##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512128_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2298791 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.620509650507593 31.0 28.0 33.0 16.0 34.0 2 26.089644513137557 31.0 25.0 33.0 10.0 34.0 3 27.748673106863563 31.0 26.0 34.0 16.0 34.0 4 32.00196886102303 35.0 32.0 37.0 19.0 37.0 5 32.395172070884215 35.0 33.0 37.0 22.0 37.0 6 32.64359221869235 35.0 33.0 37.0 25.0 37.0 7 32.68219816416543 36.0 33.0 37.0 25.0 37.0 8 32.714503406355774 36.0 33.0 37.0 25.0 37.0 9 34.224302687804155 39.0 35.0 39.0 26.0 39.0 10 34.06110777360795 38.0 34.0 39.0 25.0 39.0 11 34.075433999872104 38.0 35.0 39.0 25.0 39.0 12 33.964911120671694 38.0 34.0 39.0 25.0 39.0 13 33.95388401990438 38.0 34.0 39.0 25.0 39.0 14 34.97283659106026 39.0 35.0 41.0 25.0 41.0 15 34.90861283170153 39.0 34.0 41.0 24.0 41.0 16 34.87140109735944 39.0 34.0 41.0 24.0 41.0 17 34.806207262861214 39.0 34.0 41.0 23.0 41.0 18 34.75606003329576 39.0 34.0 41.0 23.0 41.0 19 34.77153816941166 39.0 34.0 41.0 22.0 41.0 20 34.69459555044369 39.0 34.0 41.0 21.0 41.0 21 34.618013120810026 39.0 34.0 41.0 19.0 41.0 22 34.497159158879604 39.0 34.0 41.0 18.0 41.0 23 34.37370817964747 39.0 34.0 41.0 18.0 41.0 24 34.2510519660117 39.0 34.0 41.0 17.0 41.0 25 34.117732756044376 39.0 34.0 41.0 16.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8374751466300054 1101 2 3.0806382504669223 1101 3 1.662431471041991 1101 4 1.0674515474713218 1101 5 0.6629133216444387 1101 6 0.21837579502371085 1101 7 0.0010039774900576504 1101 8 0.09460096970629905 1101 9 0.044094759200838496 1101 10 -0.06194627185125512 1101 11 0.011510831517625775 1101 12 -0.14148662833406433 1101 13 0.006868334823188604 1101 14 0.020186239180439713 1101 15 0.11183916823650719 1101 16 -1.0706581051067587E-4 1101 17 -0.1453719249099521 1101 18 -0.24832171209017417 1101 19 -0.1761387349190926 1101 20 0.014385783090560267 1101 21 0.06521577180374294 1101 22 -0.12296636543435113 1101 23 0.08775392455084585 1101 24 0.024661500058414276 1101 25 0.09403993601571159 1102 1 1.2240876972638652 1102 2 3.7623235607155365 1102 3 2.331599047869986 1102 4 1.4944512798923029 1102 5 1.1291844818434882 1102 6 1.0053892093151262 1102 7 0.7965715058509062 1102 8 0.7841584041115723 1102 9 0.8150786758508133 1102 10 0.6838044387477353 1102 11 0.8564365909624101 1102 12 0.9377054008404642 1102 13 0.9223268289423388 1102 14 0.8533620823488448 1102 15 0.7016304397753572 1102 16 0.8737605616723769 1102 17 0.8904723037135938 1102 18 0.8684113164162497 1102 19 0.8471376943988176 1102 20 0.9172255705756029 1102 21 0.8705065999018942 1102 22 0.9648454103983823 1102 23 0.7445606971608498 1102 24 0.7019279700516989 1102 25 0.7155965390748591 1103 1 0.8454745097486587 1103 2 2.5573754089507617 1103 3 1.6791829510200351 1103 4 0.9691152749527916 1103 5 0.6638932352903311 1103 6 0.4854633257788734 1103 7 0.572464315579019 1103 8 0.25554834738485255 1103 9 0.47972743704932697 1103 10 0.5227410248250024 1103 11 0.27045582589281736 1103 12 0.45167248238283975 1103 13 0.46090805071075636 1103 14 0.4743391182134076 1103 15 0.3578306295249192 1103 16 0.3164588099367762 1103 17 0.32387666763644773 1103 18 0.29274233825012885 1103 19 0.4920573361732039 1103 20 0.5628379488414339 1103 21 0.640970265714401 1103 22 0.6551682224329767 1103 23 0.5754214341405373 1103 24 0.39921817353710054 1103 25 0.40633249629956936 1104 1 1.225449707346808 1104 2 3.666200207324742 1104 3 2.1940665222560654 1104 4 1.4638873670609485 1104 5 1.1068434507815255 1104 6 0.8839568625920791 1104 7 0.6327745469958117 1104 8 0.7341502727080922 1104 9 0.8563048914956326 1104 10 0.8114674733875162 1104 11 0.8595366885392508 1104 12 0.8479042136555535 1104 13 0.7264819761207448 