FastQCFastQC Report
Thu 26 May 2016
SRR1512142_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512142_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2625166
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66110.25183169369098946No Hit
TATCAACGCAGAGTACTTTTTTTTT45250.17237005202718608No Hit
GGTATCAACGCAGAGTACTTTTTTT44320.16882741891369918No Hit
GTCCTACAGTGGACATTTCTAAATT35430.1349628937750984No Hit
GTCCTAAAGTGTGTATTTCTCATTT33370.127115770964579No Hit
CTTTAGGACGTGAAATATGGCGAGG33030.12582061477255152No Hit
CTGTAGGACGTGGAATATGGCAAGA31700.12075426849197347No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGG400.005294311314.24434410
TCCAACG1800.013.18970918
TAGGACC6000.012.9863414
GGTATCA17750.012.9011561
CCGTGCG604.1074282E-412.6616399
ACCGATC701.0951158E-412.2099038
CTAGTAC1104.957692E-711.2298753
TGACCGC600.00586735811.0859025
CCAACGT951.3664985E-510.99605319
GCGTGCG1002.4106394E-510.4458529
GTATTAG2850.010.0436631
GTACTAG2002.3646862E-1110.0185551
GAGCCGT1151.0313926E-59.9094866
GGCGAGG8700.09.71490319
AGGACCT13900.09.6389245
CGTACAC1106.822193E-59.5022013
GACGTGA10850.09.3653017
GTTTAAG2550.09.3543921
TGGACCG1356.830458E-69.1502685
GACCGAG1356.881821E-69.1448657
Produced by FastQC (version 0.11.5)