FastQCFastQC Report
Thu 26 May 2016
SRR1512130_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512130_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2627987
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT71150.2707395432321393No Hit
TATCAACGCAGAGTACTTTTTTTTT48870.1859598240021735No Hit
GTCCTACAGTGGACATTTCTAAATT48200.18341034411509646No Hit
GGTATCAACGCAGAGTACTTTTTTT47000.1788441114815256No Hit
GTCCTAAAGTGTGTATTTCTCATTT43160.16423216705409882No Hit
CTGTAGGACGTGGAATATGGCAAGA42160.16042697319278976No Hit
CTTTAGGACGTGAAATATGGCGAGG40800.15525190954140944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGTT1251.03682396E-1012.91496711
TAGGACC7600.012.3781864
GGTATCA20400.012.345941
TACCGTC550.003077507512.0866597
TATACTG3400.012.0177995
CCGGTTT1451.2259989E-911.13359212
GGCGAGG11300.011.00961319
CGGTTTC1502.1482265E-910.76247313
TGGCGAG24600.010.23033718
ACCGATC750.002656347610.1297718
TGTAGGA26200.09.9014032
TTAGGAC16800.09.8983813
GCGCCAC1252.5843601E-69.87615113
TTTATAC3800.09.7525113
GGACGTG25050.09.705176
AGGACGT25500.09.65150455
GACGTGA13850.09.5995127
GTAGGAC24900.09.5787713
CTACACG1201.6960797E-59.5024474
TGTCGAG1201.707966E-59.49684218
Produced by FastQC (version 0.11.5)