FastQCFastQC Report
Thu 26 May 2016
SRR1512136_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512136_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2247335
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74510.33154825604549387No Hit
TATCAACGCAGAGTACTTTTTTTTT49030.2181695207879555No Hit
GGTATCAACGCAGAGTACTTTTTTT47570.21167293705655812No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG550.003181571412.0329729
GGTATCA16000.010.8959241
TAGGACC3300.010.6004764
TGTACCG1002.5302696E-510.3999265
GTATTAG2101.8189894E-1210.3770691
TCTATAC2200.010.3139763
GTATCAA32050.010.1062751
CTAACCT1502.63517E-810.0847784
CCTTATA1957.894414E-109.6514012
AGGACCG1502.840461E-79.4544795
CTAGTAC1107.207146E-59.4544783
GGACCGT900.00116393179.4544786
GTCTAGC1501.5332207E-69.2450261
TTAGACC1554.5682282E-79.1494954
CCGTGTG1554.5682282E-79.1494959
TTAGGAC5200.09.0908453
GTCTTAC2551.6370905E-118.9342691
AATCCCG1701.8139508E-78.89833319
ACCGAGA850.00773567158.8983338
TACGCTG850.00773567158.8983335
Produced by FastQC (version 0.11.5)