##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512141_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2409936 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.505637494107727 31.0 31.0 34.0 27.0 34.0 2 30.70973461535908 31.0 31.0 34.0 27.0 34.0 3 30.80295493324304 31.0 31.0 34.0 27.0 34.0 4 34.40707512564649 37.0 35.0 37.0 30.0 37.0 5 33.98296635263343 37.0 35.0 37.0 30.0 37.0 6 33.913902277902814 37.0 35.0 37.0 30.0 37.0 7 33.723914659974376 37.0 35.0 37.0 29.0 37.0 8 33.61119465413189 37.0 35.0 37.0 28.0 37.0 9 35.056683662968645 38.0 35.0 39.0 28.0 39.0 10 34.83525786576905 38.0 35.0 39.0 28.0 39.0 11 34.82340526885361 38.0 35.0 39.0 27.0 39.0 12 34.63864476069074 38.0 35.0 39.0 27.0 39.0 13 34.60890745646358 38.0 35.0 39.0 27.0 39.0 14 35.543022719275534 39.0 35.0 41.0 27.0 41.0 15 35.48114099295583 39.0 35.0 41.0 27.0 41.0 16 35.370950929817226 39.0 35.0 41.0 27.0 41.0 17 35.31092319464086 39.0 35.0 40.0 27.0 41.0 18 35.22818738754888 39.0 35.0 40.0 27.0 41.0 19 35.24820410168569 39.0 35.0 40.0 27.0 41.0 20 35.12807269570644 39.0 34.0 40.0 26.0 41.0 21 34.99463097775211 39.0 34.0 40.0 25.0 41.0 22 34.867031738602186 39.0 34.0 40.0 25.0 41.0 23 34.92316144495123 39.0 34.0 40.0 25.0 41.0 24 34.800331627063954 39.0 34.0 40.0 25.0 41.0 25 34.35194627575172 38.0 34.0 40.0 23.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07728847752634849 1101 2 0.08177002940179179 1101 3 0.13177864824537622 1101 4 0.09530558696251745 1101 5 0.2394215162697222 1101 6 -0.06444048077179332 1101 7 0.018212923891418598 1101 8 -0.025925344506212866 1101 9 0.05235079867603076 1101 10 0.0955863822430274 1101 11 -0.02253104081545132 1101 12 0.01871683952313674 1101 13 0.0467124786667128 1101 14 0.07671911476651871 1101 15 0.11982545256807242 1101 16 -0.041426634744567536 1101 17 0.021600469016433976 1101 18 0.1696461457112477 1101 19 -0.15054441620498693 1101 20 -0.026320676864820314 1101 21 -0.07771137744207124 1101 22 -0.20282614050919534 1101 23 -0.15479615175977557 1101 24 -0.36400007995772654 1101 25 -0.29828087068141684 1102 1 0.38971774176121343 1102 2 0.37299129653399277 1102 3 0.43912059625175814 1102 4 0.5620894863706027 1102 5 0.15986783065341825 1102 6 0.41566579264174663 1102 7 0.45564713962983916 1102 8 0.4698050447102631 1102 9 0.5944718247407437 1102 10 0.6751174324144884 1102 11 0.678893294551294 1102 12 0.6961546769327356 1102 13 0.7514090505285864 1102 14 0.8868282459049652 1102 15 0.6867605850420162 1102 16 0.7013462921316318 1102 17 0.9191191998470458 1102 18 0.8114381089965548 1102 19 0.7410254367777966 1102 20 1.017109668212541 1102 21 0.9162789119520909 1102 22 1.0940101316593953 1102 23 1.021623797989541 1102 24 0.8237269818483526 1102 25 0.8124312121190442 1103 1 0.26770617861395607 1103 2 0.33987099583409375 1103 3 0.37620510829883713 1103 4 0.33042799083519725 1103 5 0.38520920827596683 1103 6 0.385996772695556 1103 7 0.3589756751488764 1103 8 0.41334016277520647 1103 9 0.4317255046781341 1103 10 0.23846206204257925 1103 11 0.25025974524718464 1103 12 0.39712122220225865 1103 13 0.3027148994375324 1103 14 0.3992197765874721 1103 15 0.48198499692953334 1103 16 