FastQCFastQC Report
Thu 26 May 2016
SRR1512132_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512132_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2400799
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68750.2863629983184765No Hit
GGTATCAACGCAGAGTACTTTTTTT41300.17202606298986295No Hit
TATCAACGCAGAGTACTTTTTTTTT41240.17177614619133047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16350.012.3941441
GGATCGT701.1389602E-412.1585626
GTATCAA33350.010.3741521
GTCCTAC2103.6379788E-1210.3558031
ACCGAGC1054.318279E-59.9069778
CTCAACG800.00325268069.8850852
GTATAGG2001.2369128E-109.8850851
GTATTAC1251.7229771E-59.4896821
ACAACGC900.00116154149.4566598
AGGATCG900.00116154149.4566595
CCTATTC1752.8770955E-89.1864693
TAGGACT3000.09.1414384
TTACACT3350.09.0332274
CTCGAAA850.0077213798.90038518
GTATTAT2251.1605152E-98.7867421
CTTACAC2701.8189894E-128.75616553
CCTAGAC1951.7831553E-88.7292243
GACGCTC1201.8538965E-48.6686048
GTATAAC1501.5698177E-58.5670741
GTATAGA3250.08.5163811
Produced by FastQC (version 0.11.5)