FastQCFastQC Report
Thu 26 May 2016
SRR1512130_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512130_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2627987
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55440.210959947670974No Hit
GTCCTACAGTGGACATTTCTAAATT41640.15844827238490905No Hit
CTGTAGGACGTGGAATATGGCAAGA39310.14958217068805896No Hit
GTCCTAAAGTGTGTATTTCTCATTT39160.1490113916088626No Hit
TATCAACGCAGAGTACTTTTTTTTT37450.1425045101060241No Hit
CTTTAGGACGTGAAATATGGCGAGG37330.14204788684266703No Hit
GGTATCAACGCAGAGTACTTTTTTT36090.1373294464546438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1400.014.18161919
CGATATT500.001093880313.8683961
TCCAACG2200.012.46263618
TAGGACC8650.012.0229524
GGTATCA16450.011.5017961
GGACGTA752.1696264E-411.3452956
ATACCGT752.1696264E-411.3452956
GGCGAGG10900.011.27590619
GTATTAT3050.011.0427491
CCTACAC3250.010.4725813
GTCCTAG3450.010.3366911
TGGCGAG23200.010.26944818
TGTAGGA22500.010.258212
GGCGAAA1201.6217582E-610.24228119
CGGTTTC1951.6370905E-1110.18167613
CTACACT3550.010.1202174
GTAGGAC23500.010.0578863
GTATCAA32150.010.0446491
AGCGCCC951.7265831E-49.9520148
CGTTAGG907.592037E-49.9059951
Produced by FastQC (version 0.11.5)