FastQCFastQC Report
Thu 26 May 2016
SRR1512136_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512136_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2247335
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT95700.42583771444844665No Hit
TATCAACGCAGAGTACTTTTTTTTT66330.2951495882901303No Hit
GGTATCAACGCAGAGTACTTTTTTT64610.287496078688758No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30250.13460387525669293No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25960.11551459840210739No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20450.012.1765161
TAGGACC4000.011.4025924
ACGCTAT600.00589340611.07919312
TGGCGAG8850.010.62314818
GGACGTG9600.010.4858986
TCCAACG2100.010.40087518
AGGACGT9650.010.3391375
GTCCTAA5600.010.22207451
TAGGACG10150.010.0170554
GACGTGG4950.09.9762927
TCGAACT1054.1233143E-59.94866419
GGCGAGG4700.09.90055619
TATGGCG9350.09.851933516
TAAGACT3100.09.8086814
GGCGAGA5000.09.68638119
GAACAGT4550.09.6010226
GTAGGAC10500.09.5926563
TGTAGGA11550.09.5432942
CTAAGAC2106.730261E-119.502163
CGTGCGC900.0011177339.49645110
Produced by FastQC (version 0.11.5)