##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512138_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 849724 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.60017370346136 31.0 28.0 31.0 16.0 34.0 2 27.77995913967359 31.0 27.0 31.0 16.0 34.0 3 27.738564522127184 31.0 27.0 31.0 16.0 34.0 4 31.140274959869323 35.0 31.0 37.0 19.0 37.0 5 30.482540213057415 35.0 30.0 37.0 10.0 37.0 6 30.489520126535204 35.0 30.0 37.0 10.0 37.0 7 30.20621284087539 35.0 30.0 37.0 10.0 37.0 8 30.01050341051918 35.0 30.0 37.0 10.0 37.0 9 30.97417161337093 35.0 30.0 39.0 8.0 39.0 10 30.55777169998729 35.0 29.0 39.0 2.0 39.0 11 30.669932825246786 35.0 30.0 39.0 2.0 39.0 12 30.274802171057896 35.0 29.0 39.0 2.0 39.0 13 30.250469564234976 35.0 29.0 39.0 2.0 39.0 14 30.641961389815986 36.0 28.0 39.0 2.0 40.0 15 30.54016833701296 36.0 28.0 39.0 2.0 40.0 16 30.343405623473032 36.0 27.0 39.0 2.0 40.0 17 30.237691297409512 36.0 27.0 39.0 2.0 40.0 18 30.08007658957497 36.0 27.0 39.0 2.0 40.0 19 30.080155438707155 36.0 27.0 39.0 2.0 40.0 20 29.875636088894748 36.0 27.0 39.0 2.0 40.0 21 29.639958386487848 36.0 27.0 39.0 2.0 40.0 22 29.42165456077503 36.0 27.0 39.0 2.0 40.0 23 29.38770353667779 36.0 27.0 39.0 2.0 40.0 24 29.090768296529227 36.0 27.0 39.0 2.0 40.0 25 28.49517843440929 35.0 25.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.5791579045240489 1101 2 -0.6461149947800848 1101 3 -0.7391734984616427 1101 4 -0.8231144714527296 1101 5 -0.7722279585216683 1101 6 -0.7732290495527714 1101 7 -0.7943011532290072 1101 8 -0.9075145932841409 1101 9 -0.9307771533118476 1101 10 -1.038528211636276 1101 11 -1.0312811869406033 1101 12 -1.2326323420732486 1101 13 -1.337542876509179 1101 14 -1.3460234707795635 1101 15 -1.0374923086036993 1101 16 -1.3406142492009572 1101 17 -1.2064979193942449 1101 18 -1.3505330367696082 1101 19 -1.565026010396771 1101 20 -1.4712501857417593 1101 21 -1.4930474813599517 1101 22 -1.4332607508180786 1101 23 -1.7243923362992817 1101 24 -1.9044939228414748 1101 25 -1.807496584858967 1102 1 0.6007228480893332 1102 2 0.521729838824303 1102 3 0.5341052099847055 1102 4 0.7040936865991867 1102 5 0.06942924919683335 1102 6 0.26206941796834826 1102 7 0.7946392463479874 1102 8 0.6974939520890757 1102 9 0.7320503439151089 1102 10 1.0826906869543187 1102 11 0.48322914119655636 1102 12 0.7137438249972021 1102 13 0.8988743755780355 1102 14 0.6843106649483701 1102 15 0.319423755405154 1102 16 0.47356977754438034 1102 17 0.5633367838705787 1102 18 0.39422015578699643 1102 19 0.3373938427934604 1102 20 0.9706994521464338 1102 21 1.036898815946639 1102 22 1.0038796906454692 1102 23 1.0247925566185323 1102 24 0.9763000599746974 1102 25 0.7550552885298885 1103 1 0.05890793987846621 1103 2 0.14657016966641834 1103 3 0.44513742823688673 1103 4 0.5160827770373189 1103 5 0.16809145655469138 1103 6 0.21381187510844768 1103 7 0.14682306332332828 1103 8 0.17938245413208165 1103 9 0.29018134499946413 1103 10 0.16478539393760627 1103 11 -0.25009987503827347 1103 12 0.3509082023319259 1103 13 0.15216743292180368 1103 14 -0.025374618570722873 1103 15 0.003400717335964032 1103 16 0.24442580738822883 1103 17 0.05041850146631077 1103 18 -0.1243319401643248 1103 19 -0.20277929825122598 1103 20 0.047698713160748696 1103 21 -0.12578088327856918 1103 22 0.07957874316932134 1103 23 -0.15306174384644677 1103 24 0.1904916324905237 1103 25 0.21229950679223109 1104 1 0.35056004723466216 1104 2 0.5068423871741956 1104 3 0.36966196275493246 1104 4 0.4409068173707702 1104 5 0.4178793066021136 1104 6 0.228654052182943 1104 7 0.1765739743201813 