FastQCFastQC Report
Thu 26 May 2016
SRR1512132_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512132_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2400799
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT89770.37391718340435826No Hit
TATCAACGCAGAGTACTTTTTTTTT60200.25074985452759685No Hit
GGTATCAACGCAGAGTACTTTTTTT56020.23333898422983348No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27400.11412867132983644No Hit
GGTATCAACGCAGAGTACATGGGGG24180.10071646980859289No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20250.013.0908961
ATTGCGA1303.8619692E-710.22722510
CTAATAC2450.010.0836013
TGCACCG856.5839064E-410.0607875
GTCTTAT1752.413799E-99.8080811
TGCGAGC1554.087633E-89.80305612
TAGGACC1951.6370905E-109.7454914
GTGTAGC3850.09.6594731
TTGCGAG1201.7080518E-59.4967111
GTATCAA40300.09.4173261
GTATTAA2950.09.3740131
ATAGGAC2553.6379788E-128.9429233
GTATAAA3200.08.9396571
CGTCCAA850.0074583878.93807910
CATTGCG1606.776809E-78.9031659
ACAATAC2054.127287E-98.8065973
TAGACTG2701.8189894E-128.7980145
GTATTAT2409.458745E-118.7409981
GACTGCG1107.166119E-48.6339127
TTTATAC2554.0017767E-118.5703013
Produced by FastQC (version 0.11.5)