0.37127032212972466 1103 17 0.40323266193325225 1103 18 0.4412696255206754 1103 19 0.45231367757000385 1103 20 0.4225045822528486 1103 21 0.6425539276452312 1103 22 0.5280203093859228 1103 23 0.3474076599120366 1103 24 0.7142780529915029 1103 25 0.4248879350044774 1104 1 0.17255907119616865 1104 2 0.17700236039205564 1104 3 0.19615456307126067 1104 4 0.16964092943437237 1104 5 0.19383188142091257 1104 6 0.12319065586632405 1104 7 0.059186688215568495 1104 8 0.17535026366168438 1104 9 0.2706421682851641 1104 10 0.2129992407295518 1104 11 0.33659203422391926 1104 12 0.4417302412005739 1104 13 0.28075485423647706 1104 14 0.44455494978340937 1104 15 0.44524536808407333 1104 16 0.5916892274318712 1104 17 0.5621533438654751 1104 18 0.5667056240030917 1104 19 0.6182307328107584 1104 20 0.6204055682723464 1104 21 0.625636676587618 1104 22 0.4897687091950047 1104 23 0.5598087825953826 1104 24 0.3519016113918454 1104 25 0.6321631433658581 1105 1 -0.23206524011934349 1105 2 -0.1573357995902711 1105 3 -0.17513825586580722 1105 4 -0.11684478810290244 1105 5 -0.03992286322191063 1105 6 -0.24448368256143738 1105 7 0.025529179462665752 1105 8 -0.05417497080902223 1105 9 -0.12990961610220353 1105 10 -0.1824397098592243 1105 11 -0.07201744234239271 1105 12 -0.26887282214836006 1105 13 -0.15492084315070542 1105 14 -0.06554201833125006 1105 15 0.08957941102183753 1105 16 0.016675838669058862 1105 17 0.09768812452406905 1105 18 -0.07761620168860617 1105 19 -0.19947155465936817 1105 20 -0.04998434597263213 1105 21 -0.028942782372482156 1105 22 -0.22946023195468968 1105 23 -0.21441684483551882 1105 24 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-0.6785594457885615 2315 3 -0.7145452569107391 2315 4 -0.7937779124498192 2315 5 -0.8633500867759096 2315 6 -0.8276456777670376 2315 7 -0.8266743978930364 2315 8 -0.9692304444091207 2315 9 -1.2159689928309447 2315 10 -0.9943964988444094 2315 11 -0.8365327782573289 2315 12 -0.9185570375273002 2315 13 -0.9619019114400942 2315 14 -1.0547062263342042 2315 15 -1.1664858050332327 2315 16 -1.1900839498858602 2315 17 -1.272860157484665 2315 18 -1.2409413906983815 2315 19 -1.1060154418067043 2315 20 -1.1777042427087565 2315 21 -1.0883072178018267 2315 22 -1.2135503727131862 2315 23 -1.127244922775155 2315 24 -1.1749510738766844 2315 25 -1.2890864815966339 2316 1 -1.1699866656128926 2316 2 -1.2289382336673498 2316 3 -1.246704656233348 2316 4 -1.2900191927584572 2316 5 -1.5474177246647898 2316 6 -1.5909884878067402 2316 7 -1.6782388955715248 2316 8 -1.6578334433100466 2316 9 -1.755030967126082 2316 10 -2.085925220554479 2316 11 -1.9768687807694931 2316 12 -1.9767218649278746 2316 13 -1.9577630019903012 2316 14 -2.1482051496906394 2316 15 -2.050504937886565 2316 16 -2.3202226798950534 2316 17 -2.258811080482218 2316 18 -2.198828645930192 2316 19 -2.117302535541988 2316 20 -2.189609565365373 2316 21 -2.3136464726747548 2316 22 -2.383586316907163 2316 23 -2.3031707240895543 2316 24 -2.373664709118813 2316 25 -2.4874799275289448 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 45671.0 3 1.0 4 797.0 5 7348.0 6 9077.0 7 7134.0 8 5820.0 9 5319.0 10 4889.0 11 4642.0 12 4533.0 13 4287.0 14 4214.0 15 4154.0 16 4378.0 17 4473.0 18 4914.0 19 5582.0 20 6890.0 21 8773.0 22 11283.0 23 14323.0 24 17510.0 25 20828.0 26 23705.0 27 27547.0 28 32002.0 29 39199.0 30 49373.0 31 63761.0 32 81586.0 33 104915.0 34 135373.0 35 178331.0 36 257557.0 37 437039.0 38 772708.