##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512140_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3169274 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.83303147660947 31.0 28.0 33.0 18.0 34.0 2 26.292574261487015 31.0 25.0 33.0 10.0 34.0 3 27.95450472253267 31.0 27.0 34.0 16.0 34.0 4 32.227640147238766 35.0 32.0 37.0 19.0 37.0 5 32.620043896488596 35.0 33.0 37.0 25.0 37.0 6 32.86580585963851 35.0 33.0 37.0 26.0 37.0 7 32.905172604198945 36.0 35.0 37.0 26.0 37.0 8 32.942106930483135 37.0 35.0 37.0 26.0 37.0 9 34.4682056521462 39.0 35.0 39.0 27.0 39.0 10 34.31921190783757 38.0 35.0 39.0 26.0 39.0 11 34.3387271028002 38.0 35.0 39.0 27.0 39.0 12 34.2224528393569 38.0 35.0 39.0 26.0 39.0 13 34.20476109039483 38.0 35.0 39.0 26.0 39.0 14 35.24468442930463 39.0 35.0 41.0 25.0 41.0 15 35.165289905511486 39.0 35.0 41.0 25.0 41.0 16 35.125662848967934 39.0 35.0 41.0 25.0 41.0 17 35.05978656310562 39.0 35.0 41.0 25.0 41.0 18 35.00188560534684 39.0 35.0 41.0 25.0 41.0 19 35.02275063626559 39.0 35.0 41.0 25.0 41.0 20 34.94439830699397 39.0 35.0 41.0 24.0 41.0 21 34.872805569982276 39.0 35.0 41.0 24.0 41.0 22 34.74967516219803 39.0 34.0 41.0 23.0 41.0 23 34.625801050966245 39.0 34.0 41.0 22.0 41.0 24 34.49660142985428 39.0 34.0 41.0 19.0 41.0 25 34.35597521703709 39.0 34.0 41.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.788849030786757 1101 2 3.15766055415736 1101 3 1.6079961583444522 1101 4 1.007191461039227 1101 5 0.6582668574241382 1101 6 0.4309118257346398 1101 7 0.2263640041420203 1101 8 0.14550447465087046 1101 9 0.05547499180887172 1101 10 -0.16682809648273889 1101 11 0.094340308049631 1101 12 -0.09337079208575005 1101 13 -0.059340949458196235 1101 14 0.08627726758156484 1101 15 0.2513361500587692 1101 16 0.20045820773512446 1101 17 0.11197816459420551 1101 18 -0.016921511241932308 1101 19 0.17127506613901033 1101 20 0.04212142754861503 1101 21 0.05037672481807931 1101 22 -0.18304039298440244 1101 23 0.12310463048386566 1101 24 -0.011786800353377203 1101 25 -0.26035312232456675 1102 1 1.1293686330439172 1102 2 3.7078506871657133 1102 3 2.356737730216725 1102 4 1.3995698972200614 1102 5 0.9887939085982893 1102 6 0.8781882498135545 1102 7 0.7877666134779915 1102 8 0.7085293526609462 1102 9 0.6681583458659972 1102 10 0.7691252562214572 1102 11 0.7667555946873463 1102 12 0.7124074585533009 1102 13 0.707743905488627 1102 14 0.6305559991296832 1102 15 0.7654805130442952 1102 16 0.792886497480211 1102 17 0.6561738068685727 1102 18 0.6966193262001994 1102 19 0.7857803527070502 1102 20 0.8888222374246482 1102 21 0.7122859499424834 1102 22 0.7738545764540277 1102 23 0.7409271445157088 1102 24 0.6462694326784515 1102 25 0.6003341393202319 1103 1 0.753653534265819 1103 2 2.729510732839497 1103 3 1.7211311264560862 1103 4 1.0725232272571716 1103 5 0.6738308719556088 1103 6 0.679610740932624 1103 7 0.4181595796791271 1103 8 0.30106463427238594 1103 9 0.4288054221919708 1103 10 0.2895073552946883 1103 11 0.40309087988410397 1103 12 0.3485036889688615 1103 13 0.46655265355162356 1103 14 0.5646701178933426 1103 15 0.25523405627780704 1103 16 0.1749918800785295 1103 17 0.3879791792216807 1103 18 0.41384075452197067 1103 19 0.46656380342626136 1103 20 0.505917468457028 1103 21 0.5396397256810559 1103 22 0.43003160878771496 1103 23 0.5577791345541243 1103 24 0.4150364598234333 1103 25 0.4013228867202443 1104 1 1.2390536203166285 1104 2 3.670987343851671 1104 3 2.33321702701879 1104 4 1.5638466380991716 1104 5 1.2737424107586222 1104 6 0.8564353133802172 1104 7 0.8175903065674319 1104 8 0.7316086344780715 1104 9 0.7783865371324694 1104 10 0.8583983202408376 1104 11 0.725577639830064 1104 