FastQCFastQC Report
Thu 26 May 2016
SRR1512139_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512139_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3739886
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64430.1722779785266182No Hit
TATCAACGCAGAGTACTTTTTTTTT42440.11347939482647333No Hit
GGTATCAACGCAGAGTACTTTTTTT38860.10390691053149749No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21550.012.2171911
CGCGCCA700.001497113510.85291110
AATCCCG3850.010.36084319
GATATAC6900.09.9538941
CCGCGCG1054.126042E-59.9485029
GCGTGCG1151.0321484E-59.9091799
GTCTTAT3650.09.6698221
GTATCAA41100.09.5159381
ACTGCGC1401.0643816E-69.4969318
GTATTAG2550.09.3521061
TAGGACC2950.09.34083754
GTCTTAG3400.09.2585841
ACCGTAT1752.687375E-89.2255918
GGTTTAG2201.546141E-109.1055511
GTATAAG3900.09.049961
ACCCGCG1054.5097445E-49.0446977
TACCGTA1054.5097445E-49.0446977
TTAGACA4250.08.9429524
GTACTAT2800.08.857781
ACTGTTC9950.08.7810828
Produced by FastQC (version 0.11.5)