FastQCFastQC Report
Thu 26 May 2016
SRR1512141_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512141_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2409936
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT70600.29295383777826467No Hit
TATCAACGCAGAGTACTTTTTTTTT49410.20502619156691299No Hit
GGTATCAACGCAGAGTACTTTTTTT47790.19830402135160435No Hit
CTTTAGGACGTGAAATATGGCGAGG41100.17054394805505207No Hit
GTCCTAAAGTGTGTATTTCTCATTT39570.16419523174059394No Hit
CTGTAGGACGTGGAATATGGCAAGA38820.1610831159001733No Hit
GTCCTACAGTGGACATTTCTAAATT37640.15618672031124478No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1650.012.66240618
GGTATCA17750.012.5213051
GAGCGTC701.0948133E-412.2101787
CTACGTT550.003064621712.0933664
TAGGACC6450.012.0799744
TTAGGAC14400.011.1515713
CCAACGT1303.2840035E-810.95807819
GGCGAGG11100.010.95149719
AGTCGTC2200.010.7918238
GACGTGA12400.010.4158517
GGACGTG21800.010.2809456
CTATACC2151.8189894E-1210.1648574
AGGACGT21900.010.1527475
AAGTCGT2250.010.1299267
TAGAGTG2350.010.1084385
TTTAGGA17150.010.0282762
CAAGTCG2002.5465852E-119.9716456
TGGCGAG24550.09.74824718
ACGTGAA14500.09.6932918
CGTGAAA14750.09.5287999
Produced by FastQC (version 0.11.5)