12 0.8198231990014975 1104 13 0.6707168378546271 1104 14 0.7724798114247093 1104 15 0.6525055787223266 1104 16 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-2.0088501542355743 2315 19 -1.757199435106834 2315 20 -1.8243960392586231 2315 21 -1.6864031189218807 2315 22 -1.8872038210907434 2315 23 -1.8584077553732428 2315 24 -1.7894479450069554 2315 25 -1.7352416151029502 2316 1 -1.384225366226449 2316 2 -1.7503588195241448 2316 3 -1.0451946349298282 2316 4 -1.5286248604174943 2316 5 -1.4866219618934018 2316 6 -1.5436876019198102 2316 7 -1.5515862475556688 2316 8 -1.7107869039772297 2316 9 -1.9231173646572657 2316 10 -1.9664481569207268 2316 11 -2.2635841034672026 2316 12 -2.0949269662081704 2316 13 -2.13894672352869 2316 14 -2.5253631584750806 2316 15 -2.624972505656416 2316 16 -2.3439275707296403 2316 17 -2.4505717356530283 2316 18 -2.2514301973733595 2316 19 -2.5536934517649 2316 20 -2.563541468175174 2316 21 -2.7340499346926777 2316 22 -2.5649406489409117 2316 23 -2.6378730602933658 2316 24 -2.660677411557373 2316 25 -3.00074075412644 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 134780.0 3 4.0 4 1128.0 5 5612.0 6 6671.0 7 6145.0 8 5849.0 9 5476.0 10 5357.0 11 5208.0 12 5196.0 13 5173.0 14 5290.0 15 5570.0 16 5840.0 17 6137.0 18 7168.0 19 8710.0 20 11508.0 21 15002.0 22 18563.0 23 22188.0 24 25691.0 25 29720.0 26 33439.0 27 38157.0 28 44082.0 29 53311.0 30 66115.0 31 83956.0 32 107374.0 33 138989.0 34 184063.0 35 248146.0 36 353176.0 37 485578.0 38 984902.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.479200599253964 18.63789625005601 12.901850707764616 24.981052442925414 2 16.440237381284618 21.65480749882068 38.49119716567538 23.413757954219324 3 18.403836336018912 24.88106108843855 30.000183007212378 26.71491956833016 4 12.574709539156286 17.094419731459006 38.11289273190011 32.217977997484596 5 13.611540056176905 37.48385276880447 35.440955878223214 13.463651296795417 6 31.518795787300185 37.01049514810016 18.273238602910318 13.197470461689335 7 27.64866654003409 31.851364066344534 22.526326218559834 17.973643175061543 8 25.387920439747813 35.958805668559116 20.823337606660964 17.82993628503211 9 26.3421528084981 15.56444788301674 20.274485576191896 37.81891373229326 10 15.24945460695415 28.82029133486092 34.10730659450713 21.822947463677803 11 34.056758740329805 22.817560109981024 24.330083167312136 18.795597982377036 12 22.940143389306193 24.843260633192337 31.506837212560352 20.709758764941117 13 28.63652685125994 21.868920137545697 25.97045884956618 23.524094161628184 14 21.567943800112268 21.61224437636684 27.703384292669043 29.11642753085185 15 23.306662661543307 29.559861343638953 23.912795170124134 23.220680824693606 16 22.779728101767155 27.929645716968622 26.802668371368334 22.487957809895896 17 22.097616047082074 27.952742489289346 27.511000942171616 22.438640521456964 18 21.918963144240607 27.55571149733346 28.746110307912794 21.779215050513145 19 23.33039364016578 26.97852656688538 27.68036121939945 22.010718573549386 20 23.36935635020052 27.078317719853466 28.049929479028297 21.50239645091772 21 23.418770355608256 26.53554094723271 27.397378705659403 22.64830999149963 22 23.258087254823387 26.984052358194976 27.599813962059116 22.15804642492252 23 22.99062524749492 26.86083836053687 27.54973799852773 22.598798393440482 24 22.96611359416142 26.702910611766452 27.94692144708631 22.384054346985817 25 23.04045027346957 26.742402203154413 27.796776170189137 22.420371353186884 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 860.0 1 860.0 2 1102.5 3 1345.0 4 1345.0 5 1345.0 6 3144.0 7 4943.0 8 4943.0 9 4943.0 10 6799.0 11 8655.0 12 8655.0 13 8655.0 14 