##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512135_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3002134 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.06614827985693 31.0 31.0 34.0 28.0 34.0 2 31.24769280784935 31.0 31.0 34.0 28.0 34.0 3 31.349269552924685 33.0 31.0 34.0 28.0 34.0 4 34.946933414697675 37.0 35.0 37.0 32.0 37.0 5 34.58364183610725 37.0 35.0 37.0 31.0 37.0 6 34.538586552099275 37.0 35.0 37.0 31.0 37.0 7 34.37863199977083 37.0 35.0 37.0 30.0 37.0 8 34.2895956676151 37.0 35.0 37.0 30.0 37.0 9 35.81212097794435 39.0 35.0 39.0 30.0 39.0 10 35.63650922976789 39.0 35.0 39.0 30.0 39.0 11 35.63197811956428 39.0 35.0 39.0 30.0 39.0 12 35.461257558789846 39.0 35.0 39.0 30.0 39.0 13 35.42762614859963 39.0 35.0 39.0 30.0 39.0 14 36.48880896055939 40.0 36.0 41.0 30.0 41.0 15 36.419629503546474 40.0 36.0 41.0 30.0 41.0 16 36.32594381196842 40.0 36.0 41.0 29.0 41.0 17 36.26011896870693 39.0 36.0 41.0 29.0 41.0 18 36.184865499008374 39.0 36.0 41.0 29.0 41.0 19 36.195197482857196 39.0 36.0 41.0 29.0 41.0 20 36.09030975965763 39.0 36.0 41.0 28.0 41.0 21 35.97462371766217 39.0 36.0 41.0 28.0 41.0 22 35.86290818464466 39.0 36.0 41.0 27.0 41.0 23 35.933666518549806 39.0 36.0 41.0 29.0 41.0 24 35.82866587567377 39.0 36.0 41.0 28.0 41.0 25 35.40583065246255 39.0 35.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1303995548499408 1101 2 0.05181657946519991 1101 3 0.04930191049813004 1101 4 -0.017069726671643082 1101 5 0.12567145680051084 1101 6 0.025183062627000652 1101 7 0.1193241591632912 1101 8 -0.04145453149585876 1101 9 -0.12593698498588424 1101 10 0.019303502751981227 1101 11 -0.15954061251667184 1101 12 -0.20919726873384548 1101 13 0.018249291260147515 1101 14 -0.05925737942430942 1101 15 0.04257184471512687 1101 16 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0.41124800082371493 1103 3 0.4122321060560772 1103 4 0.33604929282493856 1103 5 0.4261916543086812 1103 6 0.4855289833256933 1103 7 0.4649218602030274 1103 8 0.5171655050762851 1103 9 0.614685989396726 1103 10 0.4736951330018897 1103 11 0.456853794044882 1103 12 0.6066117290509254 1103 13 0.5832618673695293 1103 14 0.6208754753831087 1103 15 0.5875194150998624 1103 16 0.7257261306918679 1103 17 0.6816860488042735 1103 18 0.7617904020031929 1103 19 0.6354787214786981 1103 20 0.7554943147013233 1103 21 0.7691510308977669 1103 22 0.7401904660812235 1103 23 0.626326091020637 1103 24 0.7547707582905829 1103 25 0.7073681915374479 1104 1 0.04050476670767722 1104 2 -0.027510659817917116 1104 3 -0.0047193503909070955 1104 4 -0.07354176182420957 1104 5 -0.04212840993257316 1104 6 -0.021451372710153294 1104 7 0.0356520877828288 1104 8 -0.06563377909126444 1104 9 0.0540980640691302 1104 10 -0.10537653804752978 1104 11 0.252754087068503 1104 12 0.21737869879905247 1104 13 0.16864431193664586 1104 14 0.13201694412389742 1104 15 0.24317413354205542 1104 16 0.4081002814578909 1104 17 0.3527162507331454 1104 18 0.40769465947087014 1104 19 0.4205531477304305 1104 20 0.32478905678978265 1104 21 0.3599916345842047 1104 22 0.31565788784502047 1104 23 0.2014357346545168 1104 24 0.07906124651330515 1104 25 0.27665453291716346 1105 1 0.05818651715705769 1105 2 0.09066643115686546 1105 3 0.09438934273716981 1105 4 0.12296227652018388 1105 5 0.13059502477440077 1105 6 0.16843448505002812 1105 7 0.17413351592318804 1105 8 0.16151955822768116 1105 9 -0.05608916992528634 1105 10 0.15091203099423467 1105 11 0.12543104513406433 1105 12 -0.025888093424413228 1105 13 0.04689547277144612 1105 14 0.2777909132789773 1105 15 0.29886725596392694 1105 16 0.21524085017628636 1105 17 0.33407203125295126 1105 18 0.10512174320967205 1105 19 0.10681219728478197 1105 20 0.11271051958301825 1105 21 0.03220588890691545 1105 22 0.15102478217071535 1105 23 -0.008617059749390421 1105 24 0.11252276488555424 1105 25 