##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512129_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2989687 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.97508802760958 31.0 31.0 34.0 28.0 34.0 2 31.165553785396263 31.0 31.0 34.0 28.0 34.0 3 31.26995468087462 33.0 31.0 34.0 28.0 34.0 4 34.84791150377949 37.0 35.0 37.0 32.0 37.0 5 34.466331425329805 37.0 35.0 37.0 30.0 37.0 6 34.41205918880471 37.0 35.0 37.0 30.0 37.0 7 34.24681145551357 37.0 35.0 37.0 30.0 37.0 8 34.152694914216774 37.0 35.0 37.0 30.0 37.0 9 35.671024090481716 39.0 35.0 39.0 30.0 39.0 10 35.49239167845999 39.0 35.0 39.0 30.0 39.0 11 35.47891969962073 39.0 35.0 39.0 30.0 39.0 12 35.30993545478172 39.0 35.0 39.0 30.0 39.0 13 35.2820766856196 39.0 35.0 39.0 30.0 39.0 14 36.33515013444551 40.0 36.0 41.0 29.0 41.0 15 36.268795696673266 40.0 36.0 41.0 29.0 41.0 16 36.171316596018244 40.0 36.0 41.0 29.0 41.0 17 36.10697741937534 39.0 36.0 41.0 28.0 41.0 18 36.033107479144135 39.0 36.0 41.0 28.0 41.0 19 36.04340186782095 39.0 36.0 41.0 28.0 41.0 20 35.942575594033755 39.0 36.0 41.0 27.0 41.0 21 35.826589873789466 39.0 36.0 41.0 27.0 41.0 22 35.71357670552135 39.0 36.0 41.0 27.0 41.0 23 35.77692681541579 39.0 36.0 41.0 27.0 41.0 24 35.67090233860601 39.0 36.0 41.0 27.0 41.0 25 35.248946127136385 39.0 35.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05878376610451497 1101 2 0.07783644815846458 1101 3 0.06891026202125161 1101 4 0.020396612232829625 1101 5 0.09484238626064467 1101 6 -0.04339094975966162 1101 7 0.023971064821935784 1101 8 -0.06492694303378954 1101 9 -0.0949063948141955 1101 10 -0.05691538318036038 1101 11 -0.10314992792973499 1101 12 -0.1348099784416661 1101 13 -0.08613628447162824 1101 14 -0.04548615064271644 1101 15 -0.15879789353909501 1101 16 -0.30795712268935915 1101 17 -0.35810704915250824 1101 18 -0.2271498730135093 1101 19 -0.3229563844729455 1101 20 -0.3492614655191062 1101 21 -0.24843835186923258 1101 22 -0.1928193825377278 1101 23 -0.3962044616255227 1101 24 -0.4761505774663206 1101 25 -0.5270903185083 1102 1 0.04654139899060539 1102 2 0.11682316456340303 1102 3 0.120140181196561 1102 4 0.12962192526973837 1102 5 0.021456707406763087 1102 6 0.0874945786760506 1102 7 0.2298584317018637 1102 8 0.2642339576033095 1102 9 0.4177813771919858 1102 10 0.35976298267672036 1102 11 0.33447918099307117 1102 12 0.4343564709543202 1102 13 0.4365334039287916 1102 14 0.5291337928000601 1102 15 0.4886536885257087 1102 16 0.31502309565095743 1102 17 0.5298096927232407 1102 18 0.3858835265038181 1102 19 0.33164758121834126 1102 20 0.4701414746545751 1102 21 0.5784737972386225 1102 22 0.5419415862889423 1102 23 0.5964601459836345 1102 24 0.486940983740503 1102 25 0.44991325141609195 1103 1 0.276944300331774 1103 2 0.3628856102927891 1103 3 0.3792402963416279 1103 4 0.3619793783107639 1103 5 0.46761944823875723 1103 6 0.45180115693376166 1103 7 0.47287247070315175 1103 8 0.5441518988150236 1103 9 0.6087254649853548 1103 10 0.5607687325283379 1103 11 0.46816889810813933 1103 12 0.43646703982832946 1103 13 0.43675441862100683 1103 14 0.6337423250080647 1103 15 0.5374819752605262 1103 16 0.6348546017290388 1103 17 0.5534372157719147 1103 18 0.6874278305238093 1103 19 0.5673576108673899 1103 20 0.6513570994102551 1103 21 0.7003566342391849 1103 22 0.6326531402570055 1103 23 0.5214180826432013 1103 24 0.7420284852035266 1103 25 0.7686934631183249 1104 1 0.06205827590843782 1104 2 0.10496249857862949 1104 3 0.1659494777075281 1104 4 0.12560669626644483 1104 5 0.20464070558837477 1104 6 0.10121409225714473 1104 7 0.09388423008804381 1104 8 0.1544400037449165 1104 9 0.1926594315163399 1104 10 -0.08157364648568688 1104 11 0.3552982513419707 1104 12 0.29482027365916963 1104 13 0.1753034914387399 1104 14 0.3135306560799762 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-0.5791915930232285 