FastQCFastQC Report
Thu 26 May 2016
SRR1512133_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512133_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2888127
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61980.21460275119480546No Hit
TATCAACGCAGAGTACTTTTTTTTT39160.13558960530475286No Hit
GGTATCAACGCAGAGTACTTTTTTT37720.1306036749768968No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCCC400.005449390514.1825474
GGTATCA15200.011.658091
CGGTTAT600.00432330911.54954051
TAGGACC5350.010.9572324
GTATCAA31600.010.5574921
AGGACGT13500.010.50558955
GTCCTAA7350.010.37101651
GGACGTG13550.09.9783736
CGTGCGC1054.3264554E-59.90544210
GTCTACG800.0032137449.8996071
TTAGGAC9250.09.8127893
TGGCGAG13750.09.76446718
TAGGACG15000.09.7071664
GTAGGAC15150.09.6110553
GACGTGG7000.09.5901027
GATATAC5750.09.469191
TGTAGGA15350.09.4159122
GGCGAGG7150.09.38891119
TATACTG4950.09.3595265
TATGGCG14700.09.32639116
Produced by FastQC (version 0.11.5)