1104 8 0.05879808500780115 1104 9 0.06678328513157794 1104 10 -0.248898693983989 1104 11 0.1452977189316762 1104 12 0.20103882014586816 1104 13 0.4105411163441559 1104 14 0.3108206592209193 1104 15 0.33285711428100484 1104 16 0.6450481139157915 1104 17 0.6025907505885932 1104 18 0.19976663052736754 1104 19 -8.291826087045706E-4 1104 20 0.2720784865346815 1104 21 0.11197681651676916 1104 22 0.09758237051753227 1104 23 0.07348112708318766 1104 24 0.09794433553051007 1104 25 0.028785638009054537 1105 1 0.556859364344092 1105 2 0.29025657457423293 1105 3 0.026922569459074452 1105 4 0.07947520265643604 1105 5 0.2179841624916321 1105 6 0.10032504360201244 1105 7 0.12918043954850944 1105 8 0.27867424887499226 1105 9 0.34876068138729366 1105 10 0.1228910898668758 1105 11 0.08002559135258736 1105 12 -0.10250039794774679 1105 13 -0.11588986790755129 1105 14 0.3341822859472252 1105 15 0.17674925871667568 1105 16 0.14056386552678646 1105 17 0.2993355724994906 1105 18 0.30275900524780397 1105 19 0.0408291331933448 1105 20 0.48193228556974077 1105 21 0.15731305219033587 1105 22 0.36209528836493377 1105 23 0.04267871407044055 1105 24 0.06059042035759532 1105 25 -0.0696435145828076 1106 1 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24 0.6162356248106029 1108 25 0.8277847662992279 1109 1 -1.0111755215093439 1109 2 -0.8873868642906189 1109 3 -1.1229724876991156 1109 4 -0.6399327702825737 1109 5 -0.7995484241286661 1109 6 -0.8332519110857746 1109 7 -0.9225071364416557 1109 8 -0.518589765579268 1109 9 -0.8877390503122378 1109 10 -0.7020982120764323 1109 11 -0.6923849694972795 1109 12 -0.7964599015579168 1109 13 -0.5223331546222632 1109 14 -0.9590273348321361 1109 15 -0.6935169361034283 1109 16 -0.7124876277860785 1109 17 -0.5282973381190921 1109 18 -0.61265638501051 1109 19 -0.7080973253083833 1109 20 -0.7141199617014031 1109 21 -0.7504758123213264 1109 22 -0.9466648917699665 1109 23 -0.8843883882165677 1109 24 -0.9475360272762643 1109 25 -1.1092528147600902 1110 1 -0.4495775654257024 1110 2 -0.6328144612558582 1110 3 -0.33430546891544566 1110 4 -0.2134453830316687 1110 5 -0.5560062908728547 1110 6 -0.43608577054741815 1110 7 -0.4246560055046267 1110 8 -0.6574371419664118 1110 9 -0.6093482523031994 1110 10 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-0.7173712218840969 2313 24 -0.705280175297716 2313 25 -0.5747873080248134 2314 1 -0.6370695572749554 2314 2 -1.0534004718948182 2314 3 -0.9120992285510603 2314 4 -0.7184197362830815 2314 5 -0.791187595405777 2314 6 -0.7693943044461768 2314 7 -0.7613207295489204 2314 8 -0.5984971524648408 2314 9 -1.2112925279737148 2314 10 -1.2614039203841543 2314 11 -0.8900701992109781 2314 12 -0.9408591360082106 2314 13 -0.7579362341058946 2314 14 -1.0802788664194267 2314 15 -0.9136696278637331 2314 16 -0.7366459343209897 2314 17 -0.6799004343299551 2314 18 -0.7776008436266366 2314 19 -0.9923348352924535 2314 20 -1.3288192966544905 2314 21 -1.467294905900438 2314 22 -1.1170275800036116 2314 23 -0.6501318510047476 2314 24 -0.7369136766260631 2314 25 -0.650525171752701 2315 1 -0.531856208590284 2315 2 -0.5995853159391977 2315 3 -0.7383500135625738 2315 4 -0.5201040220970512 2315 5 -1.1425805547247343 2315 6 -0.860041328380305 2315 7 -1.0950247627636287 2315 8 -1.3336429901593476 2315 9 -1.4378991848713483 2315 10 -0.9601121403269346 2315 11 -0.808607809984828 2315 12 -1.126959198809132 2315 13 -1.1049723795249164 2315 14 -1.0559217036254829 2315 15 -1.0794858653907724 2315 16 -1.0148452923560178 2315 17 -1.4636602905856257 2315 18 -1.5392320975516967 2315 19 -1.083775593090234 