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.692249379144336 20.705635439887544 14.053655098893067 24.548460082075056 2 14.732009480749698 21.79833821313097 40.488253629971915 22.981398676147418 3 18.14438225745414 26.099074830202962 31.053355773763286 24.70318713857961 4 12.778181661255736 18.47791808579149 38.70019784757769 30.04370240537508 5 12.8202605220821 38.36541595308527 36.205054751154705 12.609268773677929 6 30.46823650088633 38.42052237071856 18.465594107063424 12.645647021331689 7 26.79494393212102 32.41915967892923 23.325889152243047 17.4600072367067 8 24.518135308443615 37.47504519977741 21.205822586153452 16.80099690562552 9 26.178620511084112 15.443065707969009 20.984416183666287 37.39389759728059 10 14.935998300369802 29.530991694385243 34.82200357187909 20.711006433365867 11 33.31042816074783 22.84471454843614 25.412376096294675 18.43248119452135 12 22.748114472749485 25.262953041076607 32.361232829419535 19.627699656754373 13 28.48063185080434 22.549976430909368 26.322441757789417 22.646949960496876 14 20.612331613785596 22.741848746190772 28.278634785322097 28.367184854701538 15 22.512880010091553 31.413074870038045 23.930345038208483 22.14370008166192 16 21.60393471029936 28.600759522244573 28.330420392906703 21.464885374549368 17 21.232223594319517 29.08807536797658 28.36801475225898 21.311686285444924 18 20.999520319211797 27.928210541690735 30.443339574162962 20.628929564934502 19 22.970504586432657 27.348176340098938 28.286293317576334 21.395025755892068 20 23.090571699829372 27.7863810491233 28.712920177133334 20.410127073913998 21 22.531262240988973 27.20088832234549 27.928915954614563 22.338933482050976 22 22.755957004667344 28.286186853094854 28.131908897165737 20.82594724507207 23 21.862240325054273 28.127344460599783 28.4374356829393 21.572979531406645 24 22.5637290906752 27.385312535013135 28.92655437565617 21.124403998655495 25 22.261596708256082 27.77139210293505 28.660098227816167 21.3069129609927 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 916.0 1 916.0 2 895.5 3 875.0 4 875.0 5 875.0 6 2060.5 7 3246.0 8 3246.0 9 3246.0 10 4715.5 11 6185.0 12 6185.0 13 6185.0 14 10262.0 15 14339.0 16 14339.0 17 14339.0 18 24745.0 19 35151.0 20 35151.0 21 35151.0 22 53808.0 23 72465.0 24 72465.0 25 72465.0 26 102476.5 27 132488.0 28 132488.0 29 132488.0 30 167627.0 31 202766.0 32 202766.0 33 202766.0 34 226003.0 35 249240.0 36 249240.0 37 249240.0 38 265182.0 39 281124.0 40 281124.0 41 281124.0 42 292713.5 43 304303.0 44 304303.0 45 304303.0 46 304360.5 47 304418.0 48 304418.0 49 304418.0 50 290528.5 51 276639.0 52 276639.0 53 276639.0 54 243903.0 55 211167.0 56 211167.0 57 211167.0 58 178783.0 59 146399.0 60 146399.0 61 146399.0 62 117674.0 63 88949.0 64 88949.0 65 88949.0 66 68112.5 67 47276.0 68 47276.0 69 47276.0 70 33909.0 71 20542.0 72 20542.0 73 20542.0 74 14209.0 75 7876.0 76 7876.0 77 7876.0 78 5205.5 79 2535.0 80 2535.0 81 2535.0 82 1655.0 83 775.0 84 775.0 85 775.0 86 491.5 87 208.0 88 208.0 89 208.0 90 126.5 91 45.0 92 45.0 93 45.0 94 26.5 95 8.0 96 8.0 97 8.0 98 4.