FastQCFastQC Report
Thu 26 May 2016
SRR1512134_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512134_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2963864
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT85140.2872601441901518No Hit
TATCAACGCAGAGTACTTTTTTTTT56720.19137180383445393No Hit
GGTATCAACGCAGAGTACTTTTTTT53340.17996777180059542No Hit
GTCCTACAGTGGACATTTCTAAATT47040.15871173576115502No Hit
CTTTAGGACGTGAAATATGGCGAGG44110.148825992015828No Hit
GTCCTAAAGTGTGTATTTCTCATTT44050.14862355357735713No Hit
CTGTAGGACGTGGAATATGGCAAGA42530.1434951131360953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8950.012.6326434
GGTATCA18000.011.869971
GTATCAA38500.010.7275681
AGGACCT17750.010.4912685
AATCCCG3650.010.40803819
TAGACCG1106.0422844E-610.3647515
TGGCGAG27850.010.162147518
GGACGTA750.002655327310.130326
AAGACCG1151.0258353E-59.9141085
GGCGAGG13350.09.8886119
TGTAGGA28250.09.753262
TAGAAAT8900.09.7145284
AGGACGT27550.09.6217245
ATAGGAC4350.09.6102283
ATGGCGA30350.09.512517
GACGTGA16000.09.4971747
GACGTAG800.00452858779.4971747
GGACGTG27100.09.4270846
GTCCTAC26450.09.4121521
TTTAGAA10100.09.4069522
Produced by FastQC (version 0.11.5)