2315 20 -1.4095402861178918 2315 21 -1.2916442185792434 2315 22 -1.476314266208341 2315 23 -0.9172102125984942 2315 24 -0.7999544623353643 2315 25 -0.8839527064676567 2316 1 -1.1169349370513686 2316 2 -1.4290564983515566 2316 3 -1.3033381300212774 2316 4 -1.358005091615766 2316 5 -1.4204697407021563 2316 6 -1.377903776389811 2316 7 -1.317878298117165 2316 8 -1.29026806443148 2316 9 -1.545816594259346 2316 10 -1.7766228355141003 2316 11 -1.625192777305088 2316 12 -1.4023602683278469 2316 13 -1.3421233004993667 2316 14 -1.6190158806902488 2316 15 -1.7865937085280272 2316 16 -1.8044323392960067 2316 17 -1.609939256717535 2316 18 -1.8730481997501514 2316 19 -1.4207111248787072 2316 20 -1.6674502682925798 2316 21 -1.6298052405637975 2316 22 -1.6866158110687088 2316 23 -1.7711911989324207 2316 24 -1.8047450149200372 2316 25 -1.7469206209061596 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 54929.0 3 4.0 4 785.0 5 5928.0 6 7054.0 7 6056.0 8 4878.0 9 4220.0 10 3916.0 11 3827.0 12 3614.0 13 3565.0 14 3497.0 15 3335.0 16 3538.0 17 3666.0 18 4008.0 19 4581.0 20 5434.0 21 6948.0 22 8736.0 23 10628.0 24 12275.0 25 14202.0 26 16301.0 27 18228.0 28 20993.0 29 25179.0 30 30256.0 31 36881.0 32 45212.0 33 55205.0 34 66369.0 35 79996.0 36 96037.0 37 106011.0 38 73432.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.62397159803567 23.99805362453378 15.34306048645214 19.034914290978406 2 18.958155824714847 25.339522009499554 36.98600957487373 18.716312590911873 3 22.029388366104758 28.542915111259653 30.307841134297725 19.11985538833786 4 18.21497333251738 21.341753322255226 35.798329810620864 24.64494353460653 5 13.849146401628659 40.7532436031636 34.46208407758508 10.935525917622664 6 31.16023555884028 40.20517250307159 17.13262188663613 11.501970051452002 7 27.780785290282488 33.97609106015601 22.434108016249983 15.809015633311521 8 26.086593220877557 38.88044797145335 19.8760263952915 15.15693241237759 9 27.874580452005592 15.988838728810768 20.399682720506895 35.73689809867675 10 16.43310063032232 31.298280382806652 33.29975380241114 18.968865184459897 11 35.38654904416022 23.14292640904576 24.49901379742128 16.971510749372737 12 24.033921602779255 26.523435845050862 31.333821334927574 18.108821217242305 13 30.507670726023978 23.077140342040476 25.645386031228963 20.769802900706583 14 22.208269979428614 23.66391910785149 27.51999472769982 26.607816185020077 15 24.10159063413532 32.493492004462624 23.205652658981034 20.199264702421022 16 23.011825016122884 29.504874523963075 27.323931064675115 20.159369395238926 17 22.55085180599818 30.138609713271602 27.332286718981692 19.97825176174852 18 22.339489057623414 28.4538273604135 29.813209936402878 19.393473645560206 19 24.507369451727858 27.792318446931002 28.004740362753083 19.695571738588058 20 24.58751312190782 28.339084220287997 28.11230470129124 18.961097956512937 21 24.071463204522882 27.540236594470674 27.588487555959347 20.799812645047098 22 24.524433816156776 28.553624471004703 27.696051894497508 19.225889818341013 23 23.411366514303467 28.64471287147356 28.079588195696488 19.864332418526487 24 24.079827887741004 28.00955185376107 28.26741424398419 19.643206014513733 25 23.734316874166637 28.462350494692952 28.266050306042352 19.53728232509806 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 400.0 1 400.0 2 425.5 3 451.0 4 451.0 5 451.0 6 925.0 7 1399.0 8 1399.0 9 1399.0 10 1966.0 11 2533.0 12 2533.0 13 2533.0 14 3819.0 15 5105.0 16 5105.0 17 5105.0 18 9085.0 19 13065.0 20 13065.0 21 13065.0 22 20322.5 23 27580.0 24 27580.0 25 27580.0 26 39275.0 