FastQCFastQC Report
Thu 26 May 2016
SRR1512131_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512131_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2852168
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50480.1769881718047464No Hit
GTCCTACAGTGGACATTTCTAAATT35260.12362525629626304No Hit
CTGTAGGACGTGGAATATGGCAAGA35190.12337982895818198No Hit
CTTTAGGACGTGAAATATGGCGAGG33980.11913744211420926No Hit
GTCCTAAAGTGTGTATTTCTCATTT32800.11500023841512841No Hit
TATCAACGCAGAGTACTTTTTTTTT30660.10749717407950724No Hit
GGTATCAACGCAGAGTACTTTTTTT29560.10364045876680476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7850.012.4686854
CTAATAC2400.011.4825913
GGTATCA13450.011.3472841
TACACCG1408.3218765E-910.8603815
GTATCAA29400.010.3499341
CTAGTAC1856.002665E-1110.2733343
GTAGGAC22250.010.1648283
TGTAGGA23650.09.8845542
AGGACCT16250.09.8244685
TATACTG4400.09.7188075
AGGACGT22500.09.7140075
GGACGTG22400.09.53889756
GTCTAAT1803.9017323E-99.5388111
GTCCTAC21300.09.4940281
GTCCTAA13050.09.4291691
TAGGACG23300.09.4212644
GACGTGG11450.09.3720957
CTGTAGG24250.09.2831321
TGGCGAG21400.09.23073118
TAGGACT3950.09.1419664
Produced by FastQC (version 0.11.5)