##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512130_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2627987 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.35542146897987 31.0 28.0 33.0 16.0 34.0 2 25.83117306135837 31.0 25.0 33.0 7.0 34.0 3 27.484537785004264 31.0 26.0 34.0 16.0 34.0 4 31.706303722202584 35.0 32.0 37.0 19.0 37.0 5 32.102434677188285 35.0 33.0 37.0 19.0 37.0 6 32.345267689680355 35.0 33.0 37.0 22.0 37.0 7 32.36991735499453 35.0 33.0 37.0 22.0 37.0 8 32.4033357090427 36.0 33.0 37.0 23.0 37.0 9 33.88988187536696 38.0 34.0 39.0 23.0 39.0 10 33.71805073617183 38.0 34.0 39.0 22.0 39.0 11 33.73439176068984 38.0 34.0 39.0 22.0 39.0 12 33.616695592482 38.0 34.0 39.0 22.0 39.0 13 33.60262550766043 38.0 34.0 39.0 21.0 39.0 14 34.59784428157369 39.0 34.0 41.0 19.0 41.0 15 34.529405967381116 39.0 34.0 41.0 19.0 41.0 16 34.486108188510826 39.0 34.0 41.0 18.0 41.0 17 34.42443893367813 39.0 34.0 41.0 18.0 41.0 18 34.36913538765603 39.0 34.0 41.0 17.0 41.0 19 34.38281544010682 39.0 34.0 41.0 18.0 41.0 20 34.305610339777175 39.0 34.0 41.0 18.0 41.0 21 34.223471425086956 39.0 34.0 41.0 17.0 41.0 22 34.09731973559991 39.0 34.0 41.0 11.0 41.0 23 33.97156911354584 39.0 34.0 41.0 10.0 41.0 24 33.84370432578243 39.0 34.0 41.0 2.0 41.0 25 33.7109399704032 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.009528427786936 1101 2 3.2483062580512083 1101 3 1.9153606054591634 1101 4 1.2870950452713856 1101 5 0.8792301409625836 1101 6 0.5819675432093732 1101 7 0.4069258572194556 1101 8 0.30021441312918284 1101 9 0.3782748727023275 1101 10 0.22059854296204406 1101 11 0.5452810645634543 1101 12 0.31369344680765465 1101 13 0.2652902004753628 1101 14 0.29863321692875644 1101 15 0.36270709432604065 1101 16 0.28863148916624226 1101 17 0.24923199111872663 1101 18 0.28977583296638443 1101 19 0.28697932135784043 1101 20 0.4331270264691156 1101 21 0.4520458158573035 1101 22 0.27821784119731774 1101 23 0.25073264661251926 1101 24 0.20517471278387234 1101 25 0.21046049352403173 1102 1 1.4857193788972403 1102 2 4.17912285823741 1102 3 2.712827150384882 1102 4 1.8634835844016067 1102 5 1.5529795244869646 1102 6 1.336992474007026 1102 7 1.1892216581374342 1102 8 1.2031841841734163 1102 9 1.2625426808482985 1102 10 1.2122676655578744 1102 11 1.3884516616310023 1102 12 1.260748268299423 1102 13 1.3865261669803104 1102 14 1.285805038719559 1102 15 1.4124177601064076 1102 16 1.5563995756508504 1102 17 1.3130542347060086 1102 18 1.2829318108143184 1102 19 1.343303958125503 1102 20 1.4426655062664224 1102 21 1.3513789833011458 1102 22 1.4840632684234407 1102 23 1.3989125246820748 1102 24 1.363464707901933 1102 25 1.3008017853976241 1103 1 1.1105431743632863 1103 2 2.85748837083133 1103 3 1.9203701513257592 1103 4 1.3057005679846299 1103 5 1.0723947580553954 1103 6 0.837491530332521 1103 7 0.7583858447107943 1103 8 0.6720413268021659 1103 9 0.8338514586599217 1103 10 0.731690733103548 1103 11 0.7739365808251293 1103 12 0.7359787511042413 1103 13 0.8394296547788898 1103 14 0.823304289295713 1103 15 0.6602425923753614 1103 16 0.6936494179720754 1103 17 0.8443067677586029 1103 18 0.748610916717702 1103 19 0.8789078658412492 1103 20 0.8627687708765919 1103 21 0.9599957187549251 1103 22 0.8075010094881137 1103 23 0.8506054231817046 1103 24 0.71683447722328 1103 25 0.8005737207337376 1104 1 1.101567872569035 1104 2 3.6627743664563255 1104 3 2.174049330171183 1104 4 1.5712816894065682 1104 5 1.160743817304919 1104 6 0.8607705085011261 1104 7 0.7747444329930175 1104 8 0.8647440446642705 1104 9 0.8435394124419275 1104 10 0.7772403195971194 1104 11 0.7878323597565569 1104 12 0.7728158788082382 1104 13 0.6696290228338881 1104 14 0.8033919451786034 1104 15 0.52892532357437 1104 16 0.6297302682933434 1104 17 