##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512134_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2963864 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.94679074343492 31.0 28.0 33.0 20.0 34.0 2 26.408333175881214 31.0 25.0 34.0 10.0 34.0 3 28.082318217030203 31.0 27.0 34.0 16.0 34.0 4 32.36330411921735 35.0 32.0 37.0 22.0 37.0 5 32.76098296008184 35.0 33.0 37.0 25.0 37.0 6 33.016565874817466 36.0 35.0 37.0 26.0 37.0 7 33.063317682592725 37.0 35.0 37.0 26.0 37.0 8 33.102835015371824 37.0 35.0 37.0 26.0 37.0 9 34.64417800546854 39.0 35.0 39.0 27.0 39.0 10 34.49206272622496 39.0 35.0 39.0 27.0 39.0 11 34.51042996574741 39.0 35.0 39.0 27.0 39.0 12 34.40152449640065 38.0 35.0 39.0 27.0 39.0 13 34.39425661906214 39.0 35.0 39.0 27.0 39.0 14 35.44478862727844 39.0 36.0 41.0 27.0 41.0 15 35.37376343853834 39.0 36.0 41.0 26.0 41.0 16 35.337135239673614 39.0 36.0 41.0 26.0 41.0 17 35.27010551091413 39.0 35.0 41.0 26.0 41.0 18 35.21717123322797 39.0 35.0 41.0 25.0 41.0 19 35.23713436244038 39.0 35.0 41.0 25.0 41.0 20 35.165664483930435 39.0 35.0 41.0 25.0 41.0 21 35.08961848451886 39.0 35.0 41.0 25.0 41.0 22 34.97626071911532 39.0 35.0 41.0 25.0 41.0 23 34.8596163656632 39.0 35.0 41.0 24.0 41.0 24 34.73911319817643 39.0 35.0 41.0 23.0 41.0 25 34.60475143258935 39.0 34.0 41.0 22.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0290519988208828 1101 2 3.3183959413011763 1101 3 1.798190256658053 1101 4 1.0147574091448632 1101 5 0.8748266902366737 1101 6 0.35780352462244736 1101 7 0.21820172734090448 1101 8 0.3299335022556633 1101 9 0.26249367345945984 1101 10 0.02501355193930266 1101 11 0.2535532107434477 1101 12 0.13733079519001734 1101 13 0.04822114103261299 1101 14 0.18901576716992707 1101 15 0.22063264121281634 1101 16 0.132799376527295 1101 17 0.019655932044869928 1101 18 -0.03699276248492822 1101 19 0.07465594726910041 1101 20 -0.03912458890730619 1101 21 0.14466204364647695 1101 22 -0.0024647280132512606 1101 23 -0.10454031198718639 1101 24 -0.07659748725627225 1101 25 -0.0321677247922878 1102 1 1.2874735801077861 1102 2 3.815609112080555 1102 3 2.4320831378195322 1102 4 1.4160078479029181 1102 5 1.0708100662941007 1102 6 0.9561805679916873 1102 7 0.8331477781714938 1102 8 0.7781149477198426 1102 9 0.7149820315531059 1102 10 0.8178981251773436 1102 11 0.8282955506206093 1102 12 0.767866620688352 1102 13 0.7672179912268859 1102 14 0.7391654508882581 1102 15 0.7215174434435312 1102 16 0.8224413326104383 1102 17 0.6393571660030943 1102 18 0.5726412366812141 1102 19 0.6259746501297201 1102 20 0.8163723627723485 1102 21 0.7653600896335249 1102 22 0.7821763404341624 1102 23 0.7490341421457529 1102 24 0.7639297047558173 1102 25 0.6770277765426371 1103 1 0.9156295567636512 1103 2 2.8488623981925976 1103 3 1.7876341263980216 1103 4 1.1146016256156344 1103 5 0.8628276785596256 1103 6 0.7533405338707411 1103 7 0.5734935483843557 1103 8 0.41173872202609374 1103 9 0.6026779384468313 1103 10 0.39621386782881274 1103 11 0.39675337943592126 1103 12 0.34769077955909466 1103 13 0.5622766471655325 1103 14 0.5615298750127806 1103 15 0.49473700274275245 1103 16 0.4252335073142035 1103 17 0.5893592774479686 1103 18 0.5168033731255974 1103 19 0.579932201641931 1103 20 0.7405039254949273 1103 21 0.7691117048888572 1103 22 0.7840445468602724 1103 23 0.6940904051135419 1103 24 0.6407142275833522 1103 25 0.6182637319151425 1104 1 1.2431606043175627 1104 2 3.835702906575687 1104 3 2.297824250409434 1104 4 