FastQCFastQC Report
Thu 26 May 2016
SRR1512133_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512133_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2888127
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT80320.2781041138426392No Hit
TATCAACGCAGAGTACTTTTTTTTT52540.18191720793441563No Hit
GGTATCAACGCAGAGTACTTTTTTT49830.1725339640535198No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG655.444369E-513.1567485
GGTATCA17550.012.7765021
TAGGACC6750.011.4025154
GGACCGC855.346308E-511.1723576
ACCGGGT700.001496663410.8531468
GTCCTAC14550.010.8203711
AGGACGT13400.010.6366755
GTAGGAC14550.010.5796533
GGACGTG13800.010.3911016
TGTAGGA16050.010.360542
TAGGACG14800.010.33674054
GGCGAGG6800.010.05546919
TCCTACA17500.09.8278832
TTAGGAC9950.09.7408433
GTATCAA35200.09.7313481
GACGTGG7350.09.6903097
GTCCTAA9500.09.5415991
TGGACCG1804.1709427E-99.5020965
CTAGTAC1201.6970062E-59.5020963
ACCGTCC900.00111788129.4965038
Produced by FastQC (version 0.11.5)