13071.0 15 17487.0 16 17487.0 17 17487.0 18 29166.5 19 40846.0 20 40846.0 21 40846.0 22 60593.0 23 80340.0 24 80340.0 25 80340.0 26 111964.0 27 143588.0 28 143588.0 29 143588.0 30 181508.0 31 219428.0 32 219428.0 33 219428.0 34 249160.5 35 278893.0 36 278893.0 37 278893.0 38 306496.0 39 334099.0 40 334099.0 41 334099.0 42 358820.5 43 383542.0 44 383542.0 45 383542.0 46 395282.5 47 407023.0 48 407023.0 49 407023.0 50 401244.5 51 395466.0 52 395466.0 53 395466.0 54 361586.5 55 327707.0 56 327707.0 57 327707.0 58 281918.0 59 236129.0 60 236129.0 61 236129.0 62 192599.0 63 149069.0 64 149069.0 65 149069.0 66 115358.5 67 81648.0 68 81648.0 69 81648.0 70 59238.0 71 36828.0 72 36828.0 73 36828.0 74 25538.5 75 14249.0 76 14249.0 77 14249.0 78 9613.0 79 4977.0 80 4977.0 81 4977.0 82 3270.5 83 1564.0 84 1564.0 85 1564.0 86 999.0 87 434.0 88 434.0 89 434.0 90 272.0 91 110.0 92 110.0 93 110.0 94 73.0 95 36.0 96 36.0 97 36.0 98 22.0 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.350018332274205 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 9.465890295379951E-5 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 4.732945147689976E-4 15 0.0 16 0.0 17 0.0 18 0.0 19 2.8397670886139853E-4 20 1.2621187060506603E-4 21 0.0 22 8.834830942354621E-4 23 5.364004500715305E-4 24 2.8397670886139853E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3169274.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.85708043057541 #Duplication Level Percentage of deduplicated Percentage of total 1 82.98336407385584 59.6294226665478 2 11.239510551282352 16.152768273675935 3 2.934224072238377 6.325343254804906 4 1.0900804280428231 3.133199879746768 5 0.55308170536535 1.987141829355888 6 0.3337881441082637 1.4391024910775998 7 0.19922652302049895 1.0021085402011516 8 0.1350717325472875 0.7764688279638087 9 0.09726771156057129 0.6290436395615383 >10 0.40625626170456414 4.862826227306787 >50 0.01644505875170501 0.8109645348394874 >100 0.009959215636465568 1.3390296153867252 >500 9.728072180622128E-4 0.5211275098050713 >1k 6.190591381351609E-4 0.7442136775487657 >5k 1.326555296003916E-4 0.6472390321777968 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8941 0.28211508376997385 No Hit TATCAACGCAGAGTACTTTTTTTTT 5789 0.1826601297331818 No Hit GGTATCAACGCAGAGTACTTTTTTT 5640 0.1779587375531431 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 3.155296765126651E-5 0.0 0.0 0.0 0.0 6 3.155296765126651E-5 0.0 0.0 0.0 0.0 7 3.155296765126651E-5 3.155296765126651E-5 0.0 0.0 0.0 8 3.155296765126651E-5 3.155296765126651E-5 0.0 0.0 0.0 9 3.155296765126651E-5 3.155296765126651E-5 0.0 3.155296765126651E-5 3.155296765126651E-5 10 3.155296765126651E-5 3.155296765126651E-5 0.0 3.155296765126651E-5 3.155296765126651E-5 11 3.155296765126651E-5 3.155296765126651E-5 0.0 6.310593530253301E-5 3.155296765126651E-5 12 3.155296765126651E-5 3.155296765126651E-5 0.0 6.310593530253301E-5 3.155296765126651E-5 13 3.155296765126651E-5 3.155296765126651E-5 0.0 6.310593530253301E-5 3.155296765126651E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1870 0.0 12.106854 1 CCAACGT 160 4.9112714E-11 11.229617 19 TAGGACC 565 0.0 11.213926 4 AATCCCG 330 0.0 11.175887 19 TACACCG 125 2.388806E-7 10.591302 5 TGTGCGA 75 0.002751588 10.086954 10 GTATCAA 3540 0.0 10.053948 1 AGTACCG 160 6.662958E-9 10.047552 5 TCCAACG 305 0.0 9.921595 18 TAGGACT 430 0.0 9.896358 4 TGGCGAG 1395 0.0 9.829357 18 GGACGTG 1480 0.0 9.775996 6 GGCGTGC 155 4.3670298E-8 9.761568 8 GTAGGAC 1500 0.0 9.64565 3 AGGACGT 1590 0.0 9.575469 5 CTAGGAC 435 0.0 9.565216 3 TTAGGAC 1140 0.0 9.539471 3 ATAGGAC 360 0.0 9.45652 3 TAGGACG 1690 0.0 9.45652 4 GACGTGG 800 0.0 9.456519 7 >>END_MODULE