1104 14 0.8416935183546954 1104 15 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-2.418279994240102 2315 17 -2.377734248396173 2315 18 -2.4611121577830772 2315 19 -2.3602452740103885 2315 20 -2.513994521427378 2315 21 -2.4926627924107976 2315 22 -2.5784050073450686 2315 23 -2.3280952941064754 2315 24 -2.403797120816499 2315 25 -2.427613527085281 2316 1 -1.432075138903528 2316 2 -1.8405607786029101 2316 3 -1.1601985391168377 2316 4 -1.5909480027254013 2316 5 -1.6863581733024482 2316 6 -1.6699897808153459 2316 7 -1.6757033313350078 2316 8 -1.7300505591397979 2316 9 -2.0042983759703787 2316 10 -2.266629991964855 2316 11 -2.543749250285906 2316 12 -2.468884525927301 2316 13 -2.340229634708514 2316 14 -2.6982788549510133 2316 15 -2.8870595515594104 2316 16 -2.657518059893519 2316 17 -2.4969242039519486 2316 18 -2.4606956062839274 2316 19 -2.809944279714621 2316 20 -2.569231922379835 2316 21 -2.8683822240302455 2316 22 -2.6803572741339856 2316 23 -2.584209749598653 2316 24 -2.6856276816398363 2316 25 -3.1155172222714604 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 108569.0 3 2.0 4 882.0 5 4406.0 6 5223.0 7 4724.0 8 4609.0 9 4334.0 10 4148.0 11 4014.0 12 4060.0 13 4020.0 14 3887.0 15 4216.0 16 4295.0 17 4719.0 18 5420.0 19 6707.0 20 8965.0 21 11379.0 22 14217.0 23 16826.0 24 19420.0 25 22168.0 26 24960.0 27 28557.0 28 32515.0 29 39218.0 30 48491.0 31 61006.0 32 77378.0 33 100161.0 34 131515.0 35 177088.0 36 251758.0 37 343940.0 38 710994.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.14497968714859 19.54610053719542 13.263276217803185 25.045643557852802 2 15.729703637589026 21.90832255596641 39.808967376454945 22.553006429989615 3 18.00703065219935 25.359112681405136 30.49724833619063 26.136608330204876 4 12.259922715897183 17.67820563069892 38.91297642978418 31.14889522361972 5 13.005880047381426 37.987185437910625 36.07278782629652 12.93414668841143 6 30.601781545168745 37.74205658539641 18.788441402458943 12.867720466975902 7 27.024422837917843 32.125974044617365 23.191146998574467 17.65845611889032 8 24.45413650404626 37.20197895500631 21.33075284421493 17.013131696732497 9 26.11746783417892 15.560962262337027 21.06689994871217 37.25466995477188 10 15.07875226586497 29.070628865347047 34.86132493123559 20.989293937552393 11 33.1499470808786 22.716506198258127 25.618596905938816 18.514949814924453 12 22.953630843343305 24.94750501459245 32.34635075567983 19.752513386384408 13 28.43190181273548 22.44692971218349 26.304609683959963 22.81655879112107 14 20.672973189303562 22.703786001642605 28.16410763255704 28.459133176496792 15 22.410910778752832 31.438917239540267 23.867633029709964 22.28253895199694 16 21.62449739885009 28.363692045079347 28.439862519037177 21.57194803703338 17 21.267405896145362 28.900030015790918 28.41168614793 21.420877940133725 18 21.04306133093439 27.77085868180274 30.52709010954019 20.658989877722682 19 23.1499147811062 27.13532273121552 28.067510416367597 21.64725207131068 20 23.11396266206366 27.7976916531668 28.599244471434517 20.489101213335026 21 22.54752393542861 27.04819798598305 27.751089812940638 22.653188265647696 22 22.753078917112084 28.36684170161638 27.929957599229855 20.950121782041684 23 21.876759083723467 28.170309320327025 28.264576632320942 21.688354963628566 24 22.676326842223173 27.21167921314955 28.876123691428297 21.23587025319897 25 22.35054093675368 27.697049317249512 28.516523910405038 21.435885835591765 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 876.0 1 876.0 2 887.5 3 899.0 4 899.0 5 899.0 6 1907.0 7 2915.0 8 2915.0 9 2915.0 10 4176.5 11 5438.0 12 5438.0 13 5438.0 14 9088.5 15 12739.0 16 12739.0 17 12739.0 18 22336.0 19 31933.0 20 31933.0 21 31933.0 22 49353.5 23 66774.0 24 66774.0 25 66774.0 