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-2.8038653863817373 2315 23 -2.8103315093774803 2315 24 -2.8027478027792156 2315 25 -2.8791274450488054 2316 1 -1.6739035481221585 2316 2 -2.002545052174476 2316 3 -1.3478471542826505 2316 4 -1.956881024008478 2316 5 -1.839459194867164 2316 6 -1.9499565585712126 2316 7 -1.899011402705991 2316 8 -2.1738940968301677 2316 9 -2.276836955307534 2316 10 -2.4690136853885676 2316 11 -2.730917556155564 2316 12 -2.6526964326186793 2316 13 -2.5312881597319574 2316 14 -2.8504804699599084 2316 15 -3.407307472044984 2316 16 -2.9956051222299998 2316 17 -3.0277906294644374 2316 18 -2.7468616190060295 2316 19 -2.980238055686602 2316 20 -2.9499751213799286 2316 21 -3.154849727269113 2316 22 -3.028061336850776 2316 23 -2.868595130699177 2316 24 -2.9054013292743406 2316 25 -3.294560684362356 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 141209.0 3 2.0 4 1215.0 5 5734.0 6 6691.0 7 6039.0 8 5437.0 9 5391.0 10 5115.0 11 4960.0 12 4984.0 13 4819.0 14 4903.0 15 5061.0 16 5418.0 17 5814.0 18 6691.0 19 8417.0 20 10823.0 21 14042.0 22 17153.0 23 20088.0 24 23475.0 25 26433.0 26 29513.0 27 33340.0 28 38123.0 29 45874.0 30 56014.0 31 70478.0 32 88308.0 33 114137.0 34 150013.0 35 199268.0 36 282293.0 37 386923.0 38 793789.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.72087342897815 19.35116117393275 12.979934832249931 24.948030564839172 2 15.79722082459874 21.918080747109713 39.93479853888765 22.349899889403904 3 18.222464570791256 25.52828457675019 30.324997802500548 25.924253049958008 4 12.044808440833231 17.54681434877722 39.17108417964016 31.23729303074939 5 12.750595798228833 38.41815047030293 36.0635345608635 12.767719170604725 6 30.39117012374871 37.917158646522985 18.959949193051564 12.73172203667674 7 27.076846270548522 32.151414752051664 23.099733750585525 17.67200522681429 8 24.58452848539828 37.23851569533687 21.30729404888473 16.86966177038012 9 26.45161486719683 15.45224538781965 20.77426562612372 37.321874118859796 10 14.972829013233321 29.235875215516664 34.93628393138931 20.855011839860698 11 33.51660415367351 22.700112291270848 25.32044488804549 18.462838667010146 12 23.021042341533654 25.037566776395774 32.33885860165975 19.602532280410824 13 28.72369612178447 22.3677286074855 26.18719194577446 22.721383324955564 14 20.751833636164726 22.53891304141021 28.242163643109237 28.467089679315823 15 22.58245569707917 31.322301061611036 23.931054453465713 22.16418878784408 16 21.659163458571143 28.300482460529675 28.46330670585509 21.577047375044092 17 21.29219866468086 28.847794470746802 28.374808693805825 21.485198170766513 18 20.94709753130438 27.8517359484655 30.435120112846832 20.766046407383293 19 23.099806962221596 27.180921642187183 28.069702125165954 21.649569270425264 20 23.12631160375725 27.859215330376696 28.51953112365558 20.494941942210477 21 22.747115091176283 27.135380477673777 27.581653783543747 22.53585064760619 22 22.87152331589656 28.349285437320276 27.791098151387516 20.988093095395648 23 22.108974649244338 28.019204183915413 28.158361076557597 21.71346009028265 24 22.68684590946434 27.119259994649887 28.83654790502671 21.357346190859065 25 22.419830242626862 27.63785651826151 28.482457669104782 21.45985557000684 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 709.0 1 709.0 2 778.5 3 848.0 4 848.0 5 848.0 6 2033.5 7 3219.0 8 3219.0 9 3219.0 10 4633.0 11 6047.0 12 6047.0 13 6047.0 14 10013.5 15 13980.0 16 13980.0 17 13980.0 18 24703.5 19 35427.0 20 35427.0 21 35427.0 22 55312.0 23 75197.0 24 75197.0 25 75197.0 26 106688.0 27 138179.0 28 138179.0 29 138179.0 30 177252.5 31 216326.0 32 216326.0 33 216326.0 34 241294.0 35 266262.0 36 266262.0 37 266262.0 38 285504.5 39 304747.0 40 304747.0 41 304747.0 42 318886.5 43 