FastQCFastQC Report
Thu 26 May 2016
SRR1512138_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512138_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849724
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34220.40271900052252263No Hit
TATCAACGCAGAGTACTTTTTTTTT28180.33163709628067467No Hit
GGTATCAACGCAGAGTACTTTTTTT26030.306334762817103No Hit
CTGTAGGACGTGGAATATGGCAAGA13880.16334715742994194No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13350.15710983801799172No Hit
CTTTAGGACGTGAAATATGGCGAGG12350.14534131082563279No Hit
GTCCTAAAGTGTGTATTTCTCATTT10620.12498175878285184No Hit
GTCCTACAGTGGACATTTCTAAATT9700.11415471376588163No Hit
GTACTTTTTTTTTTTTTTTTTTTTT9360.11015341452047958No Hit
GAGTACTTTTTTTTTTTTTTTTTTT8530.10038553695082168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8250.015.021681
CGTGCGC653.3785127E-614.61090310
GCGTGCG655.4625103E-513.1498139
TTTGGAC957.3554475E-813.0021353
AGGCGTG751.4804829E-512.6635287
TCCACCT2850.012.32955212
ATTACTC550.003062706712.0928953
CTAGTAC550.003062706712.0928953
ACTGATC550.003072173312.08791358
ACGCCAC550.00307352812.08720210
AGGCCGT658.033539E-411.689416
CCACCTT2700.011.60755213
GGCGTGC600.005882784711.0805878
GCGCCAC600.00588536211.07993513
TGCGCCA600.00588536211.07993512
CACCTTT2850.010.99662814
AAGGCGT1053.4698642E-610.8544536
ACGAAAT803.777573E-410.68422312
TAGAAAT3300.010.6532644
GCACTGT909.5264186E-510.552946