FastQCFastQC Report
Thu 26 May 2016
SRR1512142_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512142_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2625166
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51140.19480672841260324No Hit
TATCAACGCAGAGTACTTTTTTTTT34060.12974417617781123No Hit
GGTATCAACGCAGAGTACTTTTTTT32890.1252873151640696No Hit
GTCCTACAGTGGACATTTCTAAATT30430.11591647918645907No Hit
GTCCTAAAGTGTGTATTTCTCATTT29920.11397374489841784No Hit
CTTTAGGACGTGAAATATGGCGAGG28190.10738368545074864No Hit
CTGTAGGACGTGGAATATGGCAAGA27630.10525048701682103No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT400.00400372614.8563711
GGTATCA12850.012.5633651
AACCGTC658.3562493E-411.636347
TAGGACC5800.011.4106354
GTACTAA1801.6370905E-1111.0047211
CGGTAGG1001.5140828E-510.8946731
GTCCTAG2950.010.7435911
GTACCGT803.9435216E-410.6363426
TTGGACG909.994288E-510.505034
TCCAACG2100.010.35515418
GGCGAGG8900.010.30456719
GTATCAA25650.010.2710711
GTATAGG2700.010.2710711
ACCGTCC1201.6215054E-610.2424038
CCTATAC1856.548362E-1110.2211093
ACCGTCT951.7263726E-49.9521338
ATAGGAC3050.09.9195033
TTAGGAC12400.09.6832653
GTAGGAC20850.09.6585813
TAGGACA7050.09.6556864
Produced by FastQC (version 0.11.5)