FastQCFastQC Report
Thu 26 May 2016
SRR1512135_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512135_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3002134
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62080.20678623938838173No Hit
GGTATCAACGCAGAGTACTTTTTTT37990.1265433188525229No Hit
TATCAACGCAGAGTACTTTTTTTTT37250.12407840556084439No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACC707.631112E-613.5074978
GGTATCA18000.012.3738531
GTCCTAA7000.010.3233291
GTTCCGT750.002754966510.0854296
AAGACCG1151.0866444E-59.8661815
GTATCAA34300.09.8125021
AATCCCG1554.3768523E-89.76009319
GTCTTAG2850.09.7254151
CTAACAC3050.09.6100913
GTACCGT1607.1449904E-89.455096
GTATTAG2950.09.395741
GTATAAC2352.910383E-119.2672271
GTCCTAC11200.09.1920051
TACTATA3150.09.1134412
GTAGGAC10800.09.1049013
GTCTACG1000.00200072688.9091741
ACGATAC850.00773283338.8989083
TAGGACG9450.08.804744
TAGGACC5800.08.8030154
TCCTACA12600.08.7206212
Produced by FastQC (version 0.11.5)