333026.0 44 333026.0 45 333026.0 46 334877.5 47 336729.0 48 336729.0 49 336729.0 50 321451.5 51 306174.0 52 306174.0 53 306174.0 54 270843.0 55 235512.0 56 235512.0 57 235512.0 58 199285.5 59 163059.0 60 163059.0 61 163059.0 62 131835.0 63 100611.0 64 100611.0 65 100611.0 66 77006.5 67 53402.0 68 53402.0 69 53402.0 70 38742.5 71 24083.0 72 24083.0 73 24083.0 74 16902.0 75 9721.0 76 9721.0 77 9721.0 78 6530.5 79 3340.0 80 3340.0 81 3340.0 82 2176.5 83 1013.0 84 1013.0 85 1013.0 86 639.0 87 265.0 88 265.0 89 265.0 90 171.5 91 78.0 92 78.0 93 78.0 94 52.5 95 27.0 96 27.0 97 27.0 98 16.5 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.557138220242337 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 3.4246744751781496E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 4.566232633570866E-4 15 0.0 16 0.0 17 3.805193861309055E-5 18 0.0 19 1.522077544523622E-4 20 7.61038772261811E-5 21 3.805193861309055E-5 22 6.088310178094488E-4 23 6.849348950356299E-4 24 2.283116316785433E-4 25 3.805193861309055E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2627987.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.37892204860269 #Duplication Level Percentage of deduplicated Percentage of total 1 84.29721844036338 61.01341802409094 2 10.688928561678864 15.473062542976745 3 2.626008781106038 5.7020305459986025 4 0.9484246607133028 2.745838183469624 5 0.44810388595885076 1.6216638115745803 6 0.24336607262613869 1.0568744399929122 7 0.1645517156603449 0.8337053060520756 8 0.10247751595502111 0.5933769711234329 9 0.08044468853573618 0.5240249856676954 >10 0.3648532264292637 4.43993383068024 >50 0.01885259835456327 0.9370377107857248 >100 0.013529504854097605 1.9712737373926683 >500 0.0019654568407509693 1.0273693507879325 >1k 0.0012217704685295494 1.8472347762917027 >5k 5.312045515345867E-5 0.21315578311502134 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5544 0.210959947670974 No Hit GTCCTACAGTGGACATTTCTAAATT 4164 0.15844827238490905 No Hit CTGTAGGACGTGGAATATGGCAAGA 3931 0.14958217068805896 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3916 0.1490113916088626 No Hit TATCAACGCAGAGTACTTTTTTTTT 3745 0.1425045101060241 No Hit CTTTAGGACGTGAAATATGGCGAGG 3733 0.14204788684266703 No Hit GGTATCAACGCAGAGTACTTTTTTT 3609 0.1373294464546438 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.805193861309055E-5 0.0 5 0.0 3.805193861309055E-5 0.0 3.805193861309055E-5 0.0 6 7.61038772261811E-5 3.805193861309055E-5 0.0 3.805193861309055E-5 0.0 7 7.61038772261811E-5 3.805193861309055E-5 0.0 3.805193861309055E-5 0.0 8 7.61038772261811E-5 3.805193861309055E-5 0.0 3.805193861309055E-5 0.0 9 7.61038772261811E-5 3.805193861309055E-5 0.0 7.61038772261811E-5 3.805193861309055E-5 10 7.61038772261811E-5 3.805193861309055E-5 0.0 7.61038772261811E-5 3.805193861309055E-5 11 7.61038772261811E-5 3.805193861309055E-5 0.0 7.61038772261811E-5 7.61038772261811E-5 12 7.61038772261811E-5 3.805193861309055E-5 0.0 7.61038772261811E-5 7.61038772261811E-5 13 7.61038772261811E-5 3.805193861309055E-5 0.0 7.61038772261811E-5 7.61038772261811E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACGA 140 0.0 14.181619 19 CGATATT 50 0.0010938803 13.868396 1 TCCAACG 220 0.0 12.462636 18 TAGGACC 865 0.0 12.022952 4 GGTATCA 1645 0.0 11.501796 1 GGACGTA 75 2.1696264E-4 11.345295 6 ATACCGT 75 2.1696264E-4 11.345295 6 GGCGAGG 1090 0.0 11.275906 19 GTATTAT 305 0.0 11.042749 1 CCTACAC 325 0.0 10.472581 3 GTCCTAG 345 0.0 10.336691 1 TGGCGAG 2320 0.0 10.269448 18 TGTAGGA 2250 0.0 10.25821 2 GGCGAAA 120 1.6217582E-6 10.242281 19 CGGTTTC 195 1.6370905E-11 10.181676 13 CTACACT 355 0.0 10.120217 4 GTAGGAC 2350 0.0 10.057886 3 GTATCAA 3215 0.0 10.044649 1 AGCGCCC 95 1.7265831E-4 9.952014 8 CGTTAGG 90 7.592037E-4 9.905995 1 >>END_MODULE