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-0.7919073892565862 2314 18 -0.945221636957065 2314 19 -1.0459120103368207 2314 20 -1.0753940879686255 2314 21 -0.9352126384643853 2314 22 -0.9507330250155945 2314 23 -0.8339892006771166 2314 24 -0.7376835158427966 2314 25 -0.9116468117899004 2315 1 -0.6323668866900789 2315 2 -0.6637296822628151 2315 3 -0.663985438640772 2315 4 -0.7346085266106428 2315 5 -0.8055423713568146 2315 6 -0.8029870714529252 2315 7 -0.8117406729299219 2315 8 -0.8105023660519493 2315 9 -1.0636878346354237 2315 10 -0.9219110404211222 2315 11 -0.8391517513880302 2315 12 -0.9022234263258042 2315 13 -0.8910420702332331 2315 14 -1.114397495653634 2315 15 -1.2457037149293768 2315 16 -1.2410089142962946 2315 17 -1.1907365387507767 2315 18 -1.1040785462523388 2315 19 -1.2203377400960065 2315 20 -1.3255669480796612 2315 21 -1.121530632946488 2315 22 -1.1195958950043519 2315 23 -1.2949100426676452 2315 24 -1.2082456943911097 2315 25 -1.2794425473394782 2316 1 -0.9268513500031048 2316 2 -1.030535706828406 2316 3 -1.1000866820369133 2316 4 -0.926988882078966 2316 5 -1.1036653632963862 2316 6 -1.2219219603338445 2316 7 -1.2031548855140244 2316 8 -1.2649283618193294 2316 9 -1.4242672705594046 2316 10 -1.4110068239426 2316 11 -1.4724438879899395 2316 12 -1.5547727663240636 2316 13 -1.496512801871809 2316 14 -1.6387920670368175 2316 15 -1.6671525225194017 2316 16 -1.7764058633255075 2316 17 -1.7372125688505449 2316 18 -1.7382988103042791 2316 19 -1.6484744778016847 2316 20 -1.7284328698530587 2316 21 -1.7505249757887 2316 22 -1.782040937561142 2316 23 -1.7325186124418224 2316 24 -1.684665556446646 2316 25 -1.7978375978388357 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37132.0 3 1.0 4 675.0 5 6090.0 6 7572.0 7 6237.0 8 5270.0 9 4772.0 10 4475.0 11 4374.0 12 4234.0 13 4149.0 14 4080.0 15 4212.0 16 4342.0 17 4525.0 18 4851.0 19 5543.0 20 6732.0 21 8656.0 22 11256.0 23 14151.0 24 17756.0 25 21111.0 26 24987.0 27 28516.0 28 33993.0 29 41653.0 30 53807.0 31 69535.0 32 90560.0 33 118073.0 34 155968.0 35 206134.0 36 298402.0 37 517823.0 38 1170487.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.43148481438691 19.142428005060367 13.52630576933179 24.899781411220935 2 15.607797653269309 20.77245719211734 39.12496910530975 24.494776049303596 3 18.451541470167555 24.87996871558698 30.132165985928676 26.53632382831679 4 13.299106568860683 17.42930195654158 37.18161814229478 32.089973332302954 5 13.949280434072564 37.40579652182028 35.02086374574226 13.624059298364896 6 32.05286639437147 36.70658937942144 17.871720582758797 13.368823643448295 7 28.244109023781082 31.675967828218194 22.107840622703716 17.972082525297004 8 25.959025739574308 35.47854768988062 20.383966721196416 18.17845984934866 9 26.65384023497952 15.448910674873273 19.88655403123245 38.010695058914756 10 15.725613846683725 28.45502565841498 33.46599452256295 22.35336597233834 11 34.43210729434462 22.641327802156734 23.919785059560965 19.00677984393768 12 23.400554405632793 24.618221571721982 30.79589385417173 21.18533016847349 13 28.730596302496824 21.513563351935655 25.856673952595056 23.899166392972464 14 22.013740892311937 21.07307668478489 27.248950246724498 29.66423217617868 15 23.753303483455433 28.97369004847885 23.60747388357748 23.665532584488233 16 23.39549134049313 27.63997209984631 25.94920812995023 23.015328429710333 17 22.64276011663703 27.61975314892673 26.980108149736154 22.757378584700085 18 22.602222285880643 27.13143384006177 27.953848828866402 22.312495045191188 19 23.79614600172078 26.811239623613776 27.208590969941664 22.18402340472378 20 23.941236467126384 26.59088501712449 27.28978786423258 22.178090651516555 21 23.95609256615461 26.13660815939595 26.958390265058124 22.94890900939132 22 23.952162028743555 26.503413904908975 26.940236511761302 22.604187554586172 