1.5837833872102038 1104 5 1.1047990096512095 1104 6 0.7601310443212483 1104 7 0.7248654742211329 1104 8 0.6822018343024041 1104 9 0.7512817623834849 1104 10 0.7829606161192828 1104 11 0.7302065111235905 1104 12 0.8467366393078493 1104 13 0.759894984359903 1104 14 0.8369659600204429 1104 15 0.7037053399930002 1104 16 0.7607955146965537 1104 17 0.6535123019652858 1104 18 0.7854517414876554 1104 19 0.837603375274675 1104 20 0.6526750806885602 1104 21 0.8322825393536775 1104 22 0.8686419651610393 1104 23 1.0715533397038683 1104 24 1.1416597629154808 1104 25 1.0286780308321468 1105 1 1.0898951576276232 1105 2 3.499351591891614 1105 3 2.0231585960416787 1105 4 1.331695520708422 1105 5 0.9222688624504229 1105 6 0.5039965911974704 1105 7 0.1415725806638406 1105 8 0.14617260286686928 1105 9 0.3431100577034272 1105 10 0.39462136031552575 1105 11 0.0613958840722546 1105 12 0.12477314468503664 1105 13 0.19882896577326648 1105 14 0.24764688757845477 1105 15 0.3057192408398919 1105 16 0.1366388797033622 1105 17 0.26861042712508976 1105 18 0.202503890535624 1105 19 0.28582089430745583 1105 20 0.4541386092286004 1105 21 0.413604319956157 1105 22 0.4276533822229709 1105 23 0.3357372650233401 1105 24 0.33293338104920167 1105 25 0.37601591951057856 1106 1 0.5507161151345166 1106 2 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1107 14 0.4786192994363603 1107 15 0.47062101240788223 1107 16 0.5653210343297275 1107 17 0.40873779386395626 1107 18 0.33943639506122025 1107 19 0.568789271630564 1107 20 0.5660066946553428 1107 21 0.5562442882000198 1107 22 0.6605078925864873 1107 23 0.6406714429056066 1107 24 0.733251455696653 1107 25 0.9044309016990795 1108 1 0.5369371700273717 1108 2 2.2600718386763283 1108 3 1.388719799306255 1108 4 0.7785405566495385 1108 5 0.6266588846630654 1108 6 0.5398485128052499 1108 7 0.5793103706447056 1108 8 0.48836262810507236 1108 9 0.41846852334190743 1108 10 0.32016741057509535 1108 11 0.39099044663954174 1108 12 0.5222388613263647 1108 13 0.47012479983146704 1108 14 0.3373323177498264 1108 15 0.3048210281885133 1108 16 0.48093544998077675 1108 17 0.5421984368371326 1108 18 0.5747928641401501 1108 19 0.7085878431074448 1108 20 0.5233164263386385 1108 21 0.6243224866694632 1108 22 0.6888577183524234 1108 23 0.5958293932767589 1108 24 0.5817836688554436 1108 25 0.5209612829683863 1109 1 1.015653343266223 1109 2 3.431684605526822 1109 3 1.7745222824757931 1109 4 1.3323482722243014 1109 5 0.9938372426493487 1109 6 0.54956651522361 1109 7 0.5256923631034667 1109 8 0.37488741640252243 1109 9 0.3675833219123916 1109 10 0.3918028817638728 1109 11 0.4676867522183059 1109 12 0.45072049885175147 1109 13 0.5740896522628702 1109 14 0.5628950180484225 1109 15 0.40001010318831476 1109 16 0.5208897300920938 1109 17 0.5806678916207915 1109 18 0.4947755817635553 1109 19 0.4399197390045728 1109 20 0.4328789944731284 1109 21 0.5559828325978984 1109 22 0.5869956695885179 1109 23 0.5802955639642775 1109 24 0.5970431667106553 1109 25 0.5271903752990568 1110 1 0.06408485498788963 1110 2 1.7221459292175112 1110 3 0.7578019196713797 1110 4 0.2757326636431685 1110 5 0.20978309074008905 1110 6 0.28879911117880397 1110 7 0.09736550071628614 1110 8 0.05888497783693225 1110 9 0.039142556228128456 1110 10 0.04061163693076253 1110 11 -0.00825329136595343 1110 12 -0.08396419800131127 1110 13 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-2.208906445804665 2315 23 -1.9987095410890845 2315 24 -1.9435500416517968 2315 25 -2.0280092483557084 