FastQCFastQC Report
Thu 26 May 2016
SRR1512131_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512131_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2852168
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43290.15177927807899114No Hit
GTCCTACAGTGGACATTTCTAAATT33210.11643774139531753No Hit
GTCCTAAAGTGTGTATTTCTCATTT32440.1137380406764258No Hit
CTGTAGGACGTGGAATATGGCAAGA30280.10616485424421003No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG350.002242960316.2061655
GGCGTAC450.00868493613.2181651
TAGGACC6950.011.9700214
GGTATCA13650.011.6929921
ACCGTAT658.3635334E-411.6351968
CGGTAGG1301.744047E-811.4387971
CCTTACG650.00776243510.676211
GTATCAA29300.010.5226531
AATACCG901.00049576E-410.5039965
GTAGGAC20700.010.4126573
GTCCTAA11650.010.3816491
AGGACGT21250.010.3211035
GTATAAG3100.010.2334181
GCCGGTT1304.111953E-710.18079611
GGCGAGG10500.010.08383719
GGACGTG21250.09.9652026
TAGGACG22550.09.9357094
GTCTAGA2400.09.9136241
GACGTGA11850.09.8923717
GTCCTAC20250.09.840191
Produced by FastQC (version 0.11.5)