23 23.5023486626513 26.390161132048068 27.012385190001513 23.095105015299115 24 23.435934368737474 26.373325107662133 27.27396281925553 22.91677770434486 25 23.5618283644111 26.373433467917216 27.152090225052987 22.9126479426187 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 956.0 1 956.0 2 914.5 3 873.0 4 873.0 5 873.0 6 1975.0 7 3077.0 8 3077.0 9 3077.0 10 4425.5 11 5774.0 12 5774.0 13 5774.0 14 9175.5 15 12577.0 16 12577.0 17 12577.0 18 22416.0 19 32255.0 20 32255.0 21 32255.0 22 49731.5 23 67208.0 24 67208.0 25 67208.0 26 94566.5 27 121925.0 28 121925.0 29 121925.0 30 154798.0 31 187671.0 32 187671.0 33 187671.0 34 218866.5 35 250062.0 36 250062.0 37 250062.0 38 281256.5 39 312451.0 40 312451.0 41 312451.0 42 338918.5 43 365386.0 44 365386.0 45 365386.0 46 378388.5 47 391391.0 48 391391.0 49 391391.0 50 389451.5 51 387512.0 52 387512.0 53 387512.0 54 356449.5 55 325387.0 56 325387.0 57 325387.0 58 282572.5 59 239758.0 60 239758.0 61 239758.0 62 196694.5 63 153631.0 64 153631.0 65 153631.0 66 118802.5 67 83974.0 68 83974.0 69 83974.0 70 61200.5 71 38427.0 72 38427.0 73 38427.0 74 26540.0 75 14653.0 76 14653.0 77 14653.0 78 9959.5 79 5266.0 80 5266.0 81 5266.0 82 3362.0 83 1458.0 84 1458.0 85 1458.0 86 922.0 87 386.0 88 386.0 89 386.0 90 228.5 91 71.0 92 71.0 93 71.0 94 37.0 95 3.0 96 3.0 97 3.0 98 2.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4469154274925769 2 0.0 3 0.0 4 0.0 5 0.05709272137752678 6 0.0 7 0.0 8 0.003430892824903885 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.6654819538368374E-4 20 0.0 21 0.0 22 0.0 23 0.0 24 0.004996445861510512 25 0.0013656952021462068 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3002134.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.13057165437283 #Duplication Level Percentage of deduplicated Percentage of total 1 76.71889661474759 49.20026696596492 2 14.127251546809719 18.119774352040604 3 4.4890296574410415 8.636521143153825 4 1.8502373528379987 4.746271165350976 5 0.9211487451598354 2.9536897802904223 6 0.5254269761653559 2.0217559406467687 7 0.3210063184627473 1.441042309537715 8 0.21266062267829683 1.091043784058725 9 0.14568493879280178 0.8408572565593226 >10 0.6502158631918746 6.930687081198815 >50 0.025571043857094328 1.1121129122054405 >100 0.011311698842952956 1.319187611953027 >500 7.793105067195554E-4 0.37259987802040057 >1k 6.754024385282585E-4 0.777818487596669 >5k 1.0390806746588594E-4 0.4363713314222992 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7866 0.2620136209776113 No Hit TATCAACGCAGAGTACTTTTTTTTT 5231 0.1742427220104099 No Hit GGTATCAACGCAGAGTACTTTTTTT 4911 0.16358363750585417 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.330963907673675E-5 0.0 3 0.0 0.0 0.0 3.330963907673675E-5 0.0 4 0.0 0.0 0.0 3.330963907673675E-5 0.0 5 0.0 0.0 0.0 3.330963907673675E-5 0.0 6 0.0 0.0 0.0 3.330963907673675E-5 0.0 7 0.0 0.0 0.0 3.330963907673675E-5 0.0 8 0.0 0.0 0.0 3.330963907673675E-5 0.0 9 0.0 0.0 0.0 3.330963907673675E-5 0.0 10 0.0 0.0 0.0 3.330963907673675E-5 0.0 11 0.0 0.0 0.0 3.330963907673675E-5 0.0 12 0.0 0.0 0.0 3.330963907673675E-5 0.0 13 0.0 3.330963907673675E-5 0.0 3.330963907673675E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1895 0.0 12.636758 1 AACCGTG 100 2.4097943E-5 10.446308 7 ACCGCTC 85 6.618218E-4 10.05527 8 GTATCAA 3785 0.0 9.704339 1 GTAGGAC 1060 0.0 9.680985 3 GTACTAG 160 6.2198524E-8 9.540501 1 TAGGACG 960 0.0 9.402732 4 GTGGTAT 670 0.0 9.25571 1 TAGGACC 485 0.0 9.207841 4 GGACGTG 965 0.0 8.955384 6 GGTAGCG 85 0.007459713 8.938018 7 TTAGGAC 745 0.0 8.927779 3 TAGACCA 640 0.0 8.907851 4 ACCGTGC 160 6.780192E-7 8.903104 8 GACGTGA 615 0.0 8.801768 7 GTATTAA 370 0.0 8.766947 1 AGGACGT 1030 0.0 8.763711 5 TCGAACT 250 2.5465852E-11 8.737494 19 TAATACC 240 1.0186341E-10 8.709899 4 GTCTAGA 340 0.0 8.698691 1 >>END_MODULE