FastQCFastQC Report
Thu 26 May 2016
SRR1512127_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512127_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3419899
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69020.20181882564368128No Hit
TATCAACGCAGAGTACTTTTTTTTT46030.13459461814515575No Hit
GGTATCAACGCAGAGTACTTTTTTT44850.1311442238498856No Hit
GTCCTACAGTGGACATTTCTAAATT35650.10424284459862705No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGA400.00529377714.24473610
GTTTACG655.25031E-513.2078851
AACCGTC658.0496987E-411.6885017
TAGGACC7000.011.4024584
GGTATCA20250.011.2584081
TAGAGCG803.7656623E-410.6898045
GGCGAGG9050.010.28424519
GTCCTAA11300.09.9611081
GTCTAAG2900.09.867961
TGTAGGA22000.09.7180042
AGGACGT19500.09.6969615
GTATCAA40350.09.5981291
GGACGTG19200.09.5958346
TTAGGAC13100.09.5745833
GTAGGAC20600.09.50204853
CGTACTG800.00450976439.5020485
GACGTGA11100.09.3257917
TAAGCCC1856.681148E-99.2452364
TAGAACA9100.09.1887944
TGGCGAG19550.09.18088518
Produced by FastQC (version 0.11.5)