FastQCFastQC Report
Thu 26 May 2016
SRR1512129_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512129_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2989687
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51110.17095435073972626No Hit
GTCCTACAGTGGACATTTCTAAATT32970.11027910279571072No Hit
GGTATCAACGCAGAGTACTTTTTTT32720.10944289485822428No Hit
TATCAACGCAGAGTACTTTTTTTTT31460.10522840685329267No Hit
CTTTAGGACGTGAAATATGGCGAGG31180.10429185396330785No Hit
GTCCTAAAGTGTGTATTTCTCATTT30920.10342219770832198No Hit
CTGTAGGACGTGGAATATGGCAAGA30590.10231840323083989No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG1152.8885552E-912.9121651
GTCAACG904.718151E-612.0991762
TAGGACC6100.011.6251584
ATAGCGC600.00608858811.0309833
CAATACG951.4345658E-510.9480444
GGTATCA16400.010.9254761
GGCGAGG11200.010.13049419
GTATCAA31200.09.9627831
GACGTGA12350.09.8762077
GGACGTG20500.09.8702326
AGGACGT20700.09.8205445
GTCCTAA11900.09.6497631
GTATTAA3400.09.3170131
TTAGGAC14650.09.2292383
GTAGGAC20100.09.2199263
AATGTCC8000.09.218758
TGGCGAG21450.09.21268918
TAGGACG23100.09.1276784
GTATAGG2404.3655746E-119.0743821
ACGTGAA14800.09.0718138
Produced by FastQC (version 0.11.5)