FastQCFastQC Report
Thu 26 May 2016
SRR1512140_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512140_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3169274
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT89410.28211508376997385No Hit
TATCAACGCAGAGTACTTTTTTTTT57890.1826601297331818No Hit
GGTATCAACGCAGAGTACTTTTTTT56400.1779587375531431No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18700.012.1068541
CCAACGT1604.9112714E-1111.22961719
TAGGACC5650.011.2139264
AATCCCG3300.011.17588719
TACACCG1252.388806E-710.5913025
TGTGCGA750.00275158810.08695410
GTATCAA35400.010.0539481
AGTACCG1606.662958E-910.0475525
TCCAACG3050.09.92159518
TAGGACT4300.09.8963584
TGGCGAG13950.09.82935718
GGACGTG14800.09.7759966
GGCGTGC1554.3670298E-89.7615688
GTAGGAC15000.09.645653
AGGACGT15900.09.5754695
CTAGGAC4350.09.5652163
TTAGGAC11400.09.5394713
ATAGGAC3600.09.456523
TAGGACG16900.09.456524
GACGTGG8000.09.4565197
Produced by FastQC (version 0.11.5)