27 50970.0 28 50970.0 29 50970.0 30 63331.5 31 75693.0 32 75693.0 33 75693.0 34 83188.5 35 90684.0 36 90684.0 37 90684.0 38 94698.5 39 98713.0 40 98713.0 41 98713.0 42 101757.0 43 104801.0 44 104801.0 45 104801.0 46 103741.5 47 102682.0 48 102682.0 49 102682.0 50 98042.5 51 93403.0 52 93403.0 53 93403.0 54 82658.5 55 71914.0 56 71914.0 57 71914.0 58 60833.5 59 49753.0 60 49753.0 61 49753.0 62 40050.0 63 30347.0 64 30347.0 65 30347.0 66 23542.0 67 16737.0 68 16737.0 69 16737.0 70 12386.0 71 8035.0 72 8035.0 73 8035.0 74 5795.5 75 3556.0 76 3556.0 77 3556.0 78 2440.5 79 1325.0 80 1325.0 81 1325.0 82 866.0 83 407.0 84 407.0 85 407.0 86 273.5 87 140.0 88 140.0 89 140.0 90 82.5 91 25.0 92 25.0 93 25.0 94 15.0 95 5.0 96 5.0 97 5.0 98 3.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3556448917530869 2 0.0 3 0.0 4 0.0 5 0.05142846383060852 6 0.0 7 0.0 8 0.003177502341936911 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.005413522508485109 25 5.884263596179465E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 849724.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.48988998933532 #Duplication Level Percentage of deduplicated Percentage of total 1 89.22205829758254 69.13807482101734 2 7.240414331697435 11.221178200808822 3 1.7427376977014895 4.0513365742546785 4 0.6952062627241519 2.1548582727356593 5 0.3457752109440583 1.3397041528547147 6 0.19342265365345743 0.8992980091831035 7 0.11373806860623817 0.6169485296727809 8 0.07257740436722164 0.4499212064102009 9 0.06196351692424444 0.4321391498830818 >10 0.2741842202611187 3.5850086075194785 >50 0.01956956186506781 1.0634721849334023 >100 0.015622609154644578 2.4410515980819043 >500 0.0016684338723200528 0.977642951418756 >1k 0.0010617306460218518 1.6293657412260416 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3422 0.40271900052252263 No Hit TATCAACGCAGAGTACTTTTTTTTT 2818 0.33163709628067467 No Hit GGTATCAACGCAGAGTACTTTTTTT 2603 0.306334762817103 No Hit CTGTAGGACGTGGAATATGGCAAGA 1388 0.16334715742994194 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1335 0.15710983801799172 No Hit CTTTAGGACGTGAAATATGGCGAGG 1235 0.14534131082563279 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1062 0.12498175878285184 No Hit GTCCTACAGTGGACATTTCTAAATT 970 0.11415471376588163 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 936 0.11015341452047958 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 853 0.10038553695082168 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.1768527192358931E-4 0.0 0.0 0.0 6 0.0 1.1768527192358931E-4 0.0 0.0 0.0 7 0.0 2.3537054384717863E-4 0.0 0.0 0.0 8 0.0 2.3537054384717863E-4 0.0 0.0 0.0 9 0.0 2.3537054384717863E-4 0.0 0.0 0.0 10 0.0 2.3537054384717863E-4 0.0 0.0 0.0 11 0.0 2.3537054384717863E-4 0.0 0.0 0.0 12 0.0 2.3537054384717863E-4 0.0 0.0 0.0 13 0.0 3.530558157707679E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 825 0.0 15.02168 1 CGTGCGC 65 3.3785127E-6 14.610903 10 GCGTGCG 65 5.4625103E-5 13.149813 9 TTTGGAC 95 7.3554475E-8 13.002135 3 AGGCGTG 75 1.4804829E-5 12.663528 7 TCCACCT 285 0.0 12.329552 12 ATTACTC 55 0.0030627067 12.092895 3 CTAGTAC 55 0.0030627067 12.092895 3 ACTGATC 55 0.0030721733 12.0879135 8 ACGCCAC 55 0.003073528 12.087202 10 AGGCCGT 65 8.033539E-4 11.68941 6 CCACCTT 270 0.0 11.607552 13 GGCGTGC 60 0.0058827847 11.080587 8 GCGCCAC 60 0.005885362 11.079935 13 TGCGCCA 60 0.005885362 11.079935 12 CACCTTT 285 0.0 10.996628 14 AAGGCGT 105 3.4698642E-6 10.854453 6 ACGAAAT 80 3.777573E-4 10.684223 12 TAGAAAT 330 0.0 10.653264 4 GCACTGT 90 9.5264186E-5 10.55294 6 >>END_MODULE