FastQCFastQC Report
Thu 26 May 2016
SRR1512127_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512127_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3419899
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57350.16769501087605218No Hit
GGTATCAACGCAGAGTACTTTTTTT36680.10725462944958315No Hit
TATCAACGCAGAGTACTTTTTTTTT35750.10453525089483637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7200.011.4244894
GGTATCA17350.011.3583221
GTACTAG2150.010.5937571
CTCAACG1156.265145E-610.3334182
TAGGACT5250.010.2651574
GTCCTAG3700.010.1705041
GTATCAA33450.010.1545021
GGCGAGG8850.010.14916719
TAGTACC1502.6362613E-810.0850664
CGTGCGC1701.6952981E-910.01091110
GTCCTAC17150.09.931731
TCCAACG2002.7284841E-119.92748718
GTCCTAA11200.09.9028591
GTAGGAC19800.09.7890083
TGGCGAG19100.09.70225618
GGACGTG19950.09.6680156
AGGACGT20000.09.5492965
GATATAC6550.09.5248871
TCCTACA18950.09.5109252
GCCGGTT1804.5456545E-99.45474911
Produced by FastQC (version 0.11.5)