2316 1 -1.671820487083206 2316 2 -1.9705877977300688 2316 3 -1.3408869413542526 2316 4 -1.828709715087708 2316 5 -1.8845435860157664 2316 6 -1.8894816129694583 2316 7 -1.9114292856484063 2316 8 -2.080553385589557 2316 9 -2.3514958171703455 2316 10 -2.5442053311418746 2316 11 -2.724453642264397 2316 12 -2.563461029617784 2316 13 -2.6721293042012206 2316 14 -3.122920523510878 2316 15 -3.2458719908405484 2316 16 -3.089798636209345 2316 17 -3.010265635571166 2316 18 -2.814580321646247 2316 19 -3.020105016911515 2316 20 -3.058035146232328 2316 21 -3.200055224825608 2316 22 -3.029756332878275 2316 23 -3.0178849145833233 2316 24 -2.9905492572638472 2316 25 -3.387893537483656 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 119034.0 3 6.0 4 1007.0 5 4881.0 6 5943.0 7 5315.0 8 4999.0 9 4922.0 10 4621.0 11 4602.0 12 4570.0 13 4661.0 14 4615.0 15 4880.0 16 5260.0 17 5560.0 18 6535.0 19 7782.0 20 10407.0 21 13296.0 22 16401.0 23 19806.0 24 23035.0 25 26533.0 26 30244.0 27 34329.0 28 40046.0 29 48265.0 30 60472.0 31 76530.0 32 98933.0 33 127825.0 34 171590.0 35 232526.0 36 331017.0 37 458583.0 38 944833.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.60445148630301 19.133637710772152 13.170341149256512 25.091569653668323 2 15.928680655624362 21.805594995158483 39.59839215762358 22.667332191593573 3 18.40377966060521 25.113837881900114 30.11457340822656 26.367809049268114 4 12.42226363962719 17.460484016810486 38.478756110266865 31.638496233295456 5 13.185996388498259 37.93169997003911 35.725863264981115 13.156440376481513 6 30.968020125079963 37.423005913901584 18.650552117101192 12.958421843917264 7 27.333777798171578 31.98095459170866 22.817612414064882 17.86765519605488 8 24.793739770494692 36.91697935086524 21.09153186747116 17.19774901116891 9 26.262912198400468 15.663168080586692 20.75142449181204 37.322495229200804 10 15.207985251684963 28.94812312575746 34.48319491042774 21.360696712129844 11 33.45173732667895 22.75212357921956 25.271402466509933 18.524736627591547 12 23.218271823538462 24.74044018214061 31.999477708828746 20.041810285492183 13 28.558159213783092 22.32096344501637 26.1556873054904 22.96519003571014 14 21.038075476154326 22.353695362450864 27.87654570912833 28.731683452266477 15 22.711939549183093 30.975274169125168 23.81526277858903 22.497523503102705 16 22.025167146670697 28.218265075590516 28.011710388870743 21.744857388868045 17 21.54876202146927 28.64908781239625 28.089784146641005 21.712366019493473 18 21.317172447858606 27.759168436878344 30.001005444244406 20.922653671018644 19 23.303517414662604 26.98565009803118 27.8438573033143 21.86697518399192 20 23.30827701876305 27.594482459848773 28.27997437123316 20.817266150155017 21 22.81521394397846 26.953229909644854 27.479266901945437 22.75228924443125 22 23.057722414165408 28.02971820341179 27.638097873029583 21.27446150939322 23 22.322497405396504 27.783277392467348 27.928932831822458 21.965292370313687 24 22.947133733892606 27.030867896416023 28.483603791817146 21.53839457787423 25 22.669764874501663 27.459053451845293 28.16799286337025 21.703188810282793 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 886.0 1 886.0 2 941.5 3 997.0 4 997.0 5 997.0 6 2253.5 7 3510.0 8 3510.0 9 3510.0 10 5080.5 11 6651.0 12 6651.0 13 6651.0 14 10996.5 15 15342.0 16 15342.0 17 15342.0 18 26759.0 19 38176.0 20 38176.0 21 38176.0 22 59438.0 23 80700.0 24 80700.0 25 80700.0 26 114895.5 27 149091.0 28 149091.0 29 149091.0 30 191445.0 31 