FastQCFastQC Report
Thu 26 May 2016
SRR1512126_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512126_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2369843
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67530.28495558566537954No Hit
GGTATCAACGCAGAGTACTTTTTTT47400.20001324982287855No Hit
TATCAACGCAGAGTACTTTTTTTTT47170.19904272139546797No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATT500.001503811313.29546513
GGTATCA16600.011.4329171
CTTAGAC1451.4402758E-810.4847153
CGTATGC1002.4088777E-510.44643810
GTCCTAC3600.010.3317561
GTATTAC1701.4588295E-910.0980051
GTTCTAA2450.09.7316381
GTCTTAG2600.09.5370051
TTATACT3100.09.5017734
TATGCGA800.0045296489.49676112
TACCGTA900.00111744689.4967617
GTATCAA32450.09.3753611
GTATAGG2159.822543E-119.3152141
GTCCTAT1951.5697879E-99.2924671
AATCCCG1651.0702752E-79.20975819
GGTATAC1252.6314789E-59.1555251
TATACTG4650.08.9909255
GTCTATA2356.002665E-118.9282611
TTTATAC2800.08.8230743
GTACCGT1304.387377E-58.7662426
Produced by FastQC (version 0.11.5)