FastQCFastQC Report
Thu 26 May 2016
SRR1512139_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512139_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3739886
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51350.13730365043212547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17900.012.1103931
GTATCAA36600.010.2500331
GATCTAG1552.1524102E-810.2177541
TATACTG5000.09.83332355
GTATTAG3150.09.7413311
TCCAACG2950.09.61537518
ACGTAAA1157.097879E-59.4680811
CTAATAC3300.09.4551183
GTCCTAC5250.09.2385531
GTGTAGC6350.09.1969841
ACCGTAT1451.8207411E-69.1293248
ACCGTGC2503.6379788E-129.0771568
GTATTAT3750.08.9745941
CTAAGAC3600.08.9298343
TCGCACT850.0077333798.8989354
TCTATAC4250.08.8989353
TAATACT3750.08.8247784
GTTCTAG3500.08.7671981
TAATACC2607.2759576E-128.7278014
TACACCG1951.7898856E-88.7278015
Produced by FastQC (version 0.11.5)