FastQCFastQC Report
Thu 26 May 2016
SRR1512126_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512126_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2369843
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55120.23258924747335585No Hit
GGTATCAACGCAGAGTACTTTTTTT37370.15768977101014708No Hit
TATCAACGCAGAGTACTTTTTTTTT36000.15190879733383184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14900.013.1517471
GTTATAT1353.6779966E-710.2635771
ACCGTGC1804.292815E-109.9806758
GTGTAGG3500.09.8970211
CTAATAC1451.7328239E-79.7814243
GTATCAA28950.09.6406211
AGACCGC900.00116297189.4553766
GGACCGA900.00116297189.4553766
ACCCCGC800.00469575789.4553768
GTATAAG2457.2759576E-129.291081
TAGGACG4300.09.2354834
GTTTAGC1302.6994068E-59.1357121
GTATTAG1853.38714E-89.094561
TAGGACC2600.09.0917074
ACCGCCC1054.7101302E-49.005128
AGGACGT4100.08.9941385
ACCGATC950.00190401768.9577268
GTCAACG1000.00200417998.9073192
TTAGGAC3550.08.7895043
GTATTAA2501.0186341E-108.7093781
Produced by FastQC (version 0.11.5)