FastQCFastQC Report
Thu 26 May 2016
SRR1512135_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512135_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3002134
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT78660.2620136209776113No Hit
TATCAACGCAGAGTACTTTTTTTTT52310.1742427220104099No Hit
GGTATCAACGCAGAGTACTTTTTTT49110.16358363750585417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18950.012.6367581
AACCGTG1002.4097943E-510.4463087
ACCGCTC856.618218E-410.055278
GTATCAA37850.09.7043391
GTAGGAC10600.09.6809853
GTACTAG1606.2198524E-89.5405011
TAGGACG9600.09.4027324
GTGGTAT6700.09.255711
TAGGACC4850.09.2078414
GGACGTG9650.08.9553846
GGTAGCG850.0074597138.9380187
TTAGGAC7450.08.9277793
TAGACCA6400.08.9078514
ACCGTGC1606.780192E-78.9031048
GACGTGA6150.08.8017687
GTATTAA3700.08.7669471
AGGACGT10300.08.7637115
TCGAACT2502.5465852E-118.73749419
TAATACC2401.0186341E-108.7098994
GTCTAGA3400.08.6986911
Produced by FastQC (version 0.11.5)