FastQCFastQC Report
Thu 26 May 2016
SRR1512141_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512141_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2409936
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56920.23618884484899183No Hit
GTCCTAAAGTGTGTATTTCTCATTT38510.15979677468613274No Hit
GTCCTACAGTGGACATTTCTAAATT37400.15519084324231017No Hit
TATCAACGCAGAGTACTTTTTTTTT37290.15473439958571514No Hit
GGTATCAACGCAGAGTACTTTTTTT37230.1544854303184815No Hit
CTGTAGGACGTGGAATATGGCAAGA36990.15348955324954688No Hit
CTTTAGGACGTGAAATATGGCGAGG36770.1525766659363568No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACG500.001552901113.2389144
CGGTAGG759.799092E-613.1837281
TAGGACC7600.012.1937374
GGTATCA14750.011.5972131
GGCGAGG12250.011.34764119
ACACCGT803.9361845E-410.6384136
CTAGGAC3000.010.4020033
GTCCTAC20800.010.1730221
CCGTGCG750.002751546710.0867919
GTCCTAA15350.010.0488361
AGGACGT26000.010.0382975
GACGTGG12350.010.0306417
GGACGTG25300.010.0170226
TGGCGAG25400.09.97758518
CGTGCGC951.7228535E-49.95407110
GTCAACG800.00324531239.8877962
CTTACTC1356.8649024E-79.8066033
CTTAGAC3100.09.7614123
GTAGGAC24300.09.5731133
TCCTACA22600.09.53778652
Produced by FastQC (version 0.11.5)