FastQCFastQC Report
Thu 26 May 2016
SRR1512137_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512137_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2859731
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT87280.30520353138109846No Hit
TATCAACGCAGAGTACTTTTTTTTT60330.21096389835267723No Hit
GGTATCAACGCAGAGTACTTTTTTT58910.20599839635266395No Hit
GTCCTACAGTGGACATTTCTAAATT45550.15928071556380652No Hit
GTCCTAAAGTGTGTATTTCTCATTT44480.15553910490182468No Hit
CTTTAGGACGTGAAATATGGCGAGG42780.14959448983138623No Hit
CTGTAGGACGTGGAATATGGCAAGA41880.14644734067644824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20850.011.5740541
TCCAACG3000.011.39632818
TAGGACC8150.011.19284254
CCCGCAC600.0058673211.0859673
GGCGAGG12300.010.34644119
GTCCTAC24450.010.1819911
GTCCTAA14600.09.9956171
CCAACGA2300.09.91002419
GTTAGAC1951.6370905E-109.7459053
TTAGGAC17950.09.5816633
GTATTAA3600.09.5383011
GTACTAG1701.560511E-89.53830051
TCCTACA27700.09.4679532
AGGACGT26500.09.3229695
TGGCGAG25400.09.30999318
GTATCAA39050.09.2574031
GGACGTG26400.09.2451286
GACGTGA15350.09.2185287
ATAGGAC3450.09.0891153
ATTTCTA19950.09.0442315
Produced by FastQC (version 0.11.5)