2315 4 -0.5571678250309375 2315 5 -0.5715320181318262 2315 6 -0.5585311207166725 2315 7 -0.6513025616274746 2315 8 -0.6690039196626643 2315 9 -0.7243519010354404 2315 10 -0.6968096761847846 2315 11 -0.7629381691347774 2315 12 -0.7945744117648204 2315 13 -0.9208504749543849 2315 14 -1.0059605514229517 2315 15 -1.0215840396505342 2315 16 -1.0530403845990506 2315 17 -1.217372091629052 2315 18 -1.0792532583301195 2315 19 -1.0911690036354997 2315 20 -1.1146741956756472 2315 21 -1.117485970916853 2315 22 -1.1504175448976284 2315 23 -1.1487781757536126 2315 24 -1.0432071187229184 2315 25 -1.0737681338313791 2316 1 -0.9862342410983658 2316 2 -1.0356089299927973 2316 3 -1.0901366900929261 2316 4 -0.9526926234614521 2316 5 -1.1556744871553875 2316 6 -1.2107745698743742 2316 7 -1.4068345907736344 2316 8 -1.334351379475038 2316 9 -1.5910548541979495 2316 10 -1.6121541453518944 2316 11 -1.6221575309709522 2316 12 -1.6073656673839096 2316 13 -1.717989025568066 2316 14 -1.7874829493622002 2316 15 -1.8452891567110328 2316 16 -1.9221528009606672 2316 17 -1.9360048749494965 2316 18 -1.7248156059733617 2316 19 -1.7063430724814808 2316 20 -1.814947740028913 2316 21 -1.926159440304609 2316 22 -1.863037469772621 2316 23 -1.8329291515014674 2316 24 -1.8183541255522186 2316 25 -2.0505663755978034 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 43106.0 3 2.0 4 761.0 5 7145.0 6 8989.0 7 7282.0 8 5784.0 9 5370.0 10 4917.0 11 4768.0 12 4499.0 13 4402.0 14 4318.0 15 4453.0 16 4533.0 17 4709.0 18 4983.0 19 5664.0 20 7075.0 21 8994.0 22 11492.0 23 14368.0 24 18107.0 25 21348.0 26 25451.0 27 28751.0 28 33671.0 29 41182.0 30 53088.0 31 69170.0 32 88515.0 33 116386.0 34 151628.0 35 201555.0 36 292005.0 37 505359.0 38 1175857.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.98603403942532 19.4233078215695 13.423810397269968 25.166847741735214 2 15.29063075833691 20.816928327279747 39.535643697818536 24.35679721656481 3 18.334962823867514 25.045564970513635 30.229619354802022 26.389852850816826 4 13.107191488607336 17.47109312780903 37.52168705285871 31.900028330724922 5 13.655753121621938 37.554074088948234 35.41487200597049 13.375300783459338 6 31.8276461716561 36.87446210924421 18.07346387765676 13.224427841442935 7 27.996007608823266 31.62213970893943 22.432950338948526 17.94890234328878 8 25.592648354004517 36.064957423913455 20.581424403153743 17.760969818928288 9 26.55843237101409 15.327357010951314 20.19552548477483 37.91868513325977 10 15.533866923192964 28.635673232682883 33.89866564626999 21.931794197854156 11 34.23421916742455 22.449641049380755 24.447642846893338 18.86849693630136 12 23.412484316920132 24.528019153844532 31.27344099900759 20.786055530227745 13 28.836664172537123 21.74301189388722 25.812100062648696 23.608223870926956 14 21.64253314811885 21.503488492273608 27.320451940286727 29.533526419320815 15 23.354886314186064 29.83904335136086 23.390809807180485 23.41526052727259 16 22.832523939797042 27.65784511890375 26.835484784862096 22.67414615643711 17 22.293470854975787 27.9692489548237 27.357378882806128 22.379901307394388 18 22.202591776329765 27.130131013714813 28.824422088332323 21.8428551216231 19 23.853173878460815 26.73407842830317 27.223427476999255 22.189320216236755 20 23.954146370506344 26.81130165130999 27.489901116738974 21.744650861444693 21 23.509116506176063 26.346470383020026 26.959812181007575 23.18460092979633 22 23.725226085540058 27.082500609595588 27.01817949504413 22.174093809820224 23 22.98063308968464 27.029418129723947 27.304129161346992 22.685819619244423 24 23.38767190004576 26.533051371320155 27.609843426788444 22.469433301845644 25 23.233607803483967 26.760220882855755 27.419062970479047 22.587108343181235 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 789.0 1 789.0 2 794.5 3 800.0 4 800.0 5 800.0 6 1836.5 7 2873.0 8 2873.0 9 