAGTACTTTTTTTTTT 515 0.6313595684687998 No Hit GTGGTATCAACGCAGAGTACTTTTT 467 0.5725144048056884 No Hit TATCAACGCAGAAAAAAAAAAAAAA 426 0.5222508275101141 No Hit CTTTTTTTTTTTTTTTTTTTTTTTT 372 0.45605001838911363 No Hit GTGGTATCAACGCAGAAAAAAAAAA 321 0.39352703199705774 No Hit ACGCAGAGAAAAAAAAAAAAAAAAA 283 0.34694127743042785 No Hit GCTTATGTACTCTGCGTTGATACCA 268 0.32855216378570556 No Hit GAGAAAAAAAAAAAAAAAAAAAAAA 261 0.3199705774181685 No Hit ACTTTTTTTTTTTTTTTTTTTTTTT 240 0.2942258183155572 No Hit CAACGCAGAGTACTTTTTTTTTTTT 207 0.2537697682971681 No Hit GTGGTATCAACGCAGAGAAAAAAAA 206 0.2525438273875199 No Hit ACGCAGAAAAAAAAAAAAAAAAAAA 205 0.2513178864778718 No Hit AACGCAGAGTACTTTTTTTTTTTTT 200 0.245188181929631 No Hit GTGGTATCAACGCAGAGTACATGGG 187 0.229250950104205 No Hit TCAACGCAGAGTACTTTTTTTTTTT 171 0.2096358955498345 No Hit CTTATGTACTCTGCGTTGATACCAC 165 0.20228025009194558 No Hit GGTATCAACGCAGAGTACATGGGGG 145 0.17776143189898247 No Hit GTATCAACGCAGAGTAAAAAAAAAA 136 0.16672796371214907 No Hit TATGTACTCTGCGTTGATACCACTG 125 0.15324261370601935 No Hit GAGTACATAAGCAGTGGTATCAACG 116 0.14220914551918598 No Hit GTACTGGTTCACTATCGGTCAGTCA 114 0.13975726369988967 No Hit GTACATGGGAAAAAAAAAAAAAAAA 113 0.1385313227902415 No Hit TATCAACGCAGAGTAAAAAAAAAAA 113 0.1385313227902415 No Hit GGTATCAACGCAGAGTACATGGGGA 108 0.13240161824200075 No Hit GTACATGGGGAAAAAAAAAAAAAAA 107 0.13117567733235258 No Hit CATAAGCAGTGGTATCAACGCAGAG 106 0.12994973642270444 No Hit ATCAACGCAGAGAAAAAAAAAAAAA 105 0.12872379551305627 No Hit GAGTAAAAAAAAAAAAAAAAAAAAA 99 0.12136815005516734 No Hit GTATCAACGCAGAGTACATGGGAAA 98 0.1201422091455192 No Hit CCCATGTACTCTGCGTTGATACCAC 88 0.10788280004903764 No Hit GCGTTGATACCACTGCTTATGTACT 88 0.10788280004903764 No Hit TTTTTTTTCTTTTTTTTTTTTTTTT 85 0.10420497732009316 No Hit ACGCAGAGTACATAAGCAGTGGTAT 83 0.10175309550079685 No Hit GTACATGGGGGGTCTTGAGGGGTTT 83 0.10175309550079685 No Hit GGTATCAACGCAGAGTACATGGGAA 83 0.10175309550079685 No Hit GGTATCAACGCAGAGTAAAAAAAAA 83 0.10175309550079685 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1190 0.0 12.887055 1 GCAGAAA 425 0.0 11.842088 10 CGCAGAA 430 0.0 11.704391 9 CAGAAAA 430 0.0 11.704391 11 GTACATA 130 3.519508E-7 10.257924 1 TACATAA 125 2.4341862E-6 9.88798 2 ACATAAG 140 1.0288413E-5 8.828554 3 CATAAGC 140 1.0288413E-5 8.828554 4 ATAAGCA 145 1.6021319E-5 8.52412 5 TAAGCAG 145 1.6271752E-5 8.513668 6 GTATCAA 2075 0.0 8.079219 1 AAGCAGT 200 1.5407E-5 7.1220117 7 GCAGTGG 205 2.1783439E-5 6.948303 9 AGAGAAA 445 0.0 6.828595 11 AGTGGTA 210 3.0507254E-5 6.7828674 11 AGCAGTG 210 3.0507254E-5 6.7828674 8 CAGTGGT 210 3.0507254E-5 6.7828674 10 CAGAGAA 450 0.0 6.7527213 10 TATCAAC 2505 0.0 6.6800423 2 GCAGAGA 455 0.0 6.6785154 9 >>END_MODULE