FastQCFastQC Report
Thu 26 May 2016
SRR1512129_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512129_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2989687
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62620.2094533641816016No Hit
GGTATCAACGCAGAGTACTTTTTTT40920.1368705152077793No Hit
TATCAACGCAGAGTACTTTTTTTTT40550.13563292746029937No Hit
GTCCTACAGTGGACATTTCTAAATT35400.11840704394807886No Hit
CTTTAGGACGTGAAATATGGCGAGG34740.11619945499311465No Hit
GTCCTAAAGTGTGTATTTCTCATTT33410.11175082876568684No Hit
CTGTAGGACGTGGAATATGGCAAGA32810.1097439297157194No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGTA400.00529126514.2456727
GGTATCA17800.012.4312811
GTTCGCA550.003078117512.0864299
TACACCG1052.715915E-711.7642365
CCGGTAC600.00586914811.0855293
GGGCGTC600.00589404811.07922611
TTAGGAC12800.010.318453
GGACGTG19100.010.2429616
TGGCGAG20100.010.15911618
GGCGAGG10100.010.15584119
AGGACGT19650.010.1063285
TAGGACC6550.09.864554
CGCTCAC1356.481132E-79.84820210
ACACCGT1451.6258309E-79.8246026
TGTAGGA21450.09.6569252
GTCCTAC17100.09.5377941
TAGGACG21550.09.5239294
GACGTGA10600.09.4075197
ATGGCGA22050.09.34574217
TATGGCG21850.09.300899516
Produced by FastQC (version 0.11.5)