233799.0 32 233799.0 33 233799.0 34 262222.5 35 290646.0 36 290646.0 37 290646.0 38 312490.0 39 334334.0 40 334334.0 41 334334.0 42 351775.0 43 369216.0 44 369216.0 45 369216.0 46 373243.0 47 377270.0 48 377270.0 49 377270.0 50 364641.0 51 352012.0 52 352012.0 53 352012.0 54 314712.0 55 277412.0 56 277412.0 57 277412.0 58 236282.0 59 195152.0 60 195152.0 61 195152.0 62 159154.5 63 123157.0 64 123157.0 65 123157.0 66 95146.5 67 67136.0 68 67136.0 69 67136.0 70 48810.5 71 30485.0 72 30485.0 73 30485.0 74 21203.0 75 11921.0 76 11921.0 77 11921.0 78 8057.0 79 4193.0 80 4193.0 81 4193.0 82 2722.0 83 1251.0 84 1251.0 85 1251.0 86 818.0 87 385.0 88 385.0 89 385.0 90 242.5 91 100.0 92 100.0 93 100.0 94 64.5 95 29.0 96 29.0 97 29.0 98 21.0 99 13.0 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.352730084781218 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 3.711371371965785E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 4.7235635643200896E-4 15 0.0 16 0.0 17 0.0 18 0.0 19 3.711371371965785E-4 20 2.3617817821600448E-4 21 1.34958958980574E-4 22 8.09753753883444E-4 23 6.7479479490287E-4 24 2.69917917961148E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2963864.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.44807343376644 #Duplication Level Percentage of deduplicated Percentage of total 1 80.94943272804174 56.217821485187805 2 12.575821311168815 17.467331238159538 3 3.4512759246668967 7.190533915693706 4 1.2966707346571416 3.6020513759954027 5 0.6040114238883793 2.0973714860517 6 0.31184243578169274 1.2994113827958957 7 0.18849968394677064 0.9163657925083964 8 0.12195852943082894 0.67758279262291 9 0.09186932398974593 0.5742132802875322 >10 0.3744184576643763 4.323335029840492 >50 0.018282250694414606 0.8818126504151785 >100 0.012639460764443888 1.7435147399042041 >500 0.0020546997439918183 1.0283208830132917 >1k 0.0011741141383452016 1.7497796967708246 >5k 4.892142243105006E-5 0.2305542507531128 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6786 0.22895787391054379 No Hit GTCCTACAGTGGACATTTCTAAATT 4248 0.1433264144373696 No Hit GGTATCAACGCAGAGTACTTTTTTT 4128 0.13927764566795237 No Hit CTTTAGGACGTGAAATATGGCGAGG 4079 0.13762439842044033 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4079 0.13762439842044033 No Hit TATCAACGCAGAGTACTTTTTTTTT 4060 0.13698334336528262 No Hit CTGTAGGACGTGGAATATGGCAAGA 4047 0.13654472674859575 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.37397397451435E-5 7 0.0 0.0 0.0 0.0 3.37397397451435E-5 8 0.0 0.0 0.0 0.0 3.37397397451435E-5 9 0.0 0.0 0.0 0.0 3.37397397451435E-5 10 3.37397397451435E-5 0.0 0.0 0.0 3.37397397451435E-5 11 3.37397397451435E-5 0.0 0.0 0.0 3.37397397451435E-5 12 3.37397397451435E-5 0.0 0.0 0.0 3.37397397451435E-5 13 3.37397397451435E-5 0.0 0.0 0.0 6.7479479490287E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAATCGC 40 0.005444205 14.1846 15 GTCGTAG 50 0.0011100138 13.841642 1 GGTATCA 1540 0.0 11.941307 1 TAGGACC 975 0.0 11.638843 4 TCCAACG 280 0.0 11.482771 18 TTAGGAC 1570 0.0 10.902147 3 CGGTAGG 130 2.220786E-7 10.647417 1 GACGTGA 1425 0.0 10.551529 7 AATCCCG 395 0.0 10.533711 19 AGGACGT 2660 0.0 10.523088 5 GGACGTG 2635 0.0 10.37171 6 CCAACGT 210 0.0 10.357009 19 GTATCAA 3195 0.0 10.335588 1 GTCCTAA 1390 0.0 10.171403 1 GGCGAGG 1280 0.0 10.121303 19 GTCCTAC 2415 0.0 10.0711155 1 GATATAC 730 0.0 9.8868885 1 GTACTAG 150 1.4826219E-7 9.8868885 1 GTACCGT 115 1.0846266E-5 9.867713 6 TAGGACT 375 0.0 9.834822 4 >>END_MODULE