2873.0 10 4026.5 11 5180.0 12 5180.0 13 5180.0 14 8736.5 15 12293.0 16 12293.0 17 12293.0 18 22554.5 19 32816.0 20 32816.0 21 32816.0 22 51632.5 23 70449.0 24 70449.0 25 70449.0 26 100665.0 27 130881.0 28 130881.0 29 130881.0 30 167614.5 31 204348.0 32 204348.0 33 204348.0 34 235125.0 35 265902.0 36 265902.0 37 265902.0 38 292535.0 39 319168.0 40 319168.0 41 319168.0 42 342832.0 43 366496.0 44 366496.0 45 366496.0 46 376974.0 47 387452.0 48 387452.0 49 387452.0 50 381659.0 51 375866.0 52 375866.0 53 375866.0 54 342210.0 55 308554.0 56 308554.0 57 308554.0 58 266526.0 59 224498.0 60 224498.0 61 224498.0 62 184204.0 63 143910.0 64 143910.0 65 143910.0 66 111916.0 67 79922.0 68 79922.0 69 79922.0 70 58271.0 71 36620.0 72 36620.0 73 36620.0 74 25364.0 75 14108.0 76 14108.0 77 14108.0 78 9496.5 79 4885.0 80 4885.0 81 4885.0 82 3145.0 83 1405.0 84 1405.0 85 1405.0 86 896.5 87 388.0 88 388.0 89 388.0 90 228.5 91 69.0 92 69.0 93 69.0 94 41.5 95 14.0 96 14.0 97 14.0 98 7.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4355305421604335 2 0.0 3 0.0 4 0.0 5 0.055423862096600746 6 0.0 7 0.0 8 0.003277935114946816 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 3.34483174994573E-5 20 0.0 21 0.0 22 0.0 23 0.0 24 0.00531828248241371 25 0.0016055192399739503 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2989687.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.8021228211867 #Duplication Level Percentage of deduplicated Percentage of total 1 79.14081533733001 52.86774466333175 2 13.229807080875505 17.67558395034502 3 3.9249547687847635 7.8658593159588674 4 1.4793687921087564 3.9529990299311897 5 0.7299361186870105 2.4380641126074987 6 0.3836400482473409 1.5376781773286912 7 0.26325434370856277 1.2310164301141215 8 0.17591560614017268 0.9401228742031457 9 0.11649735286009394 0.700404342669281 >10 0.5188113733750515 5.697273956597202 >50 0.021571928168821336 1.0068002092679693 >100 0.012675629149057737 1.6553945598207793 >500 0.0016509723390424344 0.7921384869408221 >1k 0.0010506187609576232 1.4296391886525444 >5k 5.002946480750587E-5 0.2092807022311577 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6262 0.2094533641816016 No Hit GGTATCAACGCAGAGTACTTTTTTT 4092 0.1368705152077793 No Hit TATCAACGCAGAGTACTTTTTTTTT 4055 0.13563292746029937 No Hit GTCCTACAGTGGACATTTCTAAATT 3540 0.11840704394807886 No Hit CTTTAGGACGTGAAATATGGCGAGG 3474 0.11619945499311465 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3341 0.11175082876568684 No Hit CTGTAGGACGTGGAATATGGCAAGA 3281 0.1097439297157194 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.3448317499457303E-5 0.0 2 0.0 0.0 0.0 3.3448317499457303E-5 0.0 3 0.0 0.0 0.0 3.3448317499457303E-5 0.0 4 0.0 0.0 0.0 3.3448317499457303E-5 0.0 5 0.0 0.0 0.0 3.3448317499457303E-5 0.0 6 0.0 0.0 0.0 3.3448317499457303E-5 0.0 7 0.0 0.0 0.0 6.689663499891461E-5 0.0 8 0.0 0.0 0.0 6.689663499891461E-5 0.0 9 0.0 0.0 0.0 6.689663499891461E-5 0.0 10 0.0 0.0 0.0 6.689663499891461E-5 0.0 11 0.0 0.0 0.0 6.689663499891461E-5 0.0 12 6.689663499891461E-5 0.0 0.0 6.689663499891461E-5 3.3448317499457303E-5 13 6.689663499891461E-5 0.0 0.0 6.689663499891461E-5 3.3448317499457303E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCGTA 40 0.005291265 14.245672 7 GGTATCA 1780 0.0 12.431281 1 GTTCGCA 55 0.0030781175 12.086429 9 TACACCG 105 2.715915E-7 11.764236 5 CCGGTAC 60 0.005869148 11.085529 3 GGGCGTC 60 0.005894048 11.079226 11 TTAGGAC 1280 0.0 10.31845 3 GGACGTG 1910 0.0 10.242961 6 TGGCGAG 2010 0.0 10.159116 18 GGCGAGG 1010 0.0 10.155841 19 AGGACGT 1965 0.0 10.106328 5 TAGGACC 655 0.0 9.86455 4 CGCTCAC 135 6.481132E-7 9.848202 10 ACACCGT 145 1.6258309E-7 9.824602 6 TGTAGGA 2145 0.0 9.656925 2 GTCCTAC 1710 0.0 9.537794 1 TAGGACG 2155 0.0 9.523929 4 GACGTGA 1060 0.0 9.407519 7 ATGGCGA 2205 0.0 9.345742 17 TATGGCG 2185 0.0 9.3008995 16 >>END_MODULE