FastQCFastQC Report
Thu 26 May 2016
SRR1512140_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512140_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3169274
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT117550.37090513474063774No Hit
TATCAACGCAGAGTACTTTTTTTTT78700.24832185541546742No Hit
GGTATCAACGCAGAGTACTTTTTTT75330.2376885053169906No Hit
ACGCAGAGTACTTTTTTTTTTTTTT37210.11740859263036266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21750.013.1576581
CGTATTC550.00306528612.0932083
AACTACG550.003077264612.0869127
TCCAACG2500.010.63648418
TAGGACC5400.010.5575644
AAGTCGT1553.6870915E-910.4159127
GTAGAAC8800.010.3656073
GTCTAGT2400.09.9367731
TAGAACA11400.09.8352044
AGTCGTC1554.0889063E-89.803218
GAACAGT7550.09.5597536
GTGCGCC1502.664765E-79.49656111
TGTAGGA17350.09.3648942
TCAATAC2451.8189894E-129.3078933
GTATCAA43150.09.3071761
TTAGGAC9600.09.3038533
GGCGTGC1752.6870111E-89.2255218
GTATAGA4350.09.2103611
GTAGGAC15900.09.2030083
CAATACT3100.09.1952974
Produced by FastQC (version 0.11.5)