FastQCFastQC Report
Thu 26 May 2016
SRR1512128_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512128_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2298791
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67610.29411112188972377No Hit
GGTATCAACGCAGAGTACTTTTTTT45830.19936566656124896No Hit
TATCAACGCAGAGTACTTTTTTTTT45530.1980606327412975No Hit
GTCCTACAGTGGACATTTCTAAATT40950.17813711642337213No Hit
GTCCTAAAGTGTGTATTTCTCATTT40760.17731059500406954No Hit
CTTTAGGACGTGAAATATGGCGAGG40350.17552704878346923No Hit
CTGTAGGACGTGGAATATGGCAAGA39370.17126393830496117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6950.013.672294
GGTATCA14900.012.6768021
AAGGCGT1155.358743E-912.386686
ATAGGAC3200.011.2839123
AGACTCG600.00589346211.07919610
CATTTCG803.7851895E-410.68351114
AGGACCT16350.010.6355375
GGCGAGG11050.010.31356519
TACACCG750.002643216610.1357255
TGTAGGA25350.010.0082782
GTAGGAC23950.09.9981833
GGACGTG23700.09.9772876
AGGACGT23900.09.8998255
TGGCGAG25100.09.72409618
CTAGGGT2350.09.7044174
GGACCTG16300.09.6712366
TATACTG3350.09.6440665
GACGTGA13200.09.56839757
CTGTAGG24950.09.5587311
GTATCAA29500.09.5396141
Produced by FastQC (version 0.11.5)