FastQCFastQC Report
Thu 26 May 2016
SRR1512134_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512134_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2963864
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67860.22895787391054379No Hit
GTCCTACAGTGGACATTTCTAAATT42480.1433264144373696No Hit
GGTATCAACGCAGAGTACTTTTTTT41280.13927764566795237No Hit
CTTTAGGACGTGAAATATGGCGAGG40790.13762439842044033No Hit
GTCCTAAAGTGTGTATTTCTCATTT40790.13762439842044033No Hit
TATCAACGCAGAGTACTTTTTTTTT40600.13698334336528262No Hit
CTGTAGGACGTGGAATATGGCAAGA40470.13654472674859575No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCGC400.00544420514.184615
GTCGTAG500.001110013813.8416421
GGTATCA15400.011.9413071
TAGGACC9750.011.6388434
TCCAACG2800.011.48277118
TTAGGAC15700.010.9021473
CGGTAGG1302.220786E-710.6474171
GACGTGA14250.010.5515297
AATCCCG3950.010.53371119
AGGACGT26600.010.5230885
GGACGTG26350.010.371716
CCAACGT2100.010.35700919
GTATCAA31950.010.3355881
GTCCTAA13900.010.1714031
GGCGAGG12800.010.12130319
GTCCTAC24150.010.07111551
GATATAC7300.09.88688851
GTACTAG1501.4826219E-79.88688851
GTACCGT1151.0846266E-59.8677136
TAGGACT3750.09.8348224
Produced by FastQC (version 0.11.5)