26 94185.0 27 121596.0 28 121596.0 29 121596.0 30 156133.0 31 190670.0 32 190670.0 33 190670.0 34 212189.5 35 233709.0 36 233709.0 37 233709.0 38 249509.0 39 265309.0 40 265309.0 41 265309.0 42 276652.5 43 287996.0 44 287996.0 45 287996.0 46 289778.0 47 291560.0 48 291560.0 49 291560.0 50 279391.5 51 267223.0 52 267223.0 53 267223.0 54 236641.0 55 206059.0 56 206059.0 57 206059.0 58 174851.5 59 143644.0 60 143644.0 61 143644.0 62 116217.0 63 88790.0 64 88790.0 65 88790.0 66 68048.0 67 47306.0 68 47306.0 69 47306.0 70 34275.5 71 21245.0 72 21245.0 73 21245.0 74 14696.0 75 8147.0 76 8147.0 77 8147.0 78 5438.5 79 2730.0 80 2730.0 81 2730.0 82 1795.5 83 861.0 84 861.0 85 861.0 86 572.5 87 284.0 88 284.0 89 284.0 90 171.0 91 58.0 92 58.0 93 58.0 94 38.5 95 19.0 96 19.0 97 19.0 98 15.0 99 11.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.473264424647565 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 8.70022546634296E-5 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 3.045078913220036E-4 15 0.0 16 0.0 17 4.35011273317148E-5 18 0.0 19 2.1750563665857403E-4 20 1.305033819951444E-4 21 8.70022546634296E-5 22 5.220135279805777E-4 23 3.9151014598543324E-4 24 2.610067639902888E-4 25 4.35011273317148E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2298791.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.1147457118214 #Duplication Level Percentage of deduplicated Percentage of total 1 84.583244273614 62.68865640819775 2 10.826620712181564 16.048244820033506 3 2.521348578242895 5.606073261820037 4 0.8765856957757248 2.598717037481515 5 0.4114452925075044 1.524708161425983 6 0.21229173389507353 0.9440368724613019 7 0.13206813322279512 0.685173727730969 8 0.08298224228163523 0.492016622824012 9 0.05675636366219666 0.37858351143162294 >10 0.26534843691793486 3.4001677014047207 >50 0.015661322476102038 0.821009497413519 >100 0.012509245494027058 1.8844265938505813 >500 0.002072244160385087 1.0785535498749574 >1k 0.0010065185921846076 1.6260141499062515 >5k 5.920697601085928E-5 0.2236180841430916 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5096 0.22168174488241865 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3816 0.1660003018978237 No Hit CTGTAGGACGTGGAATATGGCAAGA 3699 0.16091067000001305 No Hit GTCCTACAGTGGACATTTCTAAATT 3605 0.15682156403083186 No Hit CTTTAGGACGTGAAATATGGCGAGG 3529 0.15351547835362153 No Hit GGTATCAACGCAGAGTACTTTTTTT 3471 0.15099241296838206 No Hit TATCAACGCAGAGTACTTTTTTTTT 3264 0.14198767961071712 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.35011273317148E-5 0.0 5 0.0 8.70022546634296E-5 0.0 4.35011273317148E-5 0.0 6 0.0 8.70022546634296E-5 0.0 4.35011273317148E-5 0.0 7 0.0 8.70022546634296E-5 0.0 4.35011273317148E-5 0.0 8 0.0 8.70022546634296E-5 0.0 4.35011273317148E-5 0.0 9 0.0 8.70022546634296E-5 0.0 8.70022546634296E-5 0.0 10 0.0 8.70022546634296E-5 0.0 8.70022546634296E-5 0.0 11 0.0 8.70022546634296E-5 0.0 1.305033819951444E-4 0.0 12 0.0 8.70022546634296E-5 0.0 1.740045093268592E-4 0.0 13 0.0 8.70022546634296E-5 0.0 2.17505636658574E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1115 0.0 12.250485 1 CGGTAGG 90 4.723617E-6 12.0976 1 GTACTAA 165 3.6379788E-12 11.997621 1 GGCGAGG 1090 0.0 11.8842945 19 TAGGACC 790 0.0 11.848899 4 TGGCGAG 2345 0.0 10.806157 18 GGTCTAT 110 3.702764E-6 10.797858 1 CGTGCGC 90 9.9860365E-5 10.505757 10 GTATTAC 200 1.0913936E-11 10.39294 1 GGCGTGC 140 1.0387521E-7 10.130552 8 TATGGCG 2555 0.0 10.103017 16 ATGGCGA 2615 0.0 10.088158 17 GTCCTAA 1315 0.0 10.086213 1 GGACGTG 2405 0.0 9.946615 6 AGGACGT 2460 0.0 9.916411 5 GGCCTAA 100 1.8235951E-4 9.898038 1 GTAGGAC 2260 0.0 9.873553 3 GTATCAA 2240 0.0 9.721287 1 GTCATAT 235 3.6379788E-12 9.68744 1 TGTAGGA 2260 0.0 9.679054 2 >>END_MODULE