FastQCFastQC Report
Thu 26 May 2016
SRR1512138_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512138_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849724
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21360.2513757408287868No Hit
GTCCTACAGTGGACATTTCTAAATT16440.19347458704238082No Hit
GTCCTAAAGTGTGTATTTCTCATTT15160.1784108722361614No Hit
CTGTAGGACGTGGAATATGGCAAGA13890.16346484270186556No Hit
GGTATCAACGCAGAGTACTTTTTTT13210.15546224421106147No Hit
TATCAACGCAGAGTACTTTTTTTTT13080.15393233567605483No Hit
CTTTAGGACGTGAAATATGGCGAGG12990.1528731682287425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3800.014.5130141
GTCTAAC550.002341116312.5339651
GTATAAG751.4823006E-411.81773951
CTAATTC658.302502E-411.6436023
GGCGAGG4450.011.48074519
TTATACT752.153584E-411.3525134
GGACGTG9500.011.3525126
GTTGTAA700.001103192211.2549911
TAATTCA855.546899E-511.1299144
TAGGACC2550.011.1299144
AGGACGT9700.011.1184415
TAGGACT1451.2987584E-911.0915364
TTAGGAC6100.011.0113163
ATTTAGA3250.010.9086831
TCTATAC700.001542993110.8119163
ACGTGAA5800.010.7653148
TAGAAAT3350.010.7312314
GACGTGA5350.010.6098257
GACGTGG4850.010.5332597
TTGAGCT909.913189E-510.5115864
Produced by FastQC (version 0.11.5)