FastQCFastQC Report
Thu 26 May 2016
SRR1512137_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512137_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2859731
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67900.23743491957810017No Hit
GGTATCAACGCAGAGTACTTTTTTT43830.15326616384548059No Hit
TATCAACGCAGAGTACTTTTTTTTT43540.15225208245111166No Hit
GTCCTACAGTGGACATTTCTAAATT40630.1420763001834788No Hit
GTCCTAAAGTGTGTATTTCTCATTT39240.13721570315529677No Hit
CTTTAGGACGTGAAATATGGCGAGG37640.1316207713242959No Hit
CTGTAGGACGTGGAATATGGCAAGA36880.12896317870457047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18450.012.6619751
TAGGACC7300.011.2684174
AGAACCG803.94151E-410.6369975
CTAGGAC3850.010.5602513
GGCGAGG13550.010.46709319
GCCGGTT2001.8189894E-1210.400619511
TCCAACG2300.010.277472518
TTAGGAC17800.010.0925313
TAGGACA9700.010.0399624
AATCCCG4000.09.92803719
CGTTAGG1104.3578482E-59.8988731
CCGGTTT2201.8189894E-129.88488712
GTATTAA3650.09.7632731
GACGTGA14750.09.7435697
TGTAGAA10100.09.7028572
GATATAC8600.09.6686681
CGGTTTC2253.6379788E-129.66539113
TGGCGAG27850.09.60805218
AGGACGT27150.09.5769985
GGACGTG27100.09.5597786
Produced by FastQC (version 0.11.5)