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.43258410181847157 2 0.0 3 0.0 4 0.0 5 0.05439978489055311 6 0.0 7 0.0 8 0.0032780953519097604 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 8.29897557445509E-5 20 0.0 21 0.0 22 0.0 23 0.0 24 0.005228354611906706 25 0.0011618565804237125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2409936.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.3522018522274 #Duplication Level Percentage of deduplicated Percentage of total 1 81.74087975292285 57.50650871956275 2 12.358709122486545 17.389247976362746 3 3.2446541559506152 6.848056923603185 4 1.1701946678575086 3.2930308591804653 5 0.5343154585475084 1.8795134496249877 6 0.2701917930952027 1.1405152539989376 7 0.1604517536617406 0.7901693912808249 8 0.09768969210598753 0.5498147950337903 9 0.06888249670194137 0.43614317808543296 >10 0.3182900613273408 3.778753147859315 >50 0.01793633656124704 0.8727577154221474 >100 0.01427259196925803 2.0467860408556957 >500 0.002119270087324104 1.1004476852818228 >1k 0.0013539781113407969 2.0758623811385006 >5k 5.886861353655639E-5 0.2923924827092914 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7060 0.29295383777826467 No Hit TATCAACGCAGAGTACTTTTTTTTT 4941 0.20502619156691299 No Hit GGTATCAACGCAGAGTACTTTTTTT 4779 0.19830402135160435 No Hit CTTTAGGACGTGAAATATGGCGAGG 4110 0.17054394805505207 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3957 0.16419523174059394 No Hit CTGTAGGACGTGGAATATGGCAAGA 3882 0.1610831159001733 No Hit GTCCTACAGTGGACATTTCTAAATT 3764 0.15618672031124478 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 4.149487787227545E-5 0.0 0.0 0.0 3 0.0 4.149487787227545E-5 0.0 4.149487787227545E-5 0.0 4 0.0 4.149487787227545E-5 0.0 4.149487787227545E-5 0.0 5 0.0 8.29897557445509E-5 0.0 4.149487787227545E-5 0.0 6 0.0 8.29897557445509E-5 0.0 4.149487787227545E-5 0.0 7 0.0 8.29897557445509E-5 0.0 4.149487787227545E-5 0.0 8 0.0 8.29897557445509E-5 0.0 4.149487787227545E-5 0.0 9 0.0 8.29897557445509E-5 0.0 4.149487787227545E-5 0.0 10 0.0 2.0747438936137723E-4 0.0 4.149487787227545E-5 0.0 11 0.0 2.0747438936137723E-4 0.0 4.149487787227545E-5 0.0 12 0.0 2.0747438936137723E-4 0.0 8.29897557445509E-5 0.0 13 0.0 2.9046414510592813E-4 0.0 8.29897557445509E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 165 0.0 12.662406 18 GGTATCA 1775 0.0 12.521305 1 GAGCGTC 70 1.0948133E-4 12.210178 7 CTACGTT 55 0.0030646217 12.093366 4 TAGGACC 645 0.0 12.079974 4 TTAGGAC 1440 0.0 11.151571 3 CCAACGT 130 3.2840035E-8 10.958078 19 GGCGAGG 1110 0.0 10.951497 19 AGTCGTC 220 0.0 10.791823 8 GACGTGA 1240 0.0 10.415851 7 GGACGTG 2180 0.0 10.280945 6 CTATACC 215 1.8189894E-12 10.164857 4 AGGACGT 2190 0.0 10.152747 5 AAGTCGT 225 0.0 10.129926 7 TAGAGTG 235 0.0 10.108438 5 TTTAGGA 1715 0.0 10.028276 2 CAAGTCG 200 2.5465852E-11 9.971645 6 TGGCGAG 2455 0.0 9.748247 18 ACGTGAA 1450 0.0 9.693291 8 CGTGAAA 1475 0.0 9.528799 9 >>END_MODULE