FastQCFastQC Report
Thu 26 May 2016
SRR1512128_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512128_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2298791
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50960.22168174488241865No Hit
GTCCTAAAGTGTGTATTTCTCATTT38160.1660003018978237No Hit
CTGTAGGACGTGGAATATGGCAAGA36990.16091067000001305No Hit
GTCCTACAGTGGACATTTCTAAATT36050.15682156403083186No Hit
CTTTAGGACGTGAAATATGGCGAGG35290.15351547835362153No Hit
GGTATCAACGCAGAGTACTTTTTTT34710.15099241296838206No Hit
TATCAACGCAGAGTACTTTTTTTTT32640.14198767961071712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11150.012.2504851
CGGTAGG904.723617E-612.09761
GTACTAA1653.6379788E-1211.9976211
GGCGAGG10900.011.884294519
TAGGACC7900.011.8488994
TGGCGAG23450.010.80615718
GGTCTAT1103.702764E-610.7978581
CGTGCGC909.9860365E-510.50575710
GTATTAC2001.0913936E-1110.392941
GGCGTGC1401.0387521E-710.1305528
TATGGCG25550.010.10301716
ATGGCGA26150.010.08815817
GTCCTAA13150.010.0862131
GGACGTG24050.09.9466156
AGGACGT24600.09.9164115
GGCCTAA1001.8235951E-49.8980381
GTAGGAC22600.09.8735533
GTATCAA22400.09.7212871
GTCATAT2353.6379788E-129.687441
TGTAGGA22600.09.6790